Procyanidine extraction method

An extraction method and proanthocyanidin technology, applied in the direction of organic chemistry and the like, can solve the problems that cannot be widely applied, the price of beauty products is high, and the extraction rate is not high, and the effects of not easy to be oxidized, less impurities, and high extraction efficiency are achieved.

Inactive Publication Date: 2017-08-11
北海市韩能生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the high antioxidant properties of proanthocyanidins, they are rapidly applied in beauty products. Antioxidants are added to various types of skin care products to improve the protection of the products on the skin; however, the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for extracting proanthocyanidins, comprising the following steps:

[0015] (1) Take the washed and dried grape seeds and grape skins, mix them evenly at a ratio of 14:4, and crush them into powder;

[0016] ⑵, step ⑴ powder, add to the mixed extraction solution, the mixed extraction solution is composed of ethanol, ethyl acetate and water with a volume ratio of 14:8:4; stir evenly;

[0017] (3) Step (2) Mix the material, extract it with 3.2MHz ultrasonic waves for 35 minutes at 9MPa and 19°C in a carbon dioxide environment, quickly cool to room temperature, filter, and vacuum-dry the filtrate.

[0018] The product is detected by liquid chromatography, and the relative content is 80%.

Embodiment 2

[0020] A method for extracting proanthocyanidins, comprising the following steps:

[0021] (1) Take the grape seeds and grape skins after washing and drying, mix them evenly in a ratio of 12:6, and crush them into powder;

[0022] (2) Step (1) powder is added to the mixed extract, which is composed of ethanol, ethyl acetate and water with a volume ratio of 12:9:3; stir evenly;

[0023] (3) Step (2) The mixed material is extracted at 10MPa and 15°C in a helium environment with a 3.5MHz ultrasonic wave for 30 minutes, rapidly cooled to room temperature, filtered, and the filtrate is vacuum-dried.

[0024] The product is detected by liquid chromatography, and the relative content is 82%.

Embodiment 3

[0026] A method for extracting proanthocyanidins, comprising the following steps:

[0027] (1) Take the washed and dried grape seeds and grape skins, mix them evenly at a ratio of 15:2, and crush them into powder;

[0028] (2) Step (1) powder is added to the mixed extract, which is composed of ethanol, ethyl acetate and water with a volume ratio of 16:6:5; stir evenly;

[0029] (3) Step (2) Mix the material, extract it with 3MHz ultrasonic waves for 50 minutes at 8MPa, 22°C, and isolate oxygen for 50 minutes, quickly cool to room temperature, filter, and vacuum-dry the filtrate.

[0030] The product is detected by liquid chromatography, and the relative content is 83%.

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PUM

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Abstract

The invention discloses a procyanidine extraction method. The extraction method comprises the following steps: 1) taking washed and dried grape seeds and grape skin, and uniformly mixing the grape seeds and grape skin according to the proportion of 12-15:2-6, fragmenting the mixture to powder; 2) adding powder in the step 1) in a mixing extract, wherein the mixing extract is formed by uniformly mixing ethanol, ethyl acetate and water according to volume ratio of 12-16: 6-9: 3-5; and uniformly stirring the materials; and 3) performing ultrasonic extraction on the mixture in the step 2) for 30-50 min at condition of 8-10 MPa, 3-3.5 MHz ultrasonic wave and at 15-22 DEG C, quickly cooling the material to room temperature, filtering the material, and performing vacuum drying on a filtrate. The method employs waste material grape seed and grape skin which cannot be directly eaten, the waste material grape seed and grape skin are taken as the preparation raw materials of procyanidine, the waste can be used, cost is saved, under effect of a mixed solvent, the procyanidine can be dissolved, and is extracted under high pressure and low temperature through ultrasonic wave, the procyanidine is difficulty oxidized, the extraction efficiency is high, impurity is little, and the speed is fast.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting proanthocyanidins. Background technique [0002] Proanthocyanidins, the English name is Oligomeric Proantho Cyanidins (OPC), is a bioflavonoid with a special molecular structure, is currently internationally recognized as the most effective natural antioxidant for removing free radicals in the human body, and has very strong in vivo activity. Experiments have shown that , OPC's ability to resist free radical oxidation is 50 times that of vitamin E and 20 times that of vitamin C, and it can be absorbed quickly and completely. Its preparation route is usually through the extraction of grape seeds, using water extraction or organic solvent extraction. [0003] Due to the high antioxidant properties of proanthocyanidins, they are rapidly applied in beauty products. Antioxidants are added to various types of skin care products to improve the pr...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 万明坤
Owner 北海市韩能生物科技有限公司
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