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Biochemical method for detecting ionizing radiation dose

A technology of ionizing radiation and biology, applied in the field of biological detection of radiation dose, can solve the problems of high detection cost, complicated operation, and many subjective factors, and achieve the effect of improving accuracy

Inactive Publication Date: 2012-06-20
FUDAN UNIV
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AI Technical Summary

Problems solved by technology

However, the common disadvantages of these methods are that there are complex operations, many subjective factors, high detection costs, and only high-dose radiation can be detected and measured.
For the biological detection and measurement of low-dose radiation, there is currently no accurate and sensitive method

Method used

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Embodiment Construction

[0016] 1) Irradiation conditions and experimental grouping: HMy2.CIR human B lymphoblastoid cells in the logarithmic growth phase (1×10 6 Cells / dish) were seeded in 60mm culture dishes, cultured for 24h, and the cells were irradiated with low doses of 1.6, 3.2, 4.8, 6.4, 8cGy or high doses of 0.4, 1, 2, 4, 8, 12, 16, 20Gy at room temperature Dose irradiation, and set up a blank group without irradiation at the same time, each group set up three repetitions (n=3).

[0017] 2) Immediately after the cells were irradiated, the cells were incubated in fresh culture medium containing 3 μg / ml cytochalasin B (Sigma Company) and continued to culture for 24 hours.

[0018] 3) Transfer the cells into a 15ml centrifuge tube, centrifuge at 300g for 10min to remove the culture medium, add 7ml 0.075M KCl to each tube, mix well, and hypotonic at room temperature for 10min.

[0019] 4) Add 1ml of fixative solution (by mass ratio, methanol: glacial acetic acid 9:1) to pre-fix the cells.

[00...

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Abstract

The invention belongs to the technical field of biological detection, in particular to a biochemical method for detecting the ionizing radiation dose. According to the principle that a cell cycle can be interfered by ionizing radiation and cytochalasin B can retard cell division but cannot retard cell nucleus division, after different doses of gamma rays are used for irradiating lymphocyte, a certain quantity of cytochalasin B is added for processing so as to detect the four-core rate and the double-core rate of the lymphocyte irradiated with different doses of gamma rays. An experiment result shows that the four-core rate of the irradiated lymphocyte and the irradiated dose are in a linear dose-effect relationship within a low-dose range of 0-6.4cGY, a relevant coefficient is 0.957, and the radiation excitability effect is presented; and within a high-dose range of 0.4-20Gy, the double-core rate of the irradiated lymphocyte and the irradiated dose are in an exponential equation dose-effect relationship, the relevant coefficient is 0.978, and the radiation damage effect can be favorably reflected. The biochemical method for detecting the ionizing radiation dose is visual in detection indexes, is simple to operate, is quick and economic and is accurate in detection result.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a biological detection method of radiation dose. Background technique [0002] To assess the health hazards of ionizing radiation, and to diagnose and treat acute and chronic radiation injuries, it is necessary to understand and determine the exposure dose. There is a certain dose-effect relationship between the changes in body cells, DNA, proteins, etc. after ionizing radiation and the radiation dose. These detectable biological indicators can be used to estimate the size of the radiation dose. Compared with physical detection, the biggest advantage of biological dosimetry is that it directly reflects the body's response to ionizing radiation. [0003] At present, there are many methods for biological detection of radiation dose, and the commonly used ones are: the micronucleus method and the chromosome karyotype analysis method proposed by detecting th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01T1/02C12Q1/02
Inventor 邵春林袁德晓叶爽潘燕张江虹白杨
Owner FUDAN UNIV
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