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Application of high mobility group box 1 (HMGB1) as biological dosemeter of ionizing radiation

A high mobility, biological dose technology, applied in dosimeters and other directions, can solve the problems of time-consuming, expensive and laborious

Inactive Publication Date: 2011-02-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, a lot of work has been carried out in the field of radiation biological dosimetry. Although a variety of radiation biological dosimeters have been established, there are problems such as time-consuming, laborious, and expensive in practical applications, which are still not ideal.
Therefore, recently, domestic and foreign related laboratories have carried out some new biological dosimeter research, such as GADD45 (growth arrest and DNA damage gene 45) gene expression measurement, mitochondrial DNA gene mutation analysis, etc., so far these studies are still in the stage of exploration and trial , so it is necessary to find a new, fast and reliable ionizing radiation biological dose index

Method used

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  • Application of high mobility group box 1 (HMGB1) as biological dosemeter of ionizing radiation
  • Application of high mobility group box 1 (HMGB1) as biological dosemeter of ionizing radiation
  • Application of high mobility group box 1 (HMGB1) as biological dosemeter of ionizing radiation

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Dose-effect relationship of HMGB1 protein release in culture medium of mouse fibroblasts cultured in vitro with different doses of ionizing radiation

[0020] (1) Materials: mouse fibroblast cell line L929 was purchased from the American Cell Collection Center (ATCC), and was preserved and cultured by our laboratory; RPMI 1640 medium dry powder, calf serum, and trypsin were purchased from Gibco; amicon ultrafiltration Centrifuge tubes were purchased from Millipore, USA. Standard protein molecular weight, SDS-PAGE loading buffer, RIPA protein lysate, TE electrophoresis buffer, 10× transfer solution, 30% Acry-Bis, Tris-Hcl, ammonium persulfate (AP), SDS, tetramethyl Ethylenediamine (TEMED) was purchased from Jiangsu Beyantian Biotechnology Research Institute; dimethyl sulfoxide (DMSO), agarose and Tween-20 were purchased from Shanghai Hi-Tech Bioengineering Co., Ltd.; anti-HMGB1 and other antibodies were purchased from Santa Cruzs Biotechnology, USA technology company. ...

Embodiment 2

[0026] Dose-effect relationship of HMGB1 protein release in human peripheral anticoagulated whole blood with different doses of ionizing radiation

[0027] (1) Human peripheral blood collection, human anticoagulant peripheral venous blood used in the experiment came from 3 healthy blood donors, 1 male and 2 female, aged about 30 years old, requirements: no smoking, no drinking, good health, no history of chronic diseases, within half a year Free from irradiated and inflammatory diseases. 20ml of blood was collected from each person, divided into 6 vials, and subjected to 0, 3, and 6Gy of x-rays at room temperature, and then left to stand at 37°C for 4h and 24h to collect plasma samples.

[0028] (2) ELISA method to determine the amount of HMGB1 protein in the human peripheral anticoagulated whole blood sample obtained in step (1): For the operation steps, refer to the kit instruction manual. Afterwards, it was reacted at 37°C for 2 hours, the sample solution was discarded, an...

Embodiment 3

[0033] (1) GM cells were cultured in RPMI 1640 medium containing 10% calf serum, glutamine and 1 million U / L penicillin and streptomycin.

[0034] (2) using different dosage levels 60 Co gamma ray irradiation step (1) obtained GM cell culture fluid, according to the method of embodiment two, adopts ELISA method to detect the change of HMGB1 protein content in the cell culture fluid after irradiation: 24h directly collects the supernatant of cell culture fluid after irradiation, according to the implementation The method of Example 2, using ELISA method to detect the content of HMGB1 wherein, the results are shown in Table 1, Figure 4 shown.

[0035] As shown in Table 1, the content of HMGB1 in the culture medium gradually increased with the increase of irradiation dose: compared with the unirradiated control group, the content of HMGB1 in each irradiation dose group increased significantly (0.5Gy, 1Gy, 2Gy and 4Gy irradiation groups P <0.05, P<0.01 in the 8Gy irradiation gr...

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Abstract

The invention belongs to a biological dosemeter of ionizing radiation, in particular to application of high mobility group box 1 (HMGB1) as a biological dosemeter of ionizing radiation. After isolated cells especial blood is radiated, the increase of HMGB1 content is proportional to received ionizing radiation dose to form certain dose-effect relation, and the ELISA (Enzyme-Linked Immuno Sorbent Assay) method and other simple, convenient and feasible methods can be adopted for rapid detection after 24 h of irradiation. Thus, the HMGB1 can be used as the biological dosemeter of ionizing radiation; moreover, the HMGB1 can be used for measuring the biological radiation dose of human bodies and animals exposed to ionizing radiation.

Description

technical field [0001] The invention belongs to ionizing radiation biological dosimeters, in particular to the application of high mobility group box 1 (HMGB1) as ionizing radiation biological dosimeters. Background technique [0002] The peaceful application of nuclear energy and the extensive application of radionuclides in various fields such as medicine, industry and agriculture have brought enormous benefits to mankind. While nuclear technology benefits mankind, it also poses a potential threat to public safety and public health. Therefore, correctly estimating the exposure dose of radiation accident victims and tumor radiotherapy patients is conducive to improving radiation protection measures, guiding clinical treatment, and predicting disease progression. Over the years, a lot of work has been carried out in the field of radiation biological dosimetry. Although a variety of radiation biological dosimeters have been established, they are still not ideal due to the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01T1/02
Inventor 焦旸樊赛军
Owner SUZHOU UNIV
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