Method of establishing Hormesis dose-effect fitting model

Abstract

The invention belongs to the field of environment pollution detection technology and relates to a method of establishing a Hormesis dose-effect fitting model, namely a Bilogistic model. The method comprises the following steps: (1) analyzing the stability of a compound; (2) recovering and culturing strains; (3) conducting toxicity test including acute toxicity test and chronic toxicity test; (4) calculating the inhibition ratio of the compound analyzed in the step (1) to the strains recovered and cultured in the step (2) by applying the experiment result obtained in the step (3), and conducting toxicity representation by taking inhibition ratio as an index; and (5) fitting the toxicity data to obtain the Bilogistic model. The data fitting is more convenient through the model; parameters in the model are initialized simply; the model is established based on a receptor mechanism hypothesis, thereby having great biological significance; good fitting can be conducted under the condition of high stimulatory effect; the Hormesis effect can be effectively guided to the application in the drug design.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution detection, and relates to a method for establishing a Hormesis dose-effect fitting model, that is, a Bilogistic model. Background technique [0002] In the environmental field, traditional toxicology research focuses on the high-dose area of ​​poisons, and its main purpose is to evaluate the toxicity of poisons and determine the dose threshold for human safety. Therefore, traditional ecological risk assessment models mainly include threshold models and linear models (such as figure 2 a and 2b). Hormesis refers to the special phenomenon that the dose-response relationship of a poison or a compound to an organism shows an inhibitory effect at a high dose and a stimulating effect at a low dose [1], this two-way dose-effect model ( figure 2 c) Undoubtedly, it poses a challenge to the classic dose-response model that has been used in toxicological safety evaluation [2], which will fun...

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Problems solved by technology

[0006] (2) Quantitative research: Due to the uncertainty of the existing Hormesis mechanism, the quantitative research of Hormesis is progressing slowly, and no unified and convincing evaluation method has been formed so far.

In the past few decades, scholars have successively proposed many dose-response models for fitting the Hormesis phenomenon, but most of them are empirical models and thus have many deficiencies [7] The research on the Hormesis effect of mixtures is still in its infancy

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