The invention discloses a detection method of a Qingjin Qi-tonifying composition and a fingerprint construction method of the Qingjin Qi-tonifying composition. The detection method of the Qingjin Qi-tonifying composition comprises the following steps: S1, preparing isoferulic acid, hesperidin and ammonium glycyrrhizinate: a, preparing a mixed reference solution; b, preparing a test solution; c, preparing a negative composition solution; d, determining chromatographic conditions; and e, respectively injecting the solutions into a liquid chromatograph, and determining to obtain the target product. S2, preparing verbenin, verbenin, baicalin and wogonoside of the radix morindae officinalis; a, preparing a mixed reference substance solution; b, preparing a test solution; c, preparing a negative composition solution; d, determining chromatographic conditions; and e, respectively injecting the solutions into a liquid chromatograph, and determining. According to the detection method of the Qingjin Qi-tonifying composition, quality control is conducted on the Qingjin Qi-tonifying composition, and it is guaranteed that medication of a patient is safe, reliable and effective.