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Method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis aqueous extract

A technology for separating and purifying isoferulic acid, applied in the field of chemical industry, can solve the problems of few research reports on chemical components, and achieve the effects of saving reagents, high purity and short process cycle.

Inactive Publication Date: 2015-04-29
LIAOCHENG UNIV
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Problems solved by technology

[0003] Since propolis is easily soluble in ethanol, there are many reports on the chemical components of the fat-soluble parts of Chinese propolis, but there are relatively few reports on the chemical components of the water-soluble parts

Method used

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  • Method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis aqueous extract
  • Method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis aqueous extract
  • Method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis aqueous extract

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Embodiment

[0032] Example: The method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis water extract, its steps are:

[0033] (1) Preparation of Chinese propolis water extract: Chinese propolis was frozen, pulverized, heated and extracted 4 times with 8 times the amount of distilled water in a water bath at 80 °C, 2 h each time, filtered, and the water extract was cooled naturally, and the solidified supernatant was removed. The beeswax is then concentrated under reduced pressure to a relative density of about 1.2.

[0034] (2) Alcohol precipitation: add about 2 times the amount of 95% ethanol to the above concentrated solution until the ethanol content is about 70%, let stand overnight, take the supernatant and concentrate to obtain the extract.

[0035] (3) Coarse separation of macroporous adsorption resin: Dissolve the above extract with an appropriate amount of water and then apply it to HPD826 macroporous adsorption resin column, el...

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Abstract

The invention relates to a method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from a Chinese propolis aqueous extract. The method adopts Chinese propolis as a raw material, and comprises the following steps: (1) preparing a Chinese propolis aqueous extract; (2) precipitating with alcohol; (3) coarsely separating with macroporous adsorption resin; and (4) separating and purifying by semi-preparative high performance liquid chromatography. According to the method, the semi-preparative high performance liquid chromatography is used for separating and purifying, a mobile phase is methanol-water, and three kinds of high-purity ingredients, which are identified to be caffeic acid, ferulic acid and isoferulic acid respectively, are obtained. The method is green and environment-friendly in process, does not have serious damage to the environment, and has low comprehensive cost.

Description

technical field [0001] The invention belongs to the field of chemical industry, in particular to a method for separating and purifying caffeic acid, ferulic acid and isoferulic acid from Chinese propolis water extract. Background technique [0002] Chinese propolis has a wide range of pharmacological activities, such as anti-tumor, anti-bacterial, anti-viral, anti-inflammatory, anti-oxidation, lowering blood fat, lowering blood sugar, etc., so it is widely used in food and health care products industry. The chemical composition of Chinese propolis is extremely complex. So far, at least 20 categories and 300 chemical components have been isolated and identified, including phenolic acids, flavonoids, amino acids, steroids and other substances, among which phenolic acids and flavonoids are the main active components. The fat-soluble part of Chinese propolis mainly contains flavonoids, while the water-soluble part mainly contains organic acids (such as caffeic acid, ferulic acid...

Claims

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Application Information

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IPC IPC(8): C07C59/52C07C59/64C07C51/42C07C51/47
CPCC07C51/42C07C51/47C07C51/48C07C59/52C07C59/64
Inventor 李爱峰玄红专柳仁民孙爱玲
Owner LIAOCHENG UNIV
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