Detection method of Qingjin Qi-tonifying composition and fingerprint construction method of Qingjin Qi-tonifying composition

A technology of fingerprint spectrum and composition, which is used in measuring devices, instruments, scientific instruments and other directions to achieve the effect of drug safety

Active Publication Date: 2022-06-10
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no report on the quality control method of Qingjin Yiqi composition with multi-component content determination and fingerprint spectrum

Method used

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  • Detection method of Qingjin Qi-tonifying composition and fingerprint construction method of Qingjin Qi-tonifying composition
  • Detection method of Qingjin Qi-tonifying composition and fingerprint construction method of Qingjin Qi-tonifying composition
  • Detection method of Qingjin Qi-tonifying composition and fingerprint construction method of Qingjin Qi-tonifying composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] a Mixed reference substance solution preparation: Accurately weigh the reference substances isoferulic acid, hesperidin and ammonium glycyrrhizinate, add methanol to prepare 10.28 μg of isoferulic acid, 75.04 μg of hesperidin and 41.92 μg of ammonium glycyrrhizinate per 1 mL The solution;

[0056] b Preparation of the test solution of the Qingjin Yiqi Composition: Take 0.50g of the Qingjin Yiqi Composition, put it into a 50mL Erlenmeyer flask, add 25mL of a 70% aqueous methanol solution, ultrasonically dissolve it for 20min, and let it cool to make up Lose weight, shake well, take the supernatant and pass through a 0.22 μm filter membrane to obtain;

[0057] c. Preparation of the negative composition solution of clearing gold and replenishing Qi: take the negative composition of clearing gold and replenishing Qi and lacking Cimicifuga, the negative composition of clearing gold and replenishing Qi and lacking tangerine peel, and the negative composition of clearing gold ...

Embodiment 2

[0084] a'Preparation of the mixed reference substance solution: Accurately weigh the reference substances Verbenain, Verbenain, Baicalin, and Wogonoside, and add methanol to prepare each 1mL containing Verbenain 10.00 μg and Verbenain 10.14 μg, baicalin 65.7μg, wogonoside 20.04μg solution;

[0085] b'Preparation of test solution of Qingjin Yiqi Composition: Take 0.50g of Qingjin Yiqi Composition, put it into a 50mL Erlenmeyer flask, add 25mL of 70% methanol aqueous solution precisely, ultrasonically dissolve it for 20min, let it cool Make up for the weight loss, shake well, take the supernatant and pass it through a 0.22 μm filter membrane, accurately absorb 5 mL of the filtrate, put it in a 25 mL volumetric flask, add a 70% aqueous methanol solution, and constant volume, to get ready;

[0086] c'Preparation of the negative composition solution of Qingjin Yiqi: take the negative composition of Qingjin Yiqi Deficient Verbena and the negative composition of Qingjin Yiqi Deficien...

Embodiment 3

[0115] a"Preparation of the mixed reference solution: Accurately weigh the reference substances verbenin, verbenin, baicalin, wogonin, isoferulic acid, hesperidin and ammonium glycyrrhizinate respectively, add methanol, and prepare Each 1mL solution contains 13 μg of verbenin, 13.5 μg of verbenin, 150.5 μg of baicalin, 30 μg of wogonin, 7.4 μg of isoferulic acid, 73 μg of hesperidin and 15.6 μg of ammonium glycyrrhizinate;

[0116] b "Preparation of the test solution: take 9 batches of Qingjin Yiqi composition, each 0.50g, respectively placed in a stoppered conical flask, add 25mL volume concentration of 70% methanol aqueous solution, ultrasonic 20min at 400W , get the supernatant to obtain 9 batches of test solution; (7,8,10 and above batches can also be used)

[0117] c″ determination: Accurately measure 10 μL each of the mixed reference substance solution and 9 batches of the test solution, respectively, and use high-performance liquid chromatography for detection. The chro...

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Abstract

The invention discloses a detection method of a Qingjin Qi-tonifying composition and a fingerprint construction method of the Qingjin Qi-tonifying composition. The detection method of the Qingjin Qi-tonifying composition comprises the following steps: S1, preparing isoferulic acid, hesperidin and ammonium glycyrrhizinate: a, preparing a mixed reference solution; b, preparing a test solution; c, preparing a negative composition solution; d, determining chromatographic conditions; and e, respectively injecting the solutions into a liquid chromatograph, and determining to obtain the target product. S2, preparing verbenin, verbenin, baicalin and wogonoside of the radix morindae officinalis; a, preparing a mixed reference substance solution; b, preparing a test solution; c, preparing a negative composition solution; d, determining chromatographic conditions; and e, respectively injecting the solutions into a liquid chromatograph, and determining. According to the detection method of the Qingjin Qi-tonifying composition, quality control is conducted on the Qingjin Qi-tonifying composition, and it is guaranteed that medication of a patient is safe, reliable and effective.

Description

technical field [0001] The technical field of detection of traditional Chinese medicine compositions of the present invention, in particular, relates to a detection method of Qingjin Yiqi composition and a method for constructing fingerprints thereof. Background technique [0002] Qingjin Yiqi composition is composed of 16 traditional Chinese medicines such as ginseng, Ophiopogon japonicus and Schisandra chinense. Laxity, dry cough with less phlegm, unfavorable throat, shortness of breath after movement, fatigue, fullness in the chest and abdomen, anorexia and soft stools, heavy limbs, pale tongue and less fluid. [0003] The composition of Qingjin Yiqi has many medicinal flavors and complex components. The quantitative analysis of a single active component is difficult to characterize the overall quality of the compound and cannot fully control its quality. At present, TCM fingerprints are an important and authoritative method for the quality control of TCM compound prescr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686G01N2030/027Y02A50/30
Inventor 刘志东张伯礼张俊华祁东利王跃飞邓秀平常艳旭惠玉玉
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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