The invention provides an ecological breeding feed for mixed fish and belongs to the technical field of fish feed processing. The feed is mainly prepared by processing sunflower seed cake, peanut cake, fish meal, corn gluten meal, residue of monosodium glutamate, linseed cake, bagasse powder, molasses yeast powder, bone meal, stone powder, rare earth, alpha-starch, sodium alginate, fresh milk flavor, salt, vitamin additives and traditional Chinese medicine additives according to a certain mass ratio. The traditional Chinese medicine additives are mainly prepared from Chinese mahonia (Mohonia fortunei), capillary artemisia, Paris polyphylla, cortex meliae, dichroae radix, stone-like omphalia, all-grass of Blackspiked Lovegrass, rosa laevigata michx, clovershrub, root of vitex negundo linn, lithargite, ailanthus altissima, asiatic toddalia root and dioscorea nipponica makino. The ecological breeding feed for mixed fish has balanced and comprehensive nutrients, and is suitable for a variety of environments and fish. By the addition of the traditional Chinese medicine additives, disease resistance of fish can be enhanced, and survival rate can be boosted. The feed has good palatability and high convention rate. By the use of the feed, food consumption of fish can be increased, and growth speed of fish can be accelerated. Meanwhile, utilization rate of the feed is boosted, feeding amount of the feed is reduced, and feeding cost is reduced.