Method for separating greater celandine, eomecon chionantha hance or toddalia asiatica total alkaloids

A technology of flying dragon palm blood and total alkaloids, applied in the directions of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of hidden dangers of environmental sanitation, cumbersome steps, low transfer rate, etc., and reduce the process of regenerating resin columns. , The effect of high alkaloid transfer rate and simple process flow

Inactive Publication Date: 2009-07-29
BEIJING HERUN INNOVATION PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has some progress compared with other conventional extraction and separation methods, there are still serious defects: in the test, it can be found that although the method can improve the purity of alkaloids, the transfer rate is still not high; Alkali needs to have better solubility in ethanol solution, but the use of ethanol solution will bring residues of resin monomer components such as styrene and divinylbenzene into the eluent, which will affect the safety of the preparation; after passing through the resin The obtained a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 1000g of celandine decoction pieces, add water and decoct twice, 10 times the amount each time, decoct for 2 hours, combine the decoction liquid, centrifuge, adjust the pH=3, centrifuge, and the supernatant passes through the cation at 4 times the column volume / hour Exchange resin (001×7, hydrogen type, diameter-to-height ratio 1:8), wash with water until the effluent is colorless, then elute with 15% hydrochloric acid solution, the elution rate is 2 times column volume / hour, collect the eluate, When the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), it is neutralized to neutral with sodium hydroxide, desalted, and the filtrate is concentrated and dried to obtain the total alkaloids of celandine.

Embodiment 2

[0036] Take 1000g of Feilongzhang blood decoction pieces, add pH=5 acid water for percolation, combine the percolation liquid and add water to dilute to 10000ml, centrifuge, adjust pH=1, centrifuge, supernatant passes through cation exchange resin ( Doulex 50, sodium type, diameter-to-height ratio 1:5), washed with water until the effluent was colorless, then eluted with 5% hydrochloric acid solution, the elution rate was 4 times the column volume / hour, after elution was 2 times the column volume, soaked for 5 Hours, then elute with 5% hydrochloric acid solution, collect the acid eluate until the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), neutralize with calcium hydroxide to neutral, and remove salt. The filtrate was concentrated and dried to obtain the total alkaloids in the blood of Dragon's Palm.

Embodiment 3

[0038] Take 5 kg of Xueshuicao decoction pieces, add 70% ethanol to ultrasonically extract twice, add 5 times the amount each time, ultrasonically for 0.5 hours, combine the extracts, centrifuge, recover ethanol, add water to 5000ml, adjust pH = 2, centrifuge, and the supernatant to 2 times the column volume / hour, pass through the upper cation exchange resin (001×10, sodium type, diameter-to-height ratio 1:7), wash with water until the effluent is colorless, and then wash with 1% hydrochloric acid solution (containing 1% sodium chloride) The elution rate is 5 times the column volume / hour, until the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), this part of the eluate is collected, neutralized to neutral with sodium hydroxide, Desalination, the filtrate is concentrated and dried to obtain the total alkaloids of the blood water grass.

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Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely greater celandine, snowpoppy or asiatic toddalia root, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the greater celandine, the snowpoppy or the asiatic toddalia root; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; checking eluent until no alkaloid exists; collecting the eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and performing concentrated drying to obtain the greater celandine, snowpoppy or asiatic toddalia root total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the greater celandine, snowpoppy or asiatic toddalia root total alkaloid from the extracting solution of the greater celandine, the snowpoppy or the asiatic toddalia root.

Description

technical field [0001] The present invention relates to a method for extracting and separating the effective components of traditional Chinese medicine, in particular to a method for extracting and separating the total alkaloids of celandine, bloodweed or Feilongzhang blood from the Chinese medicine celandine, bloodweed or Feilongzhang blood extract , belongs to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Studies have shown that total alkaloids in celandine, blood water grass or dragon palm blood are the main anticancer active parts of the three. Celandine is the whole herb of Chelidonium majus L., a plant of the papium family. It is a commonly used Chinese herbal medicine. It is cool in nature, bitter in taste, and slightly toxic. It has the e...

Claims

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Application Information

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IPC IPC(8): A61K36/66A61K36/75A61P35/00C07G5/00A61K125/00A61K127/00
Inventor 武建卓朴美善柴金苗
Owner BEIJING HERUN INNOVATION PHARMA TECH DEV
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