Method for separating greater celandine, eomecon chionantha hance or toddalia asiatica total alkaloids

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely greater celandine, snowpoppy or asiatic toddalia root, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the greater celandine, the snowpoppy or the asiatic toddalia root; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; checking eluent until no alkaloid exists; collecting the eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and performing concentrated drying to obtain the greater celandine, snowpoppy or asiatic toddalia root total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the greater celandine, snowpoppy or asiatic toddalia root total alkaloid from the extracting solution of the greater celandine, the snowpoppy or the asiatic toddalia root.

Description

technical field [0001] The present invention relates to a method for extracting and separating the effective components of traditional Chinese medicine, in particular to a method for extracting and separating the total alkaloids of celandine, bloodweed or Feilongzhang blood from the Chinese medicine celandine, bloodweed or Feilongzhang blood extract , belongs to the field of traditional Chinese medicine. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Studies have shown that total alkaloids in celandine, blood water grass or dragon palm blood are the main anticancer active parts of the three. Celandine is the whole herb of Chelidonium majus L., a plant of the papium family. It is a commonly used Chinese herbal medicine. It is cool in nature, bitter in taste, and slightly toxic. It has the e...

AI Technical Summary

Problems solved by technology

Although this method has some progress compared with other conventional extraction and separation methods, there are still serious defects: in the test, it can be found that although the method can improve the purity of alkaloids, the transfer rate is still not high; Alkali needs to have better solubility in ethanol solution, but the use of ethanol solution will bring residues of resin monomer components such as styrene and divinylbenzene into the eluent, which will affect the safety of the preparation; after passing through the resin The obtained aqueous solution has undergone two acid-base conversions and one chloroform extraction. Not only the steps are cumbersome, but also a large amount of chloroform is used, which greatly increases the production cost and brings safety to environmental sanitation, test operations and final preparations. Hidden dangers; at the same time, because ethanol is used in the process, it is also required to increase the ethanol treatment step during resin pretreatment, and to recycle ethanol later, which increases the process steps and increases the production cost