14 results about "Ethanol treatment" patented technology

The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.

The present application relates to a kind of methods for synthesizing alkyl galactoside using immobilized cells with monosaccharide as glycosyl donor.The method comprises the following steps: (1) culturing beta-galactosidase-producing yeast; (2) treating the yeast cells with ethanol; (3) adding genipin to the ethanol-treated yeast cells to construct a cross-linking system; reacting the cross-linking system for 0.5-4 h, centrifuging to obtain immobilized cells; (4) mixing galactose and alkyl alcohol uniformly, adding the immobilized cells, reacting for 2-16 h, centrifuging to separate the solid and liquid, taking the supernatant to obtain alkyl galactoside. The present application provides a green and efficient method for synthesizing alkyl galactoside, which is the first time to use immobilized yeast cells to synthesize glycoside with monosaccharide as glycosyl donor. The reaction system only contains alkyl glycoside and residual substrate after completion, the product types are few, easy to purify, suitable for large-scale synthesis of alkyl glycoside, and has broad application prospects.

The invention belongs to the technical field of biological functional polymer materials, and relates to a high-strength and high-toughness silk fibroin-based material as well as a preparation method and application thereof. The material has a composite cross-linked network; the composite cross-linked network is composed of a chemical cross-linked network formed among silk fibroin molecules and a physical cross-linked network formed by mutual entanglement of silk fibroin and nanofibers, the silk fibroin has a regular and uniform beta-folded crystal structure, and the nanofibers have a partial orientation structure. The preparation method comprises the following steps: adding a nanofiber dispersion liquid into a silk fibroin precursor solution with specific composition and proportion, uniformly dispersing, and carrying out visible light-induced chemical crosslinking at 25-37 DEG C to obtain silk fibroin-nanofiber composite hydrogel; and carrying out gradient ethanol treatment and vacuum drying post-treatment on the silk fibroin-nanofiber composite hydrogel to obtain the silk fibroin-nanofiber composite hydrogel. The material can be applied to the field of biomedical materials such as degradable bone nails and bone lamellas. According to the invention, the high strength and high toughness of the silk fibroin-based material are both realized.

The invention provides a drug-resistant klebsiella pneumoniae bacteriophage and application thereof. Specifically, the invention provides the klebsiella pneumoniae bacteriophage, and the preservation number of the klebsiella pneumoniae bacteriophage is CCTCC (China Center For Type Culture Collection) NO: M 20251317. The invention also provides application of the klebsiella pneumoniae bacteriophage in preparation of a preparation for specifically and efficiently splitting the drug-resistant klebsiella pneumoniae bacteriophage. The klebsiella pneumoniae bacteriophage disclosed by the invention shows specific efficient cracking capability on the drug-resistant klebsiella pneumoniae bacteriophage, can keep stable activity under the conditions of high temperature, acid-base, ultraviolet and ethanol treatment, has high safety and shows remarkable application potential.

The invention provides a preparation method of a paraffin-embedded section of decalcified bone tissue, which belongs to the field of paraffin section manufacturing and comprises the steps of fixation, decalcification, gradient concentration ethanol treatment, embedding, freezing, slicing and the like. The method for treating the glass slide is simple and low in cost, a uniform film can be formed, the section keeps good adhesiveness in the embedding and dyeing process, meanwhile, the solvent resistance is good, the problem of section falling caused by xylene dewaxing is solved, tissue loss can be remarkably reduced, and the experiment stability and repeatability are improved. In addition, a temperature gradient wax dipping method is also used, so that the permeation rate of paraffin inside and on the surface of the tissue is more uniform, a uniform and stable paraffin embedding matrix is finally formed, the slice quality is improved, and the dyeing and microscopic observation effects are clearer.

The application provides a kind of hierarchical pore SBA-15 molecular sieve and its preparation method, the preparation method includes the following steps: P123, pore expander and inorganic acid are dissolved in water to obtain a mixed solution, silicon source is added and hydrolyzed in the mixed solution to obtain a hydrolysate and mother liquor;Repeat the following operation: the mother liquor obtained in the last step is subjected to ethanol removal treatment, then P123, pore expander and inorganic acid are added and dissolved, silicon source is added and hydrolyzed again, and the solid and mother liquor are obtained again;S3: the hydrolysate obtained in S1 and S2 is combined, and the combined solid product is subjected to hydrothermal crystallization by adding the mother liquor, the crystallization product is dried and calcined to obtain the hierarchical pore SBA-15 molecular sieve.The preparation method of the application has high synthesis efficiency, low cost and less pollution, and the synthesized molecular sieve contains hierarchical pore structure and can realize cross-scale penetration.

The invention relates to the technical field of stevioside, in particular to a treatment process of low-total-glycoside mother liquor sugar in stevioside production, which comprises the following steps: collecting the low-total-glycoside mother liquor sugar after stevioside production, adjusting the pH value, adding a decolorizing agent for treatment, filtering and collecting filtrate, sequentially feeding the filtrate into anions, cations and macroporous adsorption resin columns which are connected in series, and collecting the low-total-glycoside mother liquor sugar. The collected effluent is dried, the prepared feed liquid enters a medium-low pressure chromatographic column, first, second, third and fourth eluents are sequentially adopted for elution, the collected second eluent is evaporated to dryness, then ethanol is added for crystallization treatment, drying is performed, and an RM product with the purity larger than 97% can be obtained; and evaporating the third eluent to dryness to obtain the novel sweetening agent product. And evaporating the fourth eluent to dryness, treating with ethanol, and drying to obtain an RA product with the purity of more than 97%. According to the process method, the low-total-glycoside mother liquor sugar is treated to obtain a novel sweetening agent product, an RM product with the purity larger than 97% and an RA product with the purity larger than 97%, and economic benefits are brought to enterprises.

The present invention provides a traditional Chinese medicine compound composition for treating spleen deficiency and phlegm turbidity type hypertension and its preparation method, which has the effect of improving patients' dizziness and headache symptoms and preventing the occurrence of cardiovascular and cerebrovascular diseases in hypertensive patients. To achieve the above objects, the technical solution of the present invention is as follows: the composition is as follows by mass: 20-40 parts of raw astragalus, 10-20 parts of Poria, 10-20 parts of Alisma, 10-20 parts of Pueraria, 10-20 parts of Salvia miltiorrhiza, 5-10 parts of Chuanxiong, 10-20 parts of Leonurus, and 10-20 parts of Corn Silk. The preparation method comprises the following steps: adding raw astragalus, Poria, Rhizoma Alismatis, Radix Puerariae, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, Herba Leonurus, and Stigma Corni to the above-mentioned mass fractions and adding 5 times the weight volume of water; decocting twice, each time for 1 to 2 hours; combining the decoctions; filtering and concentrating the filtrate; adding 80% ethanol by volume for treatment; allowing to stand; filtering and recovering the filtrate; and concentrating at 60°C to a relative density of 1.30 to 1.36 to obtain an extract. The extract can be prepared into a dosage form such as an ointment, oral liquid, or granule by combining the extract with a pharmaceutically acceptable carrier.

The present application provides a kind of earth dragon protein and its preparation method and application, and the method comprises the following steps: S1, mixing earth dragon powder and ethanol and post-processing, collecting solid phase; S2, mixing the solid phase and citric acid solution and extracting, collecting liquid phase; S3, mixing the liquid phase obtained in step S2 and dopamine-modified activated carbon, collecting liquid phase; S4, ultrafiltration and freeze-drying of the liquid phase obtained in step S4. The present application extracts earth dragon powder treated with ethanol and citric acid, thereby improving the full dissolution of active substances in the earth dragon powder; then, dopamine-modified activated carbon is used to adsorb small molecule inorganic substances and pigments, thereby further improving the purity and activity of earth dragon protein; finally, impurities are further removed by ultrafiltration, thereby further improving the purity and activity of earth dragon protein.

This invention relates to the field of ink surface printing technology and discloses an ink fading-resistant surface printing process, specifically: preparing an alkenylated siloxane-type polyurethane binder; compounding the alkenylated siloxane-type polyurethane binder with a hydrophilic magnetite pigment via a silanol-hydroxyl condensation reaction, and adding a composite additive and water to obtain an alkenylated magnetic ink; printing the alkenylated magnetic ink onto a mercapto-functionalized polyolefin film substrate using a gravure printing method, and fixing the ink to the substrate under ultraviolet light through an alkenyl-mercaptoclick reaction to obtain an ink fading-resistant surface-printed product. The ink adhesion of this product is >98.0%, and the ink does not fade after water treatment at 25°C for 24 hours, anhydrous ethanol treatment at 25°C for 24 hours, and 100 rubs.

The invention belongs to the technical field of development of molecular nutrition and microcapsule sustained-release particles of aquatic animals, and discloses a method for relieving fatty liver of lateolabrax japonicus and improving muscle quality based on MSTN (myostatin) gene interference, which comprises the following steps: designing a specific fragment according to an MSTN gene coding sequence of lateolabrax japonicus, inserting the specific fragment into an RNA (Ribonucleic Acid) interference vector L4440, constructing a recombinant plasmid, and transforming the recombinant plasmid into HT115 (DE3) escherichia coli; the preparation method comprises the following steps: carrying out multiplication culture in a culture medium containing double antibodies, carrying out IPTG induced expression to generate double-stranded RNA (dsRNA), collecting expressed thalli, and carrying out treatment inactivation with 75% ethanol to prepare microcapsule sustained-release particles. After the microcapsule sustained-release particles are orally delivered, the dsRNA carried by the microcapsule sustained-release particles is subjected to enzyme digestion in the body of lateolabrax japonicus to generate siRNA, and then gene silencing is induced, so that the expression level of the lateolabrax japonicus is remarkably reduced. The interference effect can adjust lipid metabolism distribution between fish body muscles and livers, reduce liver lipid deposition and promote lipid accumulation in the muscles, so that the dual purposes of relieving fatty liver and improving muscle quality are achieved.

The application relates to the field of spectral detection methods, and relates to a preparation method of a SERS (Surface Enhanced Raman Scattering) substrate with high gold nanorod loading and a use method thereof, the preparation method of the SERS substrate with high gold nanorod loading, which comprises the following steps: S1, preparing gold seeds by compounding citric acid, cetyltrimethylammonium bromide and chloroauric acid, then preparing a gold nanorod crude product through gold seed synthesis, and obtaining gold nanorods through purification; S2, obtaining a pretreated film layer by activating a polyvinylidene fluoride film through ethanol treatment and plasma cleaning; S3, loading 6-mercapto-1-hexanol and gold nanorods onto the surface of the pretreated film layer, and obtaining a finished product through drying treatment; the use method is that single-walled carbon nanotubes or graphite are placed on the SERS substrate to detect the Raman signal intensity; the SERS substrate has the advantages of high gold nanorod loading, stable loading, and fast loading speed, and can be used for enhancing the Raman signal and reducing the fluorescence background interference.

The present application relates to the technical field of blood product production, and particularly relates to a fibrinogen preparation process based on a chromatography method, comprising the following steps which are sequentially performed: treating blood plasma with ethanol and obtaining component I precipitate through pressure filtration or performing low-temperature centrifugal treatment on the blood plasma to obtain a cold precipitate; washing the precipitate multiple times with a precipitate washing solution to obtain a washed precipitate; adding a precipitate dissolving solution to the washed precipitate and filtering to obtain a filtrate; performing inactivation operation on the filtrate to obtain an inactivated filtrate; adjusting the pH and conductivity of the inactivated filtrate and then filtering; loading the product into an anion exchange chromatography column, sequentially loading a chromatography buffer, a chromatography washing solution and an eluent; and performing ultrafiltration treatment on the eluent to obtain a target product. The technical solution can solve the technical problem that the purity and yield of fibrinogen obtained by the existing preparation process are not ideal, and at the same time, the process flow is simplified, the process safety factor is improved, and the technical solution has a good application and promotion prospect.

This invention belongs to the field of biofunctional polymer materials technology, and relates to a high-strength, high-toughness silk fibroin-based material, its preparation method, and its applications. This material possesses a composite cross-linked network; the composite cross-linked network consists of a chemical cross-linked network formed between silk fibroin molecules and a physical cross-linked network formed by the entanglement of silk fibroin and nanofibers. The silk fibroin has a regular and uniform structure. β The material exhibits a folded crystalline structure with partially oriented nanofibers. It is prepared by adding a nanofiber dispersion to a silk fibroin precursor solution of specific composition and ratio, followed by uniform dispersion and then chemical cross-linking under visible light at 25–37°C to obtain a silk fibroin-nanofiber composite hydrogel. This hydrogel is then subjected to gradient ethanol treatment and vacuum drying. This material can be applied to biomedical materials such as biodegradable bone screws and plates. This invention achieves a balance between high strength and high toughness in silk fibroin-based materials.