98 results about "Ethanol treatment" patented technology

The invention relates to L-prolinamide derivative of the general formula I, a preparation method and application of the same, belonging to the chemical field. The preparation method comprises following steps of: coupling N-Boc-L-Pro to mono terminal protecting (R,R)-1,2-cyclohexanediamine of the general formula II to obtain intermediate of the general formula III, processing the obtained intermediate by using trifluoroacetic acid to remove a Boc protecting group or by using hydrazine hydrate and ethanol to remove a mono terminal protecting group to obtain the L-prolinamide derivative of the general formula I. The L-prolinamide derivative can be used as the catalyst of the intermolecular asymmetric direct Aldol reaction, has the advantages of high efficiency, high selectivity, easy operation, recycling and the like and is suitable for large-scale industrial application. The invention further discloses a method for catalyzing the intermolecular asymmetric direct Aldol reaction by using the L-prolinamide derivative.

The invention discloses a method for extracting pectin. The method comprises the following steps of: mixing dried beet pulp powder subjected to ethanol treatment with an acid solution under the condition of ultrasonic and microwave heating and carrying out extraction; regulating the pH value of an obtained extracting solution to 2-5, and then carrying out ultrafiltration concentration; and then adding anhydrous ethanol to an obtained concentrated solution for deposition, and collecting deposits to obtain the pectin. According to the method for extracting beet pectin, which is disclosed by the invention, the resource waste and the environment pollution are reduced, the comprehensive utilization of wastes obtained from beet sugar manufacture processing is realized, and the product additional value is increased. Meanwhile, the method is simple in process and easy to carry out industrialized operation by extracting the pectin by adopting existing ultrasonic and microwave machines in the present market; and the beet pectin product has the advantages of high purity, less impurity and edible safety and reliability.

The invention relates to a technology for extracting essential oil and aurantiamarin from plants, in particular to a technology for extracting essential oil and aurantiamarin from orange peel. According to the technology, a common extraction method in the prior art is improved and optimized; the steps of ethanol treatment, ultraviolet radiation and macroporous resin adsorption are boldly adopted;and various reaction parameters are effectively combined and optimized. Therefore, the output efficiencies of the essential oil and the aurantiamarin are greatly improved, and the method has a wide application prospect.

The invention relates to a process technology for extracting essential oil and neohesperidin dihydrochalcone from plants, in particular to a preparation process technology for extracting essential oil and neohesperidin dihydrochalcone from orange peels. The process technology is improved and optimized based on a conventional extraction method in the prior art, the steps of ethanol treatment, ultraviolet radiation, macroporous resin adsorption and the like are adopted audaciously, various reaction parameters are combined and optimized effectively, output efficiency of the essential oil and the neohesperidin dihydrochalcone is greatly improved, and the process technology is wide in application prospect.

The invention relates to a technology for extracting essential oil from a plant, and particularly relates to a preparation technology for extracting essential oil from an orange peel. The technology improves and optimizes the conventional extraction method in the prior art, boldly adopts the steps such as ethanol treatment, ultraviolet irradiation, salt ion aging and the like, and effectively combines and optimizes reaction parameters, thereby greatly improving the production efficiency of essential oil and having wide application prospects.

The invention discloses an application of polygonatum polysaccharide in the preparation of drugs for assisting the inhibition of colon cancer. The polygonatum polysaccharide is preferably prepared through the following steps: adopting polygonatum sibiricum as the raw material, extracting the polygonatum sibiricum with boiling water, removing the proteins, carrying out dialysis, and finally carrying out an ethanol treatment so as to obtain the polygonatum polysaccharide. The polygonatum polysaccharide can modulate the tumor micro-environment by inhibiting the tumor from generating new vessels, carrying out immunity inhibition, carrying out anti-oxidation, inhibiting the growth of colon cancer fibroblast, and the like. Thus the polygonatum polysaccharide has a very good auxiliary effect on inhibiting the colon tumor.

The invention relates to a technique for extracting pigment and essential oil from plants, particularly a preparation technique for extracting pigment and essential oil from tangerine peel. The technique improves and optimizes the conventional extraction method in the prior art, adopts the steps of ethanol treatment, ultraviolet irradiation, acetone extraction and the like, optimizes the reaction parameters, and greatly enhances the output efficiency of pigment and essential oil, thereby having wide application prospects.

The invention discloses a compound polysaccharide composition, which is obtained by taking prepared rehmannia root, medlar, sealwort and radix codonopsis as raw materials and carrying out water extraction, concentration and ethanol treatment. The invention also discloses a preparation method of the compound polysaccharide composition and an application of the compound polysaccharide composition in preparation of health care food with the function of tonifying kidney yin.

The invention belongs to the pharmaceutical field of the biochemical drugs, which relates to the preparation method of the apolipoprotein A-I, in particular to a method for preparing the apolipoprotein A-I (Apo A-I) separated from the waste human plasma F-IV component. The invention adopts the waste human plasma F-IV component to gain the 98 per cent purity through the isoelectric point precipitate, chloroform and ethanol treatment and gain the 23.5 per cent yield based on the calculation of the content of the plasma F-IV Apo A-I, and keeps the apolipoprotein A-I of the natural Apo A-I biological activity; the wet weight/g can prepare 3.1mg purified Apo A-I. The invention lays good foundation for developing the Apo A-I medicinal value and provides a preparation method of human plasma F-IV produced industrially.

The invention discloses a process and a device for preparing a nano-scale core-shell structured carbon-coated composite material, and belongs to the technical field of nano material preparation, A precursor of carbon is prepared by taking styrene and acetone as raw materials; the selected non-magnetic metal oxide is subjected to ethanol treatment and drying to obtain powder for participating in areaction; and a rotary vapor deposition system is adopted. According to the method, the thickness and the number of layers of the nano-carbon coating layer are controlled through the rotary type vapordeposition system, so that the controllable core-shell carbon-coated metal oxide is successfully prepared. The core-shell structured carbon-coated metal oxide prepared by the invention has the characteristics of uniform carbon coating layer thickness and controllable number of layers, and can realize industrial and large-scale production. The agglomeration property of the produced core-shell carbon-coated metal oxide can be improved; and the material can be widely applied to the fields of a lubricating oil additive, a catalyst, surface conductive coatings and the like, and has huge market space and commercial value prospect.

The invention discloses a colorized aglaonema tissue culture method. The method comprises the following steps: selecting healthy colorized aglaonema without insect disease, and stopping watering for several days and then sampling; rinsing by using an aqueous solution with washing powder, then flushing by running water, segmenting and treating by ethanol with concentration of 75% for 30 seconds, disinfecting by 0.1% of HgCl2 for 20 minutes, flushing by sterile water; inoculating the disinfected sterile body for culturing, illuminating for 8 hours/day to obtain an explants; laterally stabbing a growing point by a point of a knife for culturing, illuminating for 8 hours/day; cutting the bud with height greater than 15mm by taking as a single mud form, inoculating in a rooting medium for culturing, illuminating for 12 hours/day; wherein the culture condition is characterized in that light intensity is 1500-2500lx and the temperature is 25-27 DEG C, culturing for 5-6 weeks; and transplanting. By employing the method, the reproducibility is increased to 4.0, the rooting rate is increased to more than 95%, and the plantation survival rate is more than 90%.

The invention relates to a method for preparing haematochrome of red rice, which belongs to the technical field of the production of natural pigments. The method comprises the following steps of: (1) preparing an extracting agent, namely adding 0.3 percent citric acid into 15 to 45 percent food grade ethanol to adjust the pH of the ethanol to between 2.5 and 3.8, and using the mixture as the extracting agent; (2) performing extraction, namely mixing black scented rice with the extracting agent in a weight ratio of 1:1-5, performing the extraction for 1 to 3 hours at the temperature of 40 DEG C, repeating the operations for 2 to 3 times, and then combining extraction liquid; (3) performing ethanol treatment, namely concentrating the extraction liquid by using a falling film evaporator until the volume of the extraction liquid is 1/5 to 1/8 of the original volume, adding 95 percent ethanol, adjusting the ethanol content to between 65 and 80 percent, standing the mixture for 3 to 5 hoursat the temperature of between 0 and 5 DEG C, depositing the mixture in alcohol to remove impurities, then filtering the mixture by using a centrifugal machine, and collecting filtrate; and (4) performing concentration, namely concentrating the filtrate by using the falling film evaporator. The product keeps the inherent components to the utmost extent, and has high unit color scale, good stability and good water solubility.

The invention discloses a preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel. The preparation method comprises the following steps: taking hyaluronic acid and silk fibroin as raw materials, modifying the hyaluronic acid and the silk fibroin to respectively obtain hyaluronic acid-methacrylate and silk fibroin-methacrylate, mixing a hyaluronic acid-methacrylate solution and a silk fibroin-methacrylate solution according to a certain proportion to obtain a precursor solution, and constructing the hydrogel through a photo curing 3D printing technology. The degradability of the hydrogel is improved through treatment of a methanol solution or an ethanol solution. According to the invention, the excellent characteristics of silk fibroin and hyaluronic acid are combined, and the hydrogel has good bio-compatibility and adjustable degradability by controlling the mixing ratio of hyaluronic acid-methacrylate and silk fibroin-methacrylate prepolymer solutions and treating the hydrogel with methanol or ethanol.

The invention relates to a technique for extracting essential oil and pectin from plants, particularly a preparation technique for extracting essential oil and pectin from tangerine peel. The technique improves and optimizes the conventional extraction method in the prior art, boldly adopts the steps of ethanol treatment, ultraviolet irradiation, salt ion aging and the like, and effectively combines and optimizes the reaction parameters, thereby enhancing the output efficiency of essential oil and pectin and having wide application prospects.

The invention discloses a method for synthesizing 3-chloro-5-trifluoromethyl trifluoroacetophenone, which comprises the following steps of: first brominating and then chlorinating 2-aminotrifluoromethylbenzene to obtain 4-bromo-2-chloro-6-trifluoromethylaniline; diazotizing the 4-bromo-2-chloro-6-trifluoromethylaniline, and treating the diazotized 4-bromo-2-chloro-6-trifluoromethylaniline with hypophosphorous acid or ethanol to obtain a deaminated compound 1-bromo-3-chloro-5-(trifluoromethyl) benzene; and preparing a Grignard reagent from the 1-bromo-3-chloro-5-(trifluoromethyl) benzene by using magnesium chips, and carrying out a nucleophilic reaction on the Grignard reagent and an acylation reagent to obtain 3-chloro-5-trifluoromethyl trifluoroacetophenone. The initial raw material 2-aminotrifluoromethylbenzene used in the route is a common chemical with low price, the product obtained by the reaction in each step is good in purity and high in yield, the environmental pollution is relatively small, and the method is expected to be used for large-scale industrial production.

The invention discloses a new application of ethanol, namely, an application of ethanol as a treating agent in promoting plant growth, wherein when used, 2-8% of volume percent of ethanol solution is used for spraying leaves of plant seedlings growing for 2-3 weeks twice a week; continuously treating for 4-8 weeks, and observing the growth index of the plant; it is shown by experimental result that the photosynthesis ability of the plant seedlings after being treated by ethanol is strengthened, and the chlorophyll content, the net photosynthetic rate, the transpiration rate, the stomatal conductance and the intercellular CO2 concentration are increased notably; the ethanol treating agent can promote plant growth, and increase accumulation of the plant biomass (dry weight of overground part and dry weight of underground part).

The invention relates to a method for preparing agar sugar, comprising the following steps: (1) ethanol treatment: adding ethanol solution with a volume concentration of 60% to the agar, soaking the mixture for 3-4h at room temperature, filtering the mixture and washing away the ethanol by de-ionized water, adding the de-ionized water according to the weight measurement ratio of 1:40 between the agar and the de-ionized water and heating and dissolve the mixture to prepare the agar solution; (2) cellulose adsorption: taking the agar solution obtained in step (1) and adjusting temperature thereof to 80-85 DEG C, mixing the agar solution with the pretreated cellulose solution, stirring the mixture at constant temperature for 2-3h in water bath; (3) filtration, gelation and dehydration and drying: filtrating the mixed solution of agar and cellulose, then collecting filtrate, carrying out cooling and gelation, freezing the obtained gel to ice at minus 18 DEG C; after dehydration, drying, crashing and sifting out the gel, thus obtaining the agar sugar; the preparation method features easy operation, low production cost, stable production quality and the capacity of improving economic benefits of manufacturing enterprises.

The invention discloses application of salted indianmulberry root polysaccharide in preparation of functional food with the effect of helping inhibit prostate cancer. Salted indianmulberry root polysaccharide is preferably prepared by the following method: processing indianmulberry root used as a raw material with salt, extracting with boiling water, removing protein, dialyzing and carrying out ethanol treatment. The indianmulberry root polysaccharide has the function of adjusting the tumor microenvironment. The salted indianmulberry root polysaccharide can adjust the tumor microenvironment through multiple ways such as inhabitation of tumor angiogenesis, inhabitation of immunity and inhabitation of fibroblast growth of the prostate cancer, so that the salted indianmulberry root polysaccharide has a good effect of helping inhibit prostate tumors.

The present invention discloses the use of a Cistanche deserticola polysaccharide in the preparation of functional food having efficacy of supplementary for inhibiting colon cancer, wherein Cistanche deserticola polysaccharide is preferably prepared by the following method: with Cistanche deserticola as raw material, is prepared by the processes including extraction with boiling water, deproteinization, dialysis and ethanol treatment, the Cistanche deserticola polysaccharide has an effect of regulating tumor microenvironment, the Cistanche deserticola polysaccharide according to the present invention is able to regulate tumor microenvironment by many pathways of inhibiting tumor angiogenesis, immunosuppression, anti-oxidation and inhibiting the growth of colon cancer fibroblast etc., which demonstrate that it has good effect of supplementary for inhibiting colon cancer tumor.

The invention provides a method for cleaning activated alumina balls in hydrogen peroxide production, and belongs to the field of hydrogen peroxide preparation. The method comprises the steps of ethanol treatment, steam treatment, ozone treatment, calcination and vibration screening. According to the cleaning method, organic matters such as anthraquinone and anthraquinone decomposers are eluted through ethanol; substances in alumina ball holes can be promoted to be removed through vacuum and steam treatment; ozone treatment is carried out to further remove organic impurities; the internal porestructures of the alumina balls are changed by calcining, and the activity is increased; and finally, powder garbage remaining in small holes is screened through vibration, and the alumina balls withhigh activity are obtained.

The invention discloses human milk oligosaccharides and application thereof in preparation of medicines for treating or preventing NEC by relieving intestinal tract hypoxia injury. Human milk is subjected to centrifugation to remove fat to obtain whey; the whey is subjected to ultrafiltration and ethanol treatment to obtain crude oligosaccharides; the crude oligosaccharides are subjected to chromatography and mass spectrometry treatment to remove lactose-containing components; and freeze-drying is performed to obtain the purified human milk oligosaccharides (HMOs). The composition of the obtained purified human milk oligosaccharides (HMOs) is similar to that of HMOs in natural human milk. A large number of animal experiments prove that the obtained purified HMOs can effectively relieve intestinal tract hypoxia injury, in manners of promoting intestinal epithelial cell proliferation, resisting cell apoptosis and reducing cell inflammatory response, so that necrotizing enterocolitis is treated or the morbidity of necrotizing enterocolitis is reduced.

The invention discloses an organic solvent-tolerant lipase mutant and an application thereof, and belongs to the technical field of genetic engineering and enzyme engineering. An enzyme mutation library is designed and constructed based on enzyme protein structure analysis, the organic solvent tolerance enhanced lipase mutant is obtained by screening, and a pichia pastoris engineered strain whichcan efficiently express the mutant is constructed and obtained. Compared with the wild type, the organic solvent tolerance enhanced lipase mutant has significantly enhanced tolerance to organic solvents, residual vitality of the mutant is still kept to be 75% after treatment with 50% ethanol for 2 h, and the residual vitality is about 3 times that of the wild type. The lipase mutant can be appliedin preparation and processing of biodiesel and flavor esters, and has good stability, and a service life can be prolonged in the application process, so that the production cost is saved, and production efficiency is improved.

The invention belongs to the technical field of food safety, and discloses a method for preparing an adsorbent for methylene blue and organophosphorus pesticide residues in a water body by utilizing tea residues, the prepared adsorbent and application. The method comprises the following steps: crushing tea residues serving as a raw material by using a crusher, and sieving by using an 80-mesh sieveto obtain tea residue powder; sequentially treating tea residue powder with a NaOH solution, sodium chlorite, hydrogen peroxide and ethanol to obtain a crude extract, adding the crude extract into ahydrochloric acid solution with a certain concentration according to a certain solid-to-liquid ratio, reacting in a water bath kettle with a certain temperature for a certain period of time, stoppingthe reaction, washing the obtained sample to be neutral, and drying the sample to obtain the adsorbent. The adsorbent has a removal effect on organic phosphorus pesticide residues such as organic dyestuff methylene blue and chlorpyrifos in a water body. The adsorbent is easily available in raw materials, simple in preparation method and low in cost; in addition, the adsorbent can also be used forremoving other organic pollutants in a water body.

The invention discloses an application of polysaccharide of cistanche deserticola to preparation of functional foods with effects of assistance in inhibiting colon cancer. Polysaccharide of cistanche deserticola is preferably prepared by adopting a preparation method comprising the following steps: with cistanche deserticola as a raw material, extracting with boiling water, and performing deproteinization, dialysis and ethanol treatment. Polysaccharide of cistanche deserticola has a function of regulating a tumor microenvironment. Polysaccharide of cistanche deserticola provided by the invention is capable of regulating the tumor microenvironment through multiple approaches of inhibiting tumor angiogenesis, suppressing the immunity, resisting oxidation and inhibiting the colon cancer fibroblast growth, and the like so as to indicate that polysaccharide of cistanche deserticola has a good function of assistance in inhibiting the colon cancer.