6 results about "Ethanol treatment" patented technology

The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.

The application provides a kind of hierarchical pore SBA-15 molecular sieve and its preparation method, the preparation method includes the following steps: P123, pore expander and inorganic acid are dissolved in water to obtain a mixed solution, silicon source is added and hydrolyzed in the mixed solution to obtain a hydrolysate and mother liquor;Repeat the following operation: the mother liquor obtained in the last step is subjected to ethanol removal treatment, then P123, pore expander and inorganic acid are added and dissolved, silicon source is added and hydrolyzed again, and the solid and mother liquor are obtained again;S3: the hydrolysate obtained in S1 and S2 is combined, and the combined solid product is subjected to hydrothermal crystallization by adding the mother liquor, the crystallization product is dried and calcined to obtain the hierarchical pore SBA-15 molecular sieve.The preparation method of the application has high synthesis efficiency, low cost and less pollution, and the synthesized molecular sieve contains hierarchical pore structure and can realize cross-scale penetration.

The invention relates to the technical field of stevioside, in particular to a treatment process of low-total-glycoside mother liquor sugar in stevioside production, which comprises the following steps: collecting the low-total-glycoside mother liquor sugar after stevioside production, adjusting the pH value, adding a decolorizing agent for treatment, filtering and collecting filtrate, sequentially feeding the filtrate into anions, cations and macroporous adsorption resin columns which are connected in series, and collecting the low-total-glycoside mother liquor sugar. The collected effluent is dried, the prepared feed liquid enters a medium-low pressure chromatographic column, first, second, third and fourth eluents are sequentially adopted for elution, the collected second eluent is evaporated to dryness, then ethanol is added for crystallization treatment, drying is performed, and an RM product with the purity larger than 97% can be obtained; and evaporating the third eluent to dryness to obtain the novel sweetening agent product. And evaporating the fourth eluent to dryness, treating with ethanol, and drying to obtain an RA product with the purity of more than 97%. According to the process method, the low-total-glycoside mother liquor sugar is treated to obtain a novel sweetening agent product, an RM product with the purity larger than 97% and an RA product with the purity larger than 97%, and economic benefits are brought to enterprises.

This invention relates to the field of ink surface printing technology and discloses an ink fading-resistant surface printing process, specifically: preparing an alkenylated siloxane-type polyurethane binder; compounding the alkenylated siloxane-type polyurethane binder with a hydrophilic magnetite pigment via a silanol-hydroxyl condensation reaction, and adding a composite additive and water to obtain an alkenylated magnetic ink; printing the alkenylated magnetic ink onto a mercapto-functionalized polyolefin film substrate using a gravure printing method, and fixing the ink to the substrate under ultraviolet light through an alkenyl-mercaptoclick reaction to obtain an ink fading-resistant surface-printed product. The ink adhesion of this product is >98.0%, and the ink does not fade after water treatment at 25°C for 24 hours, anhydrous ethanol treatment at 25°C for 24 hours, and 100 rubs.

The invention belongs to the technical field of development of molecular nutrition and microcapsule sustained-release particles of aquatic animals, and discloses a method for relieving fatty liver of lateolabrax japonicus and improving muscle quality based on MSTN (myostatin) gene interference, which comprises the following steps: designing a specific fragment according to an MSTN gene coding sequence of lateolabrax japonicus, inserting the specific fragment into an RNA (Ribonucleic Acid) interference vector L4440, constructing a recombinant plasmid, and transforming the recombinant plasmid into HT115 (DE3) escherichia coli; the preparation method comprises the following steps: carrying out multiplication culture in a culture medium containing double antibodies, carrying out IPTG induced expression to generate double-stranded RNA (dsRNA), collecting expressed thalli, and carrying out treatment inactivation with 75% ethanol to prepare microcapsule sustained-release particles. After the microcapsule sustained-release particles are orally delivered, the dsRNA carried by the microcapsule sustained-release particles is subjected to enzyme digestion in the body of lateolabrax japonicus to generate siRNA, and then gene silencing is induced, so that the expression level of the lateolabrax japonicus is remarkably reduced. The interference effect can adjust lipid metabolism distribution between fish body muscles and livers, reduce liver lipid deposition and promote lipid accumulation in the muscles, so that the dual purposes of relieving fatty liver and improving muscle quality are achieved.

The application relates to the field of spectral detection methods, and relates to a preparation method of a SERS (Surface Enhanced Raman Scattering) substrate with high gold nanorod loading and a use method thereof, the preparation method of the SERS substrate with high gold nanorod loading, which comprises the following steps: S1, preparing gold seeds by compounding citric acid, cetyltrimethylammonium bromide and chloroauric acid, then preparing a gold nanorod crude product through gold seed synthesis, and obtaining gold nanorods through purification; S2, obtaining a pretreated film layer by activating a polyvinylidene fluoride film through ethanol treatment and plasma cleaning; S3, loading 6-mercapto-1-hexanol and gold nanorods onto the surface of the pretreated film layer, and obtaining a finished product through drying treatment; the use method is that single-walled carbon nanotubes or graphite are placed on the SERS substrate to detect the Raman signal intensity; the SERS substrate has the advantages of high gold nanorod loading, stable loading, and fast loading speed, and can be used for enhancing the Raman signal and reducing the fluorescence background interference.