Method for extracting sunflower seed meal protein based on microwave-assisted eutectic solvent system

Through the coupled extraction method of microwave-assisted eutectic solvent system and alkaline protease, the problems of low extraction efficiency and low emulsification activity of sunflower seed meal protein are solved, and efficient extraction and emulsification stability is achieved, which meets the requirements of green environmental protection and low cost.

CN119842851APending Publication Date: 2025-04-18XINJIANG UNIVERSITY
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Patent Information

Application Number
CN202510296401.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-13
Publication Date
2025-04-18

AI Technical Summary

Technical Problem

The prior art has low extraction efficiency and low emulsification activity during the extraction process of sunflower seed meal proteins, and traditional methods have problems of environmental pollution and high costs.

Method used

The sunflower seed meal protein was extracted using a microwave-assisted eutectic solvent system, and the coupling system between DES solution and alkaline protease was combined with multi-stage pulsed microwave mode and temperature cycle to induce β-sheet rearrangement, which improved extraction efficiency and emulsification activity.

Benefits of technology

It significantly improves the extraction rate of sunflower seed meal protein to more than 80%, improves its emulsification activity, reduces the use of chemical reagents, reduces production costs, and realizes the effective utilization of resources.

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Abstract

The invention discloses a method for extracting sunflower seed meal protein based on a microwave-assisted eutectic solvent system. The method comprises the following steps: pretreating sunflower seed meal to obtain degreased powder, adding the degreased powder into a DES and alkaline protease coupling system, carrying out microwave extraction, centrifugation, ethanol treatment, incubation, precipitation and washing, carrying out TiO2-coated CQDs illumination treatment, and drying to obtain protein. By optimizing microwave parameters and adopting a multi-stage pulse mode, the extraction rate is remarkably increased to 80% or above, meanwhile, the protein emulsifying activity is improved, and the application range is expanded. The DES system is environment-friendly and recoverable, the preparation process is accurate in parameter control, stable and efficient reaction is ensured, and green and efficient sunflower seed meal protein extraction is realized.
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