Determination method for isoferulic acid in Rhizoma Cimicifugae

A technology of isoferulic acid and determination method, applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills, complex pharmacopoeia operation process, etc., and achieves the effects of good repeatability, consistent accuracy and simple operation.

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • Determination method for isoferulic acid in Rhizoma Cimicifugae
  • Determination method for isoferulic acid in Rhizoma Cimicifugae
  • Determination method for isoferulic acid in Rhizoma Cimicifugae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Crush Cimicifuga through a pulverizer and pass through No. 3 sieve, about 0.5g, accurately weighed, mix evenly with 1g of quartz sand, set aside, and transfer to a pre-placed glass fiber filter. Add an appropriate amount of quartz sand to the 10ml extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, the extract was transferred to a 50ml volumetric flask, diluted to the mark with 20% methanol, centrifuged at 15000r / min for 3min, and the supernatant was taken for LC determination.

[0038] The extraction conditions (see table 1) are:

[0039] Table 1

[0040]

best practice

Extraction cell volume (ml)

10

extraction solvent

40% Methanol

pressure (psi)

1500

temperature(℃)

120

Static extraction time (min)

5

Static cycle times

4

Flushing volume (%)

60

Purge time (s)

90

[0041] A...

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Abstract

The invention discloses a determination method for isoferulic acid in Rhizoma Cimicifugae. The method comprises the following steps: step 1, crushing Rhizoma Cimicifugae and then extracting the crushed Rhizoma Cimicifugae by using an ASE extraction method so as to obtain extract; step 2, subjecting the extract obtained in the previous step to centrifugation so as to obtain supernatant; and step 3, analyzing the supernatant by using liquid chromatography. The determination method for isoferulic acid in Rhizoma Cimicifugae is simple to operate and high in detection precision.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for determining isoferulic acid in Cimicifuga. Background technique [0002] In 2010, the Pharmacopoeia recorded the method for extracting isoferulic acid in Cimicifuga: take about 0.5g of the powder of this product (through the No. 2 sieve), accurately weigh it, put it in a stoppered conical flask, add 25ml of 10% ethanol precisely, and seal it tightly. Stopper, weighed, heated to reflux for 2.5 hours, allowed to cool, weighed again, made up the lost weight with 10% ethanol, shaken well, filtered, and obtained the filtrate. Centrifuge at 15000r / min for 3min, take the supernatant, and enter the LC assay. [0003] The parameters of the liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-0.1% phosphoric acid (13:87) is used as mobile phase; detection wavelength is 316nm. [0004] The operation process of the pharmacopo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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