ASE method for extracting isoferulic acid in Rhizoma Cimicifugae

An isoferulic acid and extraction technology, which is applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills and complex pharmacopoeia operation process, and achieves the effects of good repeatability, short extraction time and simple operation.

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • ASE method for extracting isoferulic acid in Rhizoma Cimicifugae
  • ASE method for extracting isoferulic acid in Rhizoma Cimicifugae
  • ASE method for extracting isoferulic acid in Rhizoma Cimicifugae

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Experimental program
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Embodiment 1

[0023] Crush Cimicifuga through a pulverizer and pass through No. 3 sieve, about 0.5g, accurately weighed, mix evenly with 1g of quartz sand, set aside, and transfer to a pre-placed glass fiber filter. Add an appropriate amount of quartz sand to the 10ml extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, the extract was transferred to a 50ml volumetric flask, diluted to the mark with 20% methanol, centrifuged at 15000r / min for 3min, and the supernatant was taken for LC determination.

[0024] The extraction conditions (see table 1) are:

[0025] Table 1

[0026]

best practice

Extraction cell volume (ml)

10

extraction solvent

40% Methanol

pressure (psi)

1500

temperature(℃)

120

Static extraction time (min)

5

Static cycle times

4

Flushing volume (%)

60

Purge time (s)

90

[0027] A...

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Abstract

The invention discloses an ASE method for extracting isoferulic acid in Cimicifuga, which comprises the following steps: step 1: pulverize Cimicifuga, and sieve; Step 2: weigh 0.5 parts by weight of Cimicifuga powder, and Parts of quartz sand are mixed evenly for use; step 3: add an appropriate amount of quartz sand to the extraction pool with the filter membrane in advance until reaching the pool mouth of the extraction pool; cover the lid of the extraction pool for static extraction; obtain the extract after the extraction is completed, The extraction conditions are as follows: the extraction solvent is 40% methanol; the extraction temperature is 120°C; the static extraction time is 5 minutes; the washing volume is 60%; the number of static cycles is 4 times; the 40% methanol is 40% by volume of methanol. The invention provides an ASE method for extracting isoferulic acid in Cimicifuga, which is simple in operation and high in detection accuracy.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to an ASE method for extracting isoferulic acid in Cimicifuga. Background technique [0002] In 2010, the Pharmacopoeia recorded the method for extracting isoferulic acid in Cimicifuga: take about 0.5g of the powder of this product (through the No. 2 sieve), accurately weigh it, put it in a stoppered conical flask, add 25ml of 10% ethanol precisely, and seal it tightly. Stopper, weighed, heated to reflux for 2.5 hours, allowed to cool, weighed again, made up the lost weight with 10% ethanol, shaken well, filtered, and obtained the filtrate. The chromatographic conditions are: octadecylsilane bonded silica gel as filler; acetonitrile-0.1% phosphoric acid solution (13:87) as mobile phase; detection wavelength is 316nm. The number of theoretical plates should not be less than 5000 based on the peak of isoferulic acid. [0003] The operation process of the pharmacopoeia method is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/06G01N30/02G01N2030/027G01N2030/062
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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