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Application of cyclin G1 as low-dose ionizing radiation biodosimeter

A cell cycle, ionizing radiation technology, used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., to achieve high sensitivity

Inactive Publication Date: 2014-03-19
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, these studies are still in the exploratory stage, therefore, it is necessary to find new, fast and reliable low-dose ionizing radiation biological dose indicators

Method used

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  • Application of cyclin G1 as low-dose ionizing radiation biodosimeter
  • Application of cyclin G1 as low-dose ionizing radiation biodosimeter
  • Application of cyclin G1 as low-dose ionizing radiation biodosimeter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Dose-effect relationship of CCNG1 gene expression in different low doses of ionizing radiation human lymphoblastoid cells

[0019] 1. Materials: Human lymphoblastoid cells (AHH-1) were kindly provided by the Antigen Medicine Teaching and Research Office of the Second Military Medical University; TRlzol reagent and M-MLV reverse transcriptase were products of Invitrogen; SYBR Premix Ex Taq was a product of Takara.

[0020] 2. Cell culture: AHH-1 cells were cultured in RPMI1640 medium containing 10% calf serum, glutamine and 1 million U / L penicillin and streptomycin. The cells were cultured in a 37°C, 5% CO2 incubator, passaged once every 2-3 days, and the cells in the logarithmic growth phase were taken for irradiation.

[0021] 3. Irradiation: Institute of Radiation Medicine, Fudan University, Gamma cell40, 137 Cs (NORDION International Inc. CANADA), the dose rate is 0.162Gy / min, the dose is 0, 0.1, 0.2, 0.5, 0.8, 1.0Gy.

[0022] 4. Real-time PCR method to ...

Embodiment 2

[0029] Example 2: Dose-effect relationship of CCNG1 gene expression in peripheral blood lymphocytes of Balb / c mice with different low doses of ionizing radiation

[0030]1. Materials: sodium heparin; mouse lymphocyte separation fluid is a product of Daktronics Biotechnology Co., Ltd.; TRlzol reagent and M-MLV reverse transcriptase are products of Invitrogen; SYBR Premix Ex Taq is a product of Takara.

[0031] 2. Grouping and treatment of animals: 20 male Balb / c mice, weighing 20±2 g, were purchased from Shanghai Sipro-Bicai Laboratory Animal Co., Ltd. Animals were randomly divided into 4 groups, namely non-irradiated group, 0.1Gy irradiated group, 0.5Gy irradiated group and 1.0Gy irradiated group, with 5 animals in each group. Each irradiation group was irradiated in the irradiation room of the Naval Medical Research Institute, and the radioactive source was 60 Co, single irradiation, dose rate 0.5Gy / h.

[0032] 3. Separation of lymphocytes: 24 hours after the end of irradia...

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Abstract

The invention belongs to an ionizing radiation biodosimeter, and concretely relates to an application of a cyclin G1 (CCNG1) gene as the ionizing radiation biodosimeter. After low-dose ionizing radiation of a human lymphoblast and the radiation of mammals, the increase of the expression level of the mRNA of the CCNG1 gene in a peripheral blood lymphocyte is directly proportional to the applied ionizing radiation dose, there is a certain dose-effect relation, and a real-time fluorescent quantitative PCR method is adopted 24h and 48h after the radiation to realize the fast and simple quantitative detection, so the CCNG1 can be used as a biodosimeter of a low-dose ionizing radiation range, and the dose of the low-dose ionizing radiation to the human bodies and the mammals can be evaluated through a CCNG1 gene expression quantitative-analysis method.

Description

1 technical field [0001] The invention belongs to a low dose ionizing radiation biological dosimeter, in particular to the application of cell cycle protein G1 (cyclin G1, CCNG1) as a low dose range ionizing radiation biological dosimeter. 2 background technology [0002] The population and probability of low-dose ionizing radiation exposure are far greater than those of high-dose exposure events or accidents, such as natural background radiation, frequent flight activities, medical examinations, inappropriate living environments, occupational exposures, and space activities. Although low-dose ionizing radiation is not enough to cause clinically visible damage, if monitoring, hazard assessment and protective measures are not taken in time for the low-dose exposed population, it will bring long-term health hazards to the exposed personnel, and may also cause serious injury to the personnel. psychological damage, etc. As an important basis for radiation injury treatment and h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2531/113C12Q2561/113C12Q2545/101
Inventor 何颖沈先荣钱甜甜王庆蓉陈伟刘玉明蒋定文侯登勇李珂娴刘琼
Owner NAVY MEDICINE RES INST OF PLA
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