Detection method of anti-tumor activity of dendrobium huoshanense refined polysaccharide

Abstract

The invention discloses a detection method of anti-tumor activity of dendrobium huoshanense refined polysaccharide. The detection method comprises an in-vitro anti-tumor detection method and an in-vivo anti-tumor activity detection method; the inhibition rate of the dendrobium huoshanense refined polysaccharide on tumor cells is calculated through the in-vitro anti-tumor detection method; a dose-effect curve is drawn according to different concentration inhibition rates, and the half inhibitory concentration IC50 of the tumor cells is calculated; the in-vivo growth inhibition effect of the dendrobium huoshanense refined polysaccharide on a C57BL / 6J mouse in-vivo transplantable mouse lung cancer LLC tumor is detected through the in-vivo anti-tumor activity detection method; an in-vitro experiment shows that the dendrobium huoshanense refined polysaccharide has an obvious dose effect on an in-vitro anti-tumor effect; an in-vivo activity experiment shows that the dendrobium huoshanense refined polysaccharide has good anti-tumor activity and a relatively good dose-effect relation; the growth of a mouse lung cancer transplantable tumor can be remarkably inhibited and the dendrobium huoshanense refined polysaccharide has an obvious in-vivo anti-tumor effect; two experiment methods are combined so that the detection of the anti-tumor activity of the dendrobium huoshanense refined polysaccharide is more accurate.

Description

technical field [0001] The invention belongs to the key field of plant medicine technology, in particular to a method for detecting the antitumor activity of the refined polysaccharide of Dendrobium huoshanense. Background technique [0002] Malignant tumors are a serious disease that seriously endangers human health and life safety. With the destruction of the ecological environment, the rapid aging of the population, and the emergence of unhealthy lifestyles, the incidence and mortality of tumors continue to rise. It is the main cause of death of human beings and seriously endangers human health and life safety. According to data released by the International Cancer Institute of the World Health Organization in GloboCan, there were 147.07 million new cancer cases and 8.2 million deaths worldwide in 2012. Among them, the number of new cancer cases in China in 2012 was 3.065 million, and the number of deaths was 2.206 million. It is estimated that the number of new cancer c...

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Problems solved by technology

This detection method is relatively single, and cannot accurately reflect the relationship between polysaccharides and tumor inhibitory effects

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