Method for determining oleaginous microalgae harvesting time by utilizing chlorophyll fluorescence parameter Pv/Fm

Abstract

The invention discloses a method for determining the oleaginous microalgae harvesting time by utilizing the chlorophyll fluorescence parameter Pv/Fm. The method comprises the following steps: (1) carrying out conventional culture and stress induction on oleaginous microalgae, namely carrying out conventional autotrophic culture on the oleaginous microalgae; culturing the oleaginous microalgae to mid-log phase, and inducing accumulation of neutral fat under a stress condition; (2) detecting the chlorophyll fluorescence parameter Fv/Fm, namely sampling in an oil-production inducing process, and detecting Fv/Fm; (3) detecting the content of neutral fat, namely extracting algae oil by chloroform/methanol, and detecting the content of the neutral fat by means of a thin layer chromatography method and image analysis software; (4) determining the harvesting time, namely harvesting microalgae after the neutral fat in cells starts to be remarkably accumulated for 1-2 days, determining the Fv/Fm range after the neutral fat starts to be remarkably accumulated for 1-2 days to be the Fv/Fm harvesting range if the detected Fv/Fm and the neutral fat content are remarkably related, and determining the harvesting time when the algae solution Fv/Fm reaches the harvesting range in induction of neutral fat production under the same stress induction. The method is simple and quick in the chlorophyll fluorescence parameter Pv/Fm detection operation, has few interference factors, and is accurate and stable.

Description

technical field [0001] The invention belongs to the technical field of microalgae biology and bioenergy, and more specifically relates to a method for determining the harvest time of oil-producing microalgae by using the chlorophyll fluorescence parameter Fv / Fm, which is applicable to the production of microalgae bioenergy. Background technique [0002] Microalgae are prokaryotic or eukaryotic single-celled photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh or salt water and absorb light energy, water and CO transmitted by the water environment. 2 Accumulated biomass can convert light energy into chemical energy and store it in the cell in the form of organic matter such as oil or starch. As the oldest lower photosynthetic organisms, some microalgae can directly use sunlight, CO 2 And simple nutrients such as N and P grow rapidly and synthesize a large amount of oil (mainly neutral fat triglyceride) in the cells, th...

AI Technical Summary

Problems solved by technology

To assess the level of intracellular lipid or neutral lipid accumulation and thereby determine the harvest time of microalgae, the traditional gravimetric method (Bligh and Dyer, 1959, Canadian Journal of Biochemistry and Physiology, 37:911-917) involves cell disruption and lipid accumulation. Extraction takes a long time and requires at least 10-15mg of cells with a wet weight

Thin-layer chromatography (Reiser and Somerville, 1997, Journal of Bacteriology, 179:2969–2975) and GC-MS analysis (Gouveia et al., 2009, Journal of Industrial Microbiology & Biotechnology, 36:821-826) can detect For different fatty acid components of algae, the time-domain nuclear magnetic resonance system (Todt et al., 2006, Food Chemistry, 96:436-440) can conveniently quantify the oil, but the above method also needs to break the cells to extract the oil before analysis, which takes time and money. Reagent costs are higher

In order to avoid the cell disruption

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