Detection apparatus and detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence

Abstract

The invention discloses a detection apparatus and a detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence. The detection apparatus includes an excitation-emission optical structure, an excitation light source driving module, a main control module, and a fluorescent detection module and a light source reference photo-detection module, wherein the structure of the fluorescent detection module and the light source reference light detection module are same. According to the invention, an optical pulse-varying light source is employed with combination of a double-channel signal detection module, quick and accurate measurement of three optical-induced fluorescent dynamic process comprising single-turnover, multi-turnover and relaxation is achieved. By means of analysis on a fluorescent dynamic curve, a plurality detail parameters representing the photosynthesis situation, comprising functional absorption cross section, maximum photochemical quantum efficiency, electron transfer rate and the like of photosynthesis, can be obtained.

Description

technical field [0001] The invention relates to the field of photosynthesis detection devices, in particular to a phytoplankton photosynthesis detection device and method based on chlorophyll fluorescence. Background technique [0002] Phytoplankton is the primary producer of natural water bodies. Although its photosynthetic tissue accounts for less than 1% of the global total vegetation biomass, its productivity accounts for about 45% of the total plant productivity, and its photosynthetic carbon sequestration accounts for 40% of the global total carbon sequestration. [1] , is essential to maintain the normal functioning of aquatic ecosystems. In recent years, as the degree of eutrophication in my country's water quality has intensified, phytoplankton overgrowth, algal blooms and red tide disasters have occurred frequently, especially since the 1970s, the frequency of algal blooms and red tides has increased by three times every decade The continuous rise has seriously enda...

AI Technical Summary

Problems solved by technology

However, the existing fluorescence technology has obvious shortcomings: ① there are multiple light sources, and the optical structure is complex; ② the detection sensitivity is low; ③ the regulation of photosynthesis energy flow process is not flexible enough, and the photosynthesis diagnosis is not comprehensive enough.

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