Detection apparatus and detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence

A chlorophyll fluorescence and phytoplankton technology, which is applied in the field of photosynthesis detection devices, can solve the problems of low detection sensitivity, multiple light sources, complex optical structures, etc., achieve uniform illumination, overcome the interference of fluorescence detection, and achieve the effects of high-sensitivity detection

Inactive Publication Date: 2015-08-05
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, the existing fluorescence technology has obvious shortcomings: ① there are multiple light sources, and the optical structure is complex; ② the detection

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  • Detection apparatus and detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence
  • Detection apparatus and detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence

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Embodiment Construction

[0023] The phytoplankton photosynthesis detection device based on chlorophyll fluorescence comprises an excitation-emission optical structure 100, an excitation light source drive module 200, a main control module 500, a fluorescence detection module 300 with the same structure and a light source reference light detection module 400, wherein:

[0024] The excitation-emission optical structure 100 includes a sample cell 13 and an excitation light source 10 composed of a plurality of LED chips arranged in an array. The excitation light source 10 is arranged outside the front of the sample cell 13, and the front end of the light path of the excitation light source 10 is sequentially provided with filters facing the sample cell. 11. Quartz light guide column 12, outside the sample cell 13 and the side perpendicular to the front are provided with focusing lens 15, optical filter 16, interference filter 17, photomultiplier tube PMT18 in sequence, and the outgoing light of excitation l...

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Abstract

The invention discloses a detection apparatus and a detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence. The detection apparatus includes an excitation-emission optical structure, an excitation light source driving module, a main control module, and a fluorescent detection module and a light source reference photo-detection module, wherein the structure of the fluorescent detection module and the light source reference light detection module are same. According to the invention, an optical pulse-varying light source is employed with combination of a double-channel signal detection module, quick and accurate measurement of three optical-induced fluorescent dynamic process comprising single-turnover, multi-turnover and relaxation is achieved. By means of analysis on a fluorescent dynamic curve, a plurality detail parameters representing the photosynthesis situation, comprising functional absorption cross section, maximum photochemical quantum efficiency, electron transfer rate and the like of photosynthesis, can be obtained.

Description

technical field [0001] The invention relates to the field of photosynthesis detection devices, in particular to a phytoplankton photosynthesis detection device and method based on chlorophyll fluorescence. Background technique [0002] Phytoplankton is the primary producer of natural water bodies. Although its photosynthetic tissue accounts for less than 1% of the global total vegetation biomass, its productivity accounts for about 45% of the total plant productivity, and its photosynthetic carbon sequestration accounts for 40% of the global total carbon sequestration. [1] , is essential to maintain the normal functioning of aquatic ecosystems. In recent years, as the degree of eutrophication in my country's water quality has intensified, phytoplankton overgrowth, algal blooms and red tide disasters have occurred frequently, especially since the 1970s, the frequency of algal blooms and red tides has increased by three times every decade The continuous rise has seriously enda...

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Application Information

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IPC IPC(8): G01N21/64G01N33/58
Inventor 殷高方赵南京胡丽覃志松张小玲石朝毅肖雪马明俊段静波方丽刘建国刘文清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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