Method for rapidly and quantitatively calculating inherent drought resistance of plants

A quantitative calculation and anti-drought technology, which is applied in agricultural engineering, crop information detection, and drought-resistant seed selection. It can solve the problems of long measurement time and slow response to plant water changes, and achieve fast speed, timely response to fluorescence information, and high accuracy. high effect

Active Publication Date: 2014-08-27
JIANGSU UNIV
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  • Abstract
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Problems solved by technology

[0008] For the current patent "A Method for Quantitatively Detecting Drought Resistance of Plants" (Patent No.: 201310701096.X), it mainly reflects the drought resistance of plants from the perspective of relative tension of leaves; using LCR tester (HI...

Method used

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  • Method for rapidly and quantitatively calculating inherent drought resistance of plants
  • Method for rapidly and quantitatively calculating inherent drought resistance of plants

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Experimental program
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Embodiment 1

[0031] In the first step, before the experiment, fresh branches of syringa plants with relatively consistent growth were picked on the campus of Jiangsu University, and the base of the branches of the plants was wrapped with a damp cloth to slow down the water loss.

[0032] The second step is to quickly return to the laboratory. After cleaning the dust on the surface of the leaves, pick 6 fresh leaves of the mulberry tree of the same size and put them in a basin filled with water to soak for 30 minutes.

[0033] Step 3: After soaking the leaves for 30 minutes, they become saturated with water. Take out the saturated leaves after soaking, quickly and gently absorb the water on the surface of the leaves with a dry towel and facial tissue, and then place them on a dry and ventilated table.

[0034] The fourth step is to take the above-mentioned leaves and use the IMAGING-PAM modulated chlorophyll fluorescence meter to measure the initial fluorescence value (Fo) and PSⅡ maximum ph...

Embodiment 2

[0040] In the first step, before the experiment, 6 leaves of mulberry trees with relatively uniform growth were picked on the campus of Jiangsu University and put into fresh-keeping bags.

[0041] The second step is to quickly return to the laboratory, and put the picked fresh leaves into a basin filled with water to soak for 30 minutes.

[0042] The third step is to take out the soaked leaves after 30 minutes, dry the water on the surface of the leaves with a dry towel and tissue paper, etc., and place them on a dry and ventilated desktop.

[0043] The 4th step, take above-mentioned leaf and measure the initial fluorescence value (Fo) and PSⅡ maximum photochemical quantum yield (Fv / Fm) of mulberry leaf when measuring 0 level fluorescence with IMAGING-PAM modulation type chlorophyll fluorescence meter, repeat measurement 3 times, average value is taken as The measured value at this moment.

[0044] In the fifth step, the fourth step is repeated every hour to measure the chlor...

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Abstract

The invention discloses a method for rapidly and quantitatively calculating inherent drought resistance of plants. The method comprises the following steps: cleaning to-be-measured plant leaves, soaking the plant leaves in water, taking out the leaves after 30 minutes, and absorbing water on the surface; measuring minimal fluorescence (Fo) and PSII maximum photochemical quantum yield (Fv/Fm) of the leaves during 0 level fluorescence by using an IMAGING-PAM modulation type chlorophyll fluorescence spectrometer, and repeating for three times; then allowing the leaves to lose water, and repeating the operation every an hour; taking the measurement result during waterlogged 0 hour as reference, calculating relative Fo and relative Fv/Fm at each measurement moment, and respectively adding to obtain the accumulated relative Fo (TRSF) and accumulated relative Fv/Fm (TRPF) in the previous five hours after waterlogging; and comparing the numerical values of the TRSF and TRPF, and quantifying the inherent drought resistance of different plants. The method has the advantages of high accuracy, simplicity and rapidness in operation, high speed and the like and can be used for breeding drought-resistant strains.

Description

technical field [0001] The invention relates to a method for quickly and quantitatively calculating the inherent drought-resistant ability of plants under a standard dehydration mode, and belongs to the technical fields of drought-resistant seed selection, agricultural engineering and crop information detection. Background technique [0002] The growth and development of plants is inseparable from photosynthesis. Photosynthesis is the basis of all material metabolism and energy metabolism in the biological world, including a series of complex processes of photophysics, photochemistry and biochemical transformation. The original reaction of photosynthesis will absorb light energy In the process of transmission and conversion into electrical energy, part of the light energy loss is released in the form of longer fluorescence. [0003] There is a very close relationship between chlorophyll fluorescence and photosynthesis under natural conditions. On the one hand, when plants a...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 吴沿友张明明邢德科于睿赵宽周贵尧乔韡轶
Owner JIANGSU UNIV
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