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A Rapid Quantitative Method for Calculating Intrinsic Drought Resistance of Plants

A quantitative calculation, drought-resistant technology, applied in agricultural engineering and crop information detection, drought-resistant seed selection, can solve problems such as long measurement time, slow response to plant water changes, and achieve fast speed, timely response to fluorescence information, and easy operation. quick effect

Active Publication Date: 2016-06-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] For the current patent "A Method for Quantitatively Detecting Drought Resistance of Plants" (Patent No.: 201310701096.X), it mainly reflects the drought resistance of plants from the perspective of relative tension of leaves; using LCR tester (HIOKI3532-50, Japan Hiki) When the water potential meter (WaterPotentialSystem, WESCOR, USA) measures the tension of plant leaves, due to the need for stability and balance, the measurement time is longer and the response to plant water changes is slower.

Method used

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  • A Rapid Quantitative Method for Calculating Intrinsic Drought Resistance of Plants
  • A Rapid Quantitative Method for Calculating Intrinsic Drought Resistance of Plants
  • A Rapid Quantitative Method for Calculating Intrinsic Drought Resistance of Plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In the first step, before the experiment, fresh branches of syringa plants with relatively consistent growth were picked on the campus of Jiangsu University, and the base of the branches of the plants was wrapped with a damp cloth to slow down the water loss.

[0033] The second step is to quickly return to the laboratory. After cleaning the dust on the surface of the leaves, pick 6 fresh leaves of the mulberry tree of the same size and put them in a basin filled with water to soak for 30 minutes.

[0034] Step 3: After soaking the leaves for 30 minutes, they become saturated with water. Take out the saturated leaves after soaking, quickly and gently absorb the water on the surface of the leaves with a dry towel and facial tissue, and then place them on a dry and ventilated table.

[0035] The fourth step is to take the above-mentioned saturated leaves treated in step three and use the IMAGING-PAM modulated chlorophyll fluorescence meter to measure the initial fluorescen...

Embodiment 2

[0041] In the first step, before the experiment, 6 leaves of mulberry trees with relatively uniform growth were picked on the campus of Jiangsu University and put into fresh-keeping bags.

[0042] The second step is to quickly return to the laboratory, and put the picked fresh leaves into a basin filled with water to soak for 30 minutes.

[0043] The third step is to take out the soaked leaves after 30 minutes, dry the water on the surface of the leaves with a dry towel and tissue paper, etc., and place them on a dry and ventilated desktop.

[0044] The 4th step, get above-mentioned initial fluorescence value (Fo) and PSⅡ maximum photochemical quantum yield value (Fv / Fm) of mulberry leaf when measuring 0 level fluorescence with the IMAGING-PAM modulated chlorophyll fluorescence meter of the above-mentioned saturated leaves treated by step 3, The measurement was repeated 3 times, and the average value was taken as the measured value at this moment.

[0045] In the fifth step, ...

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Abstract

The invention discloses a method for quickly and quantitatively calculating the inherent drought resistance ability of plants, which comprises the following steps: cleaning the leaves of the plants to be tested and soaking them in water, taking out the leaves after 30 minutes and sucking up the water on the surface; using IMAGING-PAM The initial fluorescence (Fo) and PSII maximum photochemical quantum yield (Fv / Fm) of leaves at 0 level of fluorescence were measured by a modulated chlorophyll fluorescence instrument, and repeated 3 times; then, the above leaves were dehydrated, and the above operation was repeated every 1 hour. Taking the measured results of water saturation for 0 hours as a reference, calculate the relative Fo and relative Fv / Fm at each measurement time, and add them up respectively to obtain the cumulative relative Fo (T RSF ) and cumulative relative Fv / Fm (T RPF ). Compare T RSF and T RPF Numerical magnitude, quantifying the inherent drought resistance of different plants 。 The invention has the advantages of high precision, simple and convenient operation, high speed and the like, and can be used for breeding of drought-resistant varieties.

Description

technical field [0001] The invention relates to a method for quickly and quantitatively calculating the inherent drought-resistant ability of plants under a standard dehydration mode, and belongs to the technical fields of drought-resistant seed selection, agricultural engineering and crop information detection. Background technique [0002] The growth and development of plants is inseparable from photosynthesis. Photosynthesis is the basis of all material metabolism and energy metabolism in the biological world, including a series of complex processes of photophysics, photochemistry and biochemical transformation. The original reaction of photosynthesis will absorb light energy In the process of transmission and conversion into electrical energy, part of the light energy loss is released in the form of longer fluorescence. [0003] There is a very close relationship between chlorophyll fluorescence and photosynthesis under natural conditions. On the one hand, when plants a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 吴沿友张明明邢德科于睿赵宽周贵尧乔韡轶
Owner JIANGSU UNIV
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