Method or measuring stress of heavy metals on plants by using plant micro-domain chlorophyll fluorescence method

A technology for chlorophyll fluorescence and heavy metals, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of high experimental conditions, single excitation wavelength, improper operation, etc., and achieve simple measurement process, high optical power density, and external influence small effect

Inactive Publication Date: 2015-04-29
SHANDONG NORMAL UNIV
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Problems solved by technology

[0004] At present, the existing research results use the He-Cd laser excitation wavelength of 325nm to obtain three plant fluorescence bands of 450nm, 690nm and 735nm, and use the ratio of F450 / (F690+F735) to directly reflect the degree of stress and damage to plants by heavy metals; The He-Cd laser is used as the excitation light source to excite the chlorophyll fluorescence of plants, and at the same time obtain 3 plant fluorescence bands, and use the F450 / (F690+F735) ratio to quantitatively reflect the degree of heavy metal stress on plants, which was applied by the applicant in 2011 Patent No. 201110198388.7 discloses relevant technical content, but the He-Cd laser excitation wavelength is a single 325nm, although three plant fluorescence bands of 450nm, 690nm and 735nm can be obtained at the same time, but the experimental conditions are relatively high. Leaf measurement is relatively accurate. For solanaceous leaves, broad-leaved tree leaves, etc., improper operation will easily lead to large errors.

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  • Method or measuring stress of heavy metals on plants by using plant micro-domain chlorophyll fluorescence method

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Embodiment 1

[0014] Example 1: In this example, through cultivation experiments, pot experiments, and comparative experiments, under the condition of not destroying the growth of plants, using a xenon lamp as a laser source, systematically implement laser-induced plant growth in different plants, different heavy metals, and different soils. Spectral characteristics, quantitative methods and influencing factors of fluorescence, the specific implementation process can be found in figure 1 The present embodiment relates to the determination of the spectral characteristics and instrument operating conditions of the xenon lamp excitation light source. Its xenon lamp laser source is at a wavelength of 325nm., and the slit width is 0.05mm. Photoluminescence intensities were recorded at wavelengths from 350 to 800 nm.

Embodiment 2

[0015] Embodiment 2: This embodiment relates to the spectral characteristic parameters of different plant leaves under different heavy metal stresses. The Hoagland nutrient solution is used as the hydroponic nutrient solution, and 0-10.0mg.L is added respectively -1 Cu 2+ (CuSO 4 ), 0-1.0mg.L -1 Cd 2+ (CdCl 2 ), 0-10.0mg.L -1 Zn 2+ (ZnSO 4 ), 0-10.0mg.L -1 of Pb 2+ (PbCl 2 ), simulated heavy metal stress, planted different plants, cultivated in greenhouse (25°C), monitored the pH of the nutrient solution, measured the characteristic parameters of fluorescence spectra of different plants and plant leaves under different heavy metal stress, determined the instrument conditions, the operation steps of the method, and the quantitative Methods: Under the stress of different concentrations of Zn, Cu, Pb or Cd, the fluorescence intensity of plants appeared peaks at 450nm, 690nm and 735nm. The peak heights at 690nm and 750nm decreased with the concentration of heavy metal st...

Embodiment 3

[0016] Example 3: This example involves the use of three fluorescence bands to calculate the stress of heavy metals in soil. In order to find the relationship between the fluorescence intensity and the absorption of heavy metals by plant leaves, the peak heights of the three peaks are used to calculate F450 / F690 , the ratios of F450 / F735, F690 / F735 and F450 / (F690+F735); the three ratios of F450 / F690, F450 / F735 and F450 / (F690+F735) all increased with the increase of heavy metal stress concentration; such as, F450 / F690 increases with the concentration of Cd; F450 / F735 increases with the concentration of Cu; only the ratio of F450 / (F690+F735) increases with the increase of heavy metal stress concentration in the range of leaves and heavy metals used in the experiment ; Therefore, A=F450 / (F690+F735) reflects the degree of plant stress by heavy metals, the greater the value of A, the more serious the stress of heavy metals.

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Abstract

The invention belongs to the technical field of heavy metal pollution analysis and relates to a method or measuring stress of heavy metals on plants by using a plant micro-domain chlorophyll fluorescence method. The method comprises the following steps: putting plant leaves and quartz glass sheets in a shading box together, taking the representative part of to-be-measured plant leaves, regulating the temperature of a measuring environment to be 20 DEG C, selecting an xenon lamp as a plant fluorescence direct excitation source under shading or infrared conditions, adopting a parallel light source, and recording the photoluminescence intensity; and finally, determining the expression amount of plant leaves stressed or damaged by heavy metals. The method is simple in measurement process, reliable in design principle, convenient to use and operate, high in measurement accuracy, wide in application range and friendly in measurement environment.

Description

Technical field: [0001] The invention belongs to the technical field of heavy metal pollution analysis, and relates to a method for quickly detecting the harm of heavy metals in soil to plants, in particular to a method for quickly detecting the stress of heavy metals in soil to plants by using the micro-field chlorophyll fluorescence method of fresh leaves of plants. Background technique: [0002] With the mining and utilization of metal mineral resources, heavy metal elements enter farmland through air and sewage irrigation, seriously affecting crop yield and quality of agricultural products, and endangering human health. Therefore, no matter from the perspective of food safety and soil environmental management, or from the perspective of contaminated soil remediation, research on the migration and transformation of heavy metals in soil-plant systems is essential. The traditional research method is to complete with the help of a large number of pot experiments and tedious ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 成杰民胡光鲁
Owner SHANDONG NORMAL UNIV
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