Plant chlorophyll fluorescence detecting device for equalizing irradiation

A technology of chlorophyll fluorescence and detection devices, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., and can solve the problems that the leaf illumination imaging cannot be performed, and the light source cannot reach the plants uniformly.

Inactive Publication Date: 2013-04-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The light source of the detection device cannot evenly reach all parts of the plant, and can only detect the fluores...

Method used

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  • Plant chlorophyll fluorescence detecting device for equalizing irradiation
  • Plant chlorophyll fluorescence detecting device for equalizing irradiation
  • Plant chlorophyll fluorescence detecting device for equalizing irradiation

Examples

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Embodiment 1

[0027] Such as figure 1 As shown, this embodiment discloses a chlorophyll fluorescence detection device with a cubic structure and uniform illumination.

[0028] The uniformly illuminated chlorophyll fluorescence detection device in this embodiment consists of a light source 1, a camera 2, a filter 3 and a mirror 4, and the light source 1, camera 2 and filter 3 are located on the top of the detection device.

[0029] The light source 1 is composed of at least two identical LED light sources, and the light source 1 is evenly arranged around the camera 2 to provide uniform illumination for fluorescent imaging. It is required to adjust the luminous characteristics of the light source 1. In the actual measurement, the light source 1 can emit blue light or red light, and the waveform of the driving current is modulated by the driving circuit. The light source 1 can provide three levels of illumination, which are measuring light, Saturated pulsed light and actinic light.

[0030] ...

Embodiment 2

[0037] Such as figure 2 As shown, this embodiment discloses a chlorophyll fluorescence detection device with a cube structure at the bottom and a terraced top with uniform illumination.

[0038] The uniformly illuminated chlorophyll fluorescence detection device in this embodiment consists of a light source 1, a camera 2, a filter 3 and a reflector 4, and the light source 1, camera 2 and filter 3 are all located at the top of the trapezoidal shape of the detection device.

[0039] The settings of the light source 1, the camera 2 and the optical filter 3 in this embodiment are the same as those in the first embodiment.

[0040] Such as Figure 4 As shown, in the present embodiment, reflective mirror 4 is made up of four rectangular reflective mirrors 43 and four trapezoidal reflectors 42, and four rectangular reflective mirrors 43 are arranged around the sample to be measured, and the bottoms of four trapezoidal reflective mirrors 42 are connected with four trapezoidal reflec...

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Abstract

The invention discloses a plant chlorophyll fluorescence detecting device for equalizing irradiation. The plant chlorophyll fluorescence detecting device for equalizing irradiation comprises a light source, a camera, a light filler and a light reflection mirror, wherein the light source is used for transmitting detection light for a to-be-detected sample; the camera is used for collecting light rays from the to-be-detected sample; the light filler is arranged between the camera and the to-be-detected sample; and the light reflection mirror is used for reflecting detecting light from the light source to the to-be-detected sample. The plant chlorophyll fluorescence detecting device for equalizing irradiation provided by the invention can provide three kinds of irradiation including measurement light, saturation pulse light and actinic light; the light emitted by the light source is reflected by the light reflection mirror; and a space with equal irradiation is formed in the device, so that irradiation imaging can be carried out on laminas of plants with different heights.

Description

technical field [0001] The invention relates to a fluorescence detection device, in particular to a plant chlorophyll fluorescence detection device with uniform illumination. Background technique [0002] Photosynthesis is one of the most important chemical reactions on the earth. It uses sunlight to split water to release most of the oxygen needed on the earth, and at the same time fixes carbon dioxide in the atmosphere to provide glucose for metabolism. In the light reaction process of photosynthesis, the absorbed light energy is transferred. In the process of converting light energy into electrical energy, part of the light energy loss is released in the form of longer wavelength fluorescence. Chlorophyll fluorescence has a very close relationship with photosynthesis. Many changes in photosynthesis process can be reflected by chlorophyll fluorescence. Chlorophyll fluorescence measurement has the characteristics of fast, reliable, sensitive, and no damage to samples. Thes...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 李海峰王恒刘旭徐良
Owner ZHEJIANG UNIV
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