Plant physiology monitoring method based on chlorophyll fluorescence and multispectral image

A technology of chlorophyll fluorescence and multi-spectral images, which is applied in the field of plant detection, can solve problems such as the inability to comprehensively distinguish plant physiological conditions, and achieve the effect of improving accuracy

Active Publication Date: 2016-05-04
ZHEJIANG UNIV
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Problems solved by technology

[0006] However, the above-mentioned patent documents all use a single chlorophyll fluorescence parameter to analyze and measure the state of the plant, wh

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  • Plant physiology monitoring method based on chlorophyll fluorescence and multispectral image
  • Plant physiology monitoring method based on chlorophyll fluorescence and multispectral image
  • Plant physiology monitoring method based on chlorophyll fluorescence and multispectral image

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Embodiment Construction

[0039] This embodiment takes the common crop cucumber as an example, such as figure 1 Shown, plant physiology monitoring method comprises the following steps:

[0040] (1) After sufficient dark adaptation (lighting conditions less than 1 lux, for more than 30 minutes), the modulated chlorophyll fluorescence measurement is carried out;

[0041] First turn on the measurement light (0.1μmolphotonsm -2 the s -1 left and right), measure the minimum fluorescence F of dark-adapted leaves 0, followed by a saturated light pulse (greater than 6000 μmolphotonsm -2 the s -1 ), to temporarily saturate the photoreaction process, and then use the measurement light to measure the maximum fluorescence Fm of the leaf. Turn on the actinic light (generally 500-3000μmolphotonsm -2 the s -1 ) Simulate the ambient light illumination to make the measured material carry out photosynthesis. When the photosynthesis of the material is stable, use the measurement light to measure the steady-state f...

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Abstract

The invention discloses a plant physiology monitoring method based on chlorophyll fluorescence and a multispectral image. The method comprises the following steps that modulated type chlorophyll fluorescence detection is performed on plants to be detected, modulated type chlorophyll fluorescence characteristic parameter diagrams are acquired, and chlorophyll fluorescence parameters related to the plant physiology condition are preset according to the characteristic parameter diagrams; multispectral image measurement is performed on the plants to be detected, and a spectral relative reflectivity parameter diagram of the reflection intensity at the waveband of 540-560 nm to the reflection intensity at the waveband of 500-515 nm is obtained; chlorophyll fluorescence parameter values in a plant area and the spectral relative reflectivity parameter diagram are counted; the plant physiology condition is judged by combining a distribution curve, acquired in the step 3, of the chlorophyll fluorescence parameter values and a distribution curve, acquired in the step 3, of spectral reflectivity parameter values. According to the method, nondestructive testing is performed on the plant physiology by combining a multispectral imaging technology of visible light with a chlorophyll fluorescence detection technology, the method is simple, judging is accurate, and the reliability is high.

Description

technical field [0001] The invention relates to the technical field of plant detection, in particular to a plant physiological monitoring method based on chlorophyll fluorescence and multispectral images. Background technique [0002] The non-destructive acquisition and identification of plant physiological status information is of great significance to the fields of breeding and automatic production. The current nondestructive acquisition of plant physiological status information is mostly based on a single detection technology, such as visible light multispectral images, near-infrared images, and terahertz detection technologies, which often have certain effects on specific plant physiological status detection. [0003] With the development of photosynthetic theory, the analysis technology of chlorophyll fluorescence dynamics, especially fast chlorophyll fluorescence induction kinetics is becoming more and more perfect. Due to the advantages of rapidity and non-destructiv...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/25
CPCG01N21/25G01N21/64
Inventor 李海峰王恒夏晓剑戴连奎徐赛龙张兰徐良刘旭
Owner ZHEJIANG UNIV
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