N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield as well as preparation method and application of N-CQDs

A technology of fluorescence quantum yield and carbon quantum dots, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of complicated preparation process and low fluorescence quantum yield, and achieve low cytotoxicity and biophase Good capacitance and small molecular weight

Inactive Publication Date: 2016-07-27
NANJING UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

[0006] Aiming at the shortcomings of the existing nitrogen-doped carbon quantum dots with low fluorescence quantum yield and complicated preparation process, the present inventio

Method used

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  • N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield as well as preparation method and application of N-CQDs
  • N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield as well as preparation method and application of N-CQDs
  • N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield as well as preparation method and application of N-CQDs

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Embodiment 1

[0040] A method for preparing nitrogen-doped carbon quantum dots of the present invention, comprising the steps of:

[0041] Step 1. Weigh 2.673g of alanine (Ala) into a 50mL clean beaker, add 30.0mL of ultrapure water, put it into an ultrasonic instrument for 2 minutes, and slowly add 500μL of ethylenediamine (EDA), Ala and EDA while stirring The molar ratio is 4:1;

[0042] Step 2. Transfer the above mixed solution to the lining of a 50mL polytetrafluoroethylene reactor and install a sealed steel sleeve, place it in a blast heating box, gradually heat it to 200°C and keep the temperature constant for 6 hours;

[0043] Step 3, after the reaction finishes, cool down to room temperature naturally to obtain a tan transparent solution;

[0044] Step 4, the solution is dialyzed in ultrapure water for 24 hours with a dialysis bag with a molecular weight cut-off MWCO=1000Da to remove unreacted precursors;

[0045] Step 5. The dialyzed carbon quantum dot solution is rotatably evapo...

Embodiment 2

[0048] A method for preparing nitrogen-doped carbon quantum dots, the only difference between Example 2 and Example 1 is that the amount of EDA in step 1 is 2.0mL, and the molar ratio of Ala to EDA is 4:4, and the prepared N-CQDs The fluorescence quantum yield is 33.3%.

Embodiment 3

[0050] A method for preparing nitrogen-doped carbon quantum dots, the only difference between embodiment 3 and embodiment 2 is that the hydrothermal temperature in step 2 is 150°C, and the fluorescence quantum yield of the prepared N-CQDs is 8.64%.

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Abstract

The invention discloses N-CQDs (nitrogen-doped carbon quantum dots) with high fluorescence quantum yield and a preparation method of the N-CQDs. Alanine is taken as a carbon source, ethanediamine is taken as a surface passivator, and the N-doped carbon quantum dots with high fluorescence quantum yield is successfully prepared with a simple one-step hydrothermal method. Compared with carbon quantum dots synthesized with other protein or amino acid as a carbon source, the nitrogen content of the prepared N-CQDs can reach 61.1%, the average fluorescence lifetime is 4.43 ns, the highest fluorescence quantum yield can reach 46.2% and approaches the fluorescence quantum yield of the carbon quantum dots prepared with a laser ablation method and an electric arc method, and the N-doped carbon quantum dots have low cytotoxicity and excellent biocompatibility and has wide application value in biosensing and bioimaging. The invention further discloses a high quenching effect of NADH (nicotinamide adenine dinucleotide) on fluorescence of the N-CQDs. On the basis that NADH has high quenching effect on fluorescence of the N-CQDs, high-sensitivity fluorescence biosensing for detecting NADH is established, the linear detection range is as low as 80 mu M, and the limit of detection is 25.1 nM.

Description

technical field [0001] The invention belongs to the technical field of preparation of fluorescent carbon nanomaterials, and relates to a nitrogen-doped carbon quantum dot with a high fluorescence quantum yield, a preparation method and an application thereof, in particular to a kind of carbon source with alanine as a carbon source and ethylenediamine as a carbon source. The passivating agent is a method for preparing nitrogen-doped carbon quantum dots by a one-step hydrothermal method. The specific applications include ultra-sensitive detection of nicotinamide adenine dinucleotide and fluorescence imaging of living cells. Background technique [0002] Carbon quantum dots can be passivated by organic surfaces to improve their fluorescence properties. Nitrogen atoms can improve the surface defects of carbon quantum dots and enhance their fluorescence intensity and fluorescence quantum yield, so the preparation of nitrogen-doped carbon quantum dots (N-CQDs) has become a researc...

Claims

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Application Information

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IPC IPC(8): C09K11/65G01N21/64
CPCC09K11/65G01N21/6428G01N2021/6439
Inventor 单丹朱容慧牛文军华艳李怡萱宗丽萍
Owner NANJING UNIV OF SCI & TECH
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