Wide-view-field chromatography hyperspectral microscopic imaging method and device based on space-time focusing

Abstract

The invention discloses a wide-view-field chromatography hyperspectral microscopic imaging method and device based on space-time focusing, and belongs to the technical field of microscopic spectral imaging and analytical chemistry. According to the method, ultra-short pulse lasers are generated through an ultra-short pulse laser source, focusing lines are generated in a sample through space-time focusing, excited fluorescence is collected, stray light is filtered out through con-focal optical slits, spectral information (x, lambda), of the sample is acquired through fluorescence spectral information collecting, and finally sample (x, lambda, y, z, t) five-dimensional information is acquired through three-dimensional space scanning and delayed scanning. The device comprises the ultra-shortpulse laser source, a light beam conversion system, a line scanning system based on space-time focusing, an optical microscopic system and a filtering and synchronous spectrum con-focal detection system. Acquisition of the spectral information in the filtering and synchronous spectrum con-focal detection system is synchronous with line scanning trigger signals in the line scanning system in combination with the space-time focusing technology. The method and device have the advantages of being wide in view field, high in space resolution, high in time resolution, high in spectral resolution andthe like.

Description

technical field [0001] The invention relates to a wide field of view tomographic hyperspectral microscopic imaging method and device based on spatiotemporal focusing, and belongs to the technical fields of microspectral imaging and analytical chemistry. Background technique [0002] Hyperspectral Microscopy (Hyperspectral Microscopy) has important applications in the field of biomedical research, especially in the fields of clinical disease diagnosis, intraoperative image navigation and other fields, which have attracted more and more attention. Using hyperspectral microscopic imaging technology to obtain spatially resolved spectral information can provide information on physiological parameters, morphology and components of biological tissues for disease diagnosis. At present, non-invasive detection of various cancers can be realized by using hyperspectral microscopic imaging technology. [0003] In essence, hyperspectral microscopic imaging technology is a technology to o...

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Problems solved by technology

On the other hand, although nonlinear optical microscopy using surface excitation avoids the speed bottleneck caused by point-by-point scanning, the excited signal has serious crosstalk after being scattered by tissue, which is not suitable for scattering tissue spectral imaging.

Ordinary nonlinear optical microscopy using line-scanning method can compromise between imaging speed and suppression of scattering effects, but this method is not an ideal choice because of the lower axial resolution obtained by point-scanning method

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