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Multi-angle based selective light-sheet illumination microscopy imaging reconstruction method

A technology of microscopic imaging and light sheeting, applied in 3D modeling, image enhancement, image analysis, etc., can solve the problems of low axial resolution of volume data, uneven grayscale of collected data, and blurred stripe artifacts. , to improve image quality, improve axial and lateral resolution, reduce striping artifacts and scatter blur

Active Publication Date: 2017-02-22
INST OF AUTOMATION CHINESE ACAD OF SCI
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Problems solved by technology

[0003] However, during the imaging process of SPIM, due to the difference in the internal structure of the sample, the scattering and absorption of light are different, which will lead to a strong attenuation of the light, and finally there will be stripe artifacts and scattering blur. Uneven degree
In addition, since the thickness of the illumination light sheet and the spacing of the light sheets of SPIM are much larger than the resolution of the acquisition image plane, this will cause the axial resolution of the volume data to be much lower than the lateral resolution

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[0034] The reconstruction method (such as figure 1 ) includes: Selective Light Sheet Illumination Microscopic Imaging first collects volume data at a certain angle, then rotates the sample and collects again, and obtains volume data at multiple angles in turn ( figure 1 -S1); For imaging at different angles, calculate the scale-invariant features of the data, perform registration, find the center of rotation and cluster optimization ( figure 1 -S2); For imaging at different angles, perform rotation transformation, and use the nearest neighbor principle and weighted average to fuse volume data from multiple angles ( figure 1 -S3).

[0035] The collection of volume data at a certain angle refers to moving the object tube 2-6 without rotating the object tube 2-6 to obtain a collection of multiple images. The said rotating and re-collecting the sample refers to rotating the loading tube 2-6. image 3 shows the collected results;

[0036] During the sample rotation described ab...

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Abstract

The invention discloses a multi-angle based selective light-sheet illumination microscopy imaging reconstruction method, and relates to the technical field of light-sheet illumination microscopy imaging. In order to eliminate influences imposed on the image quality by strip artifacts, scattering fuzz, uneven gray and transverse and axial resolution difference in a selective plane illumination microscopy (SPIM) imaging technology, the method proposes that scale-invariant features are extracted in allusion to microscopic body data acquired at different angles, matching is performed, a rotation center is solved through a clustering algorithm, and then reconstruction is performed by using a nearest neighbor algorithm and weighted averaging so as to clear body data of a sample. According to the invention, multi-angle images acquired by a selective light-sheet illumination microscopy imaging system are fused, image noises and scattering fuzz are effectively suppressed, and the longitudinal resolution of microscopic images is improved.

Description

technical field [0001] The invention relates to the technical field of light sheet illumination microscopic imaging (SPIM), in particular to a reconstruction method based on multi-angle selective light sheet illumination microscopic imaging. Background technique [0002] Selective Light Sheet Illumination Micro Imaging System (SPIM): A layer of light beam is used to excite the fluorescent sample, and the imaging is detected by the image sensor (CCD), while the incident illumination light path and the CCD receiving fluorescence light path are perpendicular to each other, and the incident light surface is adjusted by moving the sample. Different planes are excited to obtain a 3D image of the whole tissue while maintaining the resolution at the cellular level. SPIM is an excellent analysis tool for tissue structure and morphology. It can achieve the maximum imaging depth of large biological samples without slicing the sample. It can quickly and conveniently study phenotype, mou...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/521G06T7/55G06T17/00
CPCG06T17/00G06T2200/04G06T2207/10056
Inventor 田捷胡朝恩惠辉董迪杨鑫
Owner INST OF AUTOMATION CHINESE ACAD OF SCI
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