Coherent anti-stokes Raman scattering spectrum detection and microimaging system and method

Abstract

The invention relates to the field of optics, and provides a coherent anti-stokes Raman scattering spectrum detection and microimaging system which comprises a femtosecond pulse laser device, a beam divider, a pohotonic crystal fiber, a narrow bandpass filter, a delay unit, a beam combiner and a detector, wherein the beam divider is used for dividing femtosecond pulse laser into a first light beam and a second light beam; the pohotonic crystal fiber is used for pumping the first light beam to output super-continuum spectrum laser of which the pulse width is picosecond grade as Stokes light; the narrow bandpass filter is used for filtering the second light beam to increase the pulse width to be approximate to that of the super-continuum spectrum laser as pumping light and detection light; the delay unit is used for synchronizing the stokes light, the pumping light and the detection light; the beam combiner is used for combining the Stokes light, the pumping light and the detection light into a third light beam; the detector is used for detecting a correlated anti-Stokes Raman scattered spectrum. By adopting the coherent anti-stokes Raman scattering spectrum detection and microimaging system, simultaneous imaging of multiple chemical bands can be rapidly achieved, a super-continuum spectrum light source is lowly required, a complete CARS (Coherent Anti-Stokes Raman Scattering) spectrum can be obtained, the light beam utilization efficiency can be maximized, and the spectral resolution of CARS imaging can be improved.

Description

technical field [0001] The invention belongs to the technical field of spectral detection and microscopic imaging, in particular to a coherent anti-Stokes Raman scattering spectral detection and microscopic imaging system and method. Background technique [0002] Coherent anti-Stokes Raman scattering (CARS) microscopy imaging technology can obtain anti-Stokes signals according to the vibration or rotation characteristics of material molecules without the need for external labels. Good chemical specificity, high sensitivity and high three-dimensional chromatography ability, so it is widely used in the field of biology for lipid molecular imaging, selective imaging of proteins and nucleic acids, etc. and convenient label-free imaging. For a long time, CARS microscopy imaging technology has mostly been based on the CARS signal of a specific chemical bond in the molecule to characterize a specific molecule. Obviously, it is far from enough to rely on a single chemical bond to ...

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a coherent anti-Stokes Raman scattering spectrum detection system, aiming to solve the problem that traditional CARS technology cannot obtain a complete CARS spectrum with high spectral resolution while reducing the requirements for supercontinuum light sources

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