The invention discloses a blue-
green algae type discrimination method based on a three-dimensional
fluorescence spectrum. The method comprises the following steps: S0, obtaining a to-be-detected sample; the method comprises the following steps: S1, acquiring original three-dimensional
fluorescence spectrum data; s2, preprocessing the original three-dimensional
fluorescence spectrum data;
rayleigh scattering and
Raman scattering in the original three-dimensional fluorescence spectrum are removed, and smooth denoising is carried out on the original three-dimensional fluorescence spectrum; s3, spectral features are extracted based on a parallel factor-peak
ratio method, and spectral feature vectors are obtained; and S4, carrying out missing value supplement and data
standardization processing on the obtained spectral feature vectors, inputting the processed feature vectors into a trained LightGBM multi-classifier, outputting probabilities by a classification model, and selecting a category with the maximum probability. According to the method, the distribution mechanism of the blue-
green algae fluorescent
pigment is combined, and the fluorescent component characteristics and the relative content characteristics of the fluorescent components of the blue-
green algae are comprehensively considered, so that the three-dimensional fluorescence spectrum information of the blue-green
algae sample can be more comprehensively, completely and meticulously represented, and the variety discrimination of different blue-green
algae with smaller difference is realized.