Method for quickly screening and identifying various paralytic shellfish toxins in red tide algae
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A technology of red tide algae and shellfish toxin, which is applied in the field of identification of red tide algae, can solve the problem of not being able to screen and identify paralytic shellfish toxin, and achieve the effect of rapid identification
Inactive Publication Date: 2017-08-29
连云港市海洋环境监测预报中心
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Its disadvantage is that it can only screen specific paralytic shellfish toxins against the characteristic mass spe
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Embodiment 1
[0030] Embodiment 1, a method for rapid screening and identification of multiple paralytic shellfish toxins in red tide algae, the steps are as follows:
[0031] (1) Weigh an appropriate amount of red tide algal algae samples, put them in a centrifuge tube, add an appropriate amount of extraction solvent, use a cell crusher or algae grinding method to crush, then extract some paralytic shellfish toxins, and combine the extracts; The liquid was centrifuged to remove the precipitate, and the supernatant was filtered through a microporous membrane, poured into a sample bottle, and set aside;
[0032] (2) High-performance liquid chromatography-high-resolution mass spectrometry was used to separate and analyze the paralytic shellfish toxins in the crude extract of red tide algae.
[0033] (3) By comparing with the information related to the precise molecular mass of common paralytic shellfish toxins in red tide algae: the relevant information includes the molecular formula of the t...
Embodiment 2
[0036] Example 2, a method experiment for rapid screening and identification of various paralytic shellfish toxins in red tide algae:
[0037] 1.1 Instruments and reagents
[0038] High-performance liquid chromatography, high-resolution mass spectrometer, equipped with electrospray ion source, high-power numerically controlled ultrasonic instrument, ultrasonic cell pulverizer, high-speed refrigerated centrifuge, and ultrapure water treatment system.
[0039]Chromatographically pure formic acid, chromatographically pure acetonitrile, ammonium formate is superior grade, and ultrapure water is produced by Mili-Q ultrapure water system. A variety of common paralytic shellfish toxin standard solutions include CRM-STX-e, CRM-NEO-b, CRM-GTX1&4-c, CRM-GTX2&3-c, CRM-dcGTX2&3-c, CRM-C1&2, etc.
[0040] 1.2. Preparation of reference solution
[0041] Dilute the standard solution of bismuth shellfish toxin with 0.1 mol / L HAC so that the concentration of each component is STX (0.97 mg / L)...
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Abstract
The invention relates to a method for quickly screening and identifying various paralytic shellfish toxins in red tide algae. The method comprises the following steps: weighing a certain quantity of red tide algae samples, putting into a centrifugal tube, adding a certain quantity of extracting solvents, crushing by adopting a cell crusher or an algae grinding method, then extracting the various paralytic shellfish toxins and combining into an extracting solution; performing centrifugal separation to remove a precipitate, absorbing supernatant liquid, filtering through a micro-porous filtering membrane and injecting into a sample bottle for later use; performing separation analysis on the paralytic shellfish toxins in the red tide algae crude extracting liquid by adopting a high-performance liquid chromatography-high resolution mass spectrometry; through comparing information related to the accurate molecular weight of the common paralytic shellfish toxins in the red tide algae, the quick identification on the various common paralytic shellfish toxins in the red tide algae is realized. The method provided by the invention can be used in a department of marine environmental management to quickly determine whether the paralytic shellfish toxins are contained in the red tide algae or not and is further used for judging the influence of the marine shellfish toxins on aquatic products to ensure the consumption safety of the aquatic products.
Description
technical field [0001] The invention relates to a method for identifying red tide algae, in particular to a method for quickly screening and identifying various paralytic shellfish toxins in marine red tide algae. Background technique [0002] In recent years, with the increasing pollution of the marine environment, the frequency of red tides is increasing day by day, and human poisoning and death incidents caused by red tide toxins are not uncommon. Paralytic shellfish toxins (PSP) are the most toxic, most widely distributed, most poisonous and most serious toxins among red tide toxins. Paralytic shellfish toxin is a kind of neurotoxin, which can hinder the conduction of nerve impulses and inhibit the central and peripheral nerves. Ingesting a small amount of paralytic shellfish toxin can cause nervous system diseases, such as tremors and excitement. In severe cases, it can cause Respiratory paralysis and even death. Therefore, establishing a rapid and accurate method for...
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