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Method for detecting paralytic shellfish poisoning

A shellfish toxin and paralytic technology, applied in the direction of material impedance, can solve the problems of high requirements for operators, failure to discover new toxins, high detection limit, etc., and achieve the effect of small interference, reliable data and low cost

Inactive Publication Date: 2014-12-10
ZHEJIANG UNIV
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Problems solved by technology

For example, although the mouse biological method is simple to operate and does not require complicated techniques and equipment, the detection limit is relatively high, and it is opposed by animal protectionists; the liquid chromatography fluorescence method not only has problems such as expensive equipment and high requirements for operators. , there is still the disadvantage of not being able to discover new toxins

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  • Method for detecting paralytic shellfish poisoning
  • Method for detecting paralytic shellfish poisoning
  • Method for detecting paralytic shellfish poisoning

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Embodiment Construction

[0020] Such as figure 2 As shown, the change of cell impedance value is related to cell number and morphology. The cell impedance sensor is a technology that detects changes in cell number, attachment, and growth status through changes in cell impedance values. It has the advantages of real-time detection, label-free, and non-damage detection. When there is no cell growth on the chip, the impedance value Z=Z 0 , as the cells attach and grow, the impedance value Z=Z cell . The cell index (CI) is a parameter often used to characterize the cell state, so the cell index can be calculated according to the formula CI=(Z cell -Z 0 ) / Z 0 Calculated. Veratridine is an activator of sodium channels, which can promote the influx of sodium ions, while ouabain is an inhibitor of sodium pumps. The direct consequence of the combined action of these two drugs on neuro2a cells is to cause a large influx of extracellular sodium ions , causing cells to swell and die. The paralytic shellf...

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Abstract

The invention discloses a method for detecting paralytic shellfish poisoning. The method is implemented on a mouse neuroma cell impedance sensor. The method comprises the following steps: inoculating neuro2a cells to sensor chip cell culture holes for culturing; respectively adding a to-be-detected solution into cell culture holes a solution containing ouabain and veratridine; and detecting a CI value of each cell culture hole after the to-be-detected solution is added, and judging the content of the paralytic shellfish poisoning in the to-be-detected solution according to change of the CI value. According to the method, cells are directly taken as detection carriers, the operation is simple, and the cost is low. According to the method, the detection limit of saxitoxin is low to 0.9ng / ml and has obvious progress compared with the detection limit (160ng / ml) of mouse bioassay in a current national standard detection method. In addition, the method is slightly influenced by interference effect of shellfish meat matrixes and reliable in data.

Description

technical field [0001] The invention relates to a method for detecting paralytic shellfish toxin. Background technique [0002] Paralytic shellfish toxin is a small molecular compound of alkyl hydrogenated purines produced by marine algae, which can be enriched in shellfish through the filter-feeding effect of shellfish. Once people accidentally eat shellfish contaminated by this toxin, the human body will produce neurotoxic reactions, such as: slight tingling, numbness of limbs and lips, headache and fever, etc., which may be life-threatening in severe cases. These symptoms usually occur between 10 minutes and 15 hours after ingestion. In addition, the paralytic toxin is also one of the most widely distributed and harmful marine toxins in the world, and there is no specific antidote so far. Therefore, the detection of paralytic shellfish toxin is particularly important for the protection of human health and food safety. [0003] As the official detection method recommend...

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Application Information

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IPC IPC(8): G01N27/02
Inventor 王平邹玲黎洪波周洁胡宁苏凯麒杜立萍王琴
Owner ZHEJIANG UNIV
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