42 results about "Liquid Chromatography-Fluorescence" patented technology

A method for determining monoamine neurotransmitters and metabolites thereof in human urine through solid-phase extraction-high performance liquid chromatography-fluorescence spectrometry includes thefollowing steps: S1, preprocessing a sample; S2, preparing a sample: performing solid phase extraction by using a self-made solid phase extraction column; S3, preparing a standard solution; S4, drawing a standard curve; and S5, determining: introducing the sample obtained in step S2, performing HPLC analysis, qualitatively analyzing the retention time, and quantifying the peak area. Cellulose acetate is used as a carrier, pomelo peel and flesh powder is used as an adsorbent, the pomelo peel and flesh powder is evenly distributed in the gaps of the cellulose acetate, and the peel and flesh powder has excellent sensitivity and selectivity to the monoamine neurotransmitters and the metabolites thereof in the urine, so the monoamine neurotransmitters and the metabolites thereof in the human urine are enriched in pomelo peel and flesh powder, the purity and the sensitivity of the sample are greatly improve, the detection limit is reduced, the recovery rate is improved, and the detection efficiency is improved; and the cost of the method is low, so the detection cost is reduced.

The invention discloses a method for measuring residual quantity of diethylstilbestrol in aquatic products by utilizing a liquid chromatogram fluorescent method, comprising the following steps: (1) weighing 100g of ground aquatic product samples in a 50mL plastic centrifuge tube, adding 20mL of acetic ether, homogenizing for 230s, centrifuging for 10min with the speed of 7000r / min, moving the acetic ether layer to a 100mL pear-shaped flask, utilizing 2mL of normal hexane and acetic ether with a volume ratio of 85:15 to dissolve residue, and then stirring for 6min; (2) activating an SLH column by utilizing 5mL of normal hexane and 5mL of acetic ether, adding extracting solution on the SLH column, utilizing 10mL of normal hexane and acetic ether with the volume ratio of 82:20 to elute by controlling the flow rate at 2mL / min, collecting eluent, carrying water bath at the temperature of 450 DEG C, and then carrying out nitrogen drying till the original state; and (3) adding 1mL of sulfuric acid to the centrifuge tube of nitrogen drying, then adding 8mL of water in the centrifuge tube, utilizing 1mL of mobile phase for metering volume so as to be measured by an efficient liquid phase chromatogram. The method for measuring residual quantity of diethylstilbestrol in aquatic products has advantages of low cost, simple method, and low measuring limit, is suitable for popularization and use in the production and practice and is a technology demanded by the market, and has positive social meaning and social demand.

The invention provides a method for measuring the content of seven harmful phenol in the mainstream flue gas by high efficiency liquid chromatography, comprising the steps of mainstream flue gas collection, flue gas sample preparation and high efficiency liquid chromatography fluorescence detection. Firstly, the fluorescence detection spectrum data output by a high efficiency liquid chromatographyfluorescence detector of different mixed concentration sample is processed with a partial least square method, and a mathematical model is built, and then the fluorescence detection spectrum data ofthe metacresol and the paracresol in high efficiency liquid chromatography fluorescence detection data of the flue gas sample is input into the mathematical model to obtain the concentration of the metacresol and the paracresol. The method can be used for simultaneously analyzing the content of seven harmful phenol compounds in the mainstream flue gas and realizes the measurement of the metacresolcompound and the paracresol compound with a chemometrics method without adopting any other instrument, equipment or reagent, has accurate mausruing result, simple measure process and rapid calculation speed.

The invention relates to a detection method applicable to common phosphonate esters in water bodies with different salinities, and relates to a detection method for phosphonate esters in water bodies. A detection method suitable for common phosphonate esters in different salinity water bodies is provided, which has the advantages of simple operation, low requirements on instruments and the ability to simultaneously determine four phosphonate esters. A method for separating and measuring the concentration of common phosphonates in water—glyphosate, glufosinate, and aminomethylphosphonic acid 2-aminoethylphosphonic acid by using high-performance liquid chromatography fluorescence method. The specific steps are as follows: using fluorenyl methaneoxycarbonyl chloride as a derivatizing agent to react with phosphonate, and the reaction product enters high performance liquid chromatography for separation and determination after being filtered. It has the advantages of simple operation, low requirements for instruments, short sample determination time, and simultaneous determination of four phosphonate esters, etc., and can be applied to the determination of phosphonate esters in water bodies with different salinities.

The invention relates to an amino functional group chiral compound resolution mark band fluorescence derivatizing agent, in particular to a DBD-trans-2-methyl-L-proline (DBD-M-Pro) agent and a DBD-(2-succinimidoxy)-trans-2-methyl-L-proline (DBD-M-Pro) agent. The two agents contain benzofurazan as a fluorescence functional group and a chiral center structure, can carry out fluorescence marking on an amino functional group chiral compound and establishes an analysis method with high sensitivity and high selectivity in amino functional group chiral compound resolution by utilizing a liquid chromatography-fluorescence detection (LC-FL) technology. Since containing the fluorescence functional group self, the agent can remarkably improve the detection sensitivity in a fluorescence detector, can also carry out chiral resolution on the amino functional group chiral compound in reversed phase chromatography and supplies a novel high-sensitivity fluorescence chiral derivatizing agent for studying resolution of an amino functional group chiral metabolite and screening various disease chiral biomarkers.

The invention discloses a method for detecting ochratoxin A in capsicum extract. The method comprises the following steps: (1) performing ultrasonic extraction on a capsicum extract sample by using an alcoholic solution, so as to obtain an extracting solution; (2) freezing and centrifugally separating the extracting solution, so as to obtain the supernatant; (3) diluting the supernatant by using a PBS solution, and filtering to obtain the filtered liquid; (4) concentrating the filtered liquid to be dry, and adding a solvent for ultrasonic dissolving, so as to obtain a dissolving solution; (5) detecting the content of the ochratoxin A in the dissolving solution by adopting a liquid chromatography-fluorescence method. According to the method, toxins can be fully extracted from the sample, the impurities are removed by freezing and centrifuging, the sample is further purified by a PBS solution, an immunoaffinity column is not used, batch sample treatment is facilitated, and the effects of improving the detection efficiency, reducing the cost, saving energy and reducing consumption can be achieved. According to the method, the extracting solution is diluted and filtered by PBS and directly concentrated and detected, the detection limit is 1 [mu]g / kg, and the detection requirement is met.