Detection method of quinolone medicine residues

A quinolone and detection method technology, which is applied in the detection field of quinolone drug residues, can solve the problems of high consumption of organic solvents, cannot be reused, complicated operations, etc., and achieves low cost, less use of organic solvents, and good repeatability. Effect

Inactive Publication Date: 2017-11-17
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commonly used sample pretreatment methods include liquid-liquid extraction, supercritical fluid extraction, QuEChERS, and solid-phase extraction. Traditional pretreatment methods are time-consuming, complicated to operate, consume a lot of organic solvents, and are expensive and cannot be reused. Extraction is widely used in food testing, environmental monitoring and forensic diagnosis because of its advantages of simple operation and small amount of sample and solvent.

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  • Detection method of quinolone medicine residues
  • Detection method of quinolone medicine residues
  • Detection method of quinolone medicine residues

Examples

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Effect test

Embodiment 1

[0024]Put the glass beads into a mixed solution (7:3, V / V) of concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30%, heat in an 80°C water bath for 1h, and rinse with ultrapure water and pure ethanol in turn Afterwards, it was placed in ATPES ethanol solution (components by volume, including 5% APTES, 5% deionized water and 90% pure ethanol) to react for 24h, washed with ultrapure water and pure ethanol successively, placed in 80 Under high-purity nitrogen flow overnight, after 15 hours, take out the glass microspheres and activate them with 2.5% glutaraldehyde PBS solution for 5 hours. Shake for 24 hours under the condition of 4 on the shaker, wash with ultrapure water, place in a 0.3M ethanolamine solution with a pH of 7.5 to inactivate unreacted glutaraldehyde, take out the glass beads after 1 hour and put them in 0.2mg / mL of NaCNBH 3 In the solution for 24h, the imide is converted into an amine and the covalent bond is s...

Embodiment 2

[0030] Put the glass beads into a mixed solution (7:3, V / V) of concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30%, heat in an 80°C water bath for 1h, and rinse with ultrapure water and pure ethanol in turn Afterwards, it was placed in ATPES ethanol solution (components by volume, including 5% APTES, 5% deionized water and 90% pure ethanol) to react for 24h, washed with ultrapure water and pure ethanol successively, placed in 80 Under high-purity nitrogen flow overnight, after 15 hours, take out the glass microspheres and activate them with 2.5% glutaraldehyde PBS solution for 5 hours. Shake for 24 hours under the condition of 4 on the shaker, wash with ultrapure water, place in a 0.3M ethanolamine solution with a pH of 7.5 to inactivate unreacted glutaraldehyde, take out the glass beads after 1 hour and put them in 0.2mg / mL of NaCNBH 3 In the solution for 24h, the imide is converted into an amine and the covalent bond is ...

Embodiment 3

[0036] Put the glass beads into a mixed solution (7:3, V / V) of concentrated sulfuric acid with a mass fraction of 98% and hydrogen peroxide with a mass fraction of 30%, heat in an 80°C water bath for 1h, and rinse with ultrapure water and pure ethanol in turn Afterwards, it was placed in ATPES ethanol solution (components by volume, including 5% APTES, 5% deionized water and 90% pure ethanol) to react for 24h, washed with ultrapure water and pure ethanol successively, placed in 80 Under high-purity nitrogen flow overnight, after 15 hours, take out the glass microspheres and activate them with 2.5% glutaraldehyde PBS solution for 5 hours. Shake for 24 hours under the condition of 4 on the shaker, wash with ultrapure water, place in a 0.3M ethanolamine solution with a pH of 7.5 to inactivate unreacted glutaraldehyde, take out the glass beads after 1 hour and put them in 0.2mg / mL of NaCNBH 3 In the solution for 24h, the imide is converted into an amine and the covalent bond is ...

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Abstract

The invention relates to a detection method of quinolone medicine residues. The method comprises the following steps: 1, filling an injector with a glass bead coupled with a quinolone monoclonal antibody to obtain an immunoaffinity syringe; 2, extracting a sample to be detected by using the immunoaffinity syringe, and carrying out elution to obtain an eluate; and 3, blowing the eluate with nitrogen until the eluate is dry, re-dissolving the dried eluate in a mobile phase, and carrying out high performance liquid chromatographic analysis. The method for detecting quinolone medicines residual in milk through an immunoaffinity syringe microextraction-liquid chromatography-fluorescence technology, established through combining an immunoaffinity adsorbent with MEPS, has the advantages of good selectivity, high sensitivity, simplicity in operation, good repeatability, low cost, small use amount of an organic solvent, and environmental protection.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection method for quinolone drug residues. Background technique [0002] Quinolones are a class of broad-spectrum antibiotics containing the basic structure of pyruvate. They have inhibitory effects on Gram-positive bacteria, mycoplasma and Gram-negative bacteria. The main mechanism of action is to inhibit the catalysis of bacterial DNA helicase. In order to improve production, quinolones (Quinolones, QNs) are widely used in aquaculture, but due to the existence of cytotoxicity, bacterial resistance and multi-drug resistance, the residual problem of QNs has attracted more and more attention. Both the European Union and China have stipulated that danofloxacin, difloxacin, and enrofloxacin (the sum of ciprofloxacin and enrofloxacin) in muscle, fat, and liver of cattle, chicken, pigs, and sheep, The maximum residue limit of QNs such as sarafloxacin is 0.01-1.9mg / kg. . ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/06G01N33/577G01N33/552
CPCG01N30/02G01N30/06G01N30/74G01N33/552G01N33/577G01N2030/062
Inventor 李存张欣达赵世瑾杨琳燕张伟王翠翠郑妍
Owner TIANJIN AGRICULTURE COLLEGE
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