Method for detecting aflatoxin B1 in vegetable oil based on concentrated sulfuric acid low-temperature sulfonation purification

By combining low-temperature controlled hexane dissolution with concentrated sulfuric acid sulfonation, the problems of matrix interference and target analyte destruction in the detection of aflatoxin B1 in vegetable oils have been solved, realizing an efficient and low-cost AFB1 detection method applicable to a variety of vegetable oil matrices.

CN121410131APending Publication Date: 2026-01-27ZHEJIANG FORESTRY ACAD +1
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Patent Information

Application Number
CN202511338716.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-18
Publication Date
2026-01-27

AI Technical Summary

Technical Problem

Existing methods for detecting aflatoxin B1 in vegetable oils are severely affected by matrix interference, and traditional sulfonation methods easily destroy the target analyte at room temperature, resulting in inaccurate detection results and high costs.

Method used

By combining low-temperature controlled hexane dissolution with concentrated sulfuric acid sulfonation, and carrying out the sulfonation reaction in an ice-water bath, combined with detection by liquid chromatography-fluorescence detector, efficient sample purification and protection of target analytes are achieved.

Benefits of technology

It achieves high sensitivity and high accuracy in AFB1 detection, with a detection limit lower than the national standard, reducing detection costs and the amount of organic solvents used, and is suitable for a variety of vegetable oil matrices.

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Abstract

The invention relates to the technical field of food safety detection, and discloses a method for detecting aflatoxin B1 in vegetable oil based on concentrated sulfuric acid low-temperature sulfonation purification, which comprises the following steps: dissolving a vegetable oil sample by using n-hexane, slowly adding concentrated sulfuric acid under the strict control of ice-water bath (0-4 DEG C) to carry out sulfonation purification reaction, the aflatoxin B1 is protected from being degraded while the impurities are effectively removed; and after derivatization, detecting by using a liquid chromatography-fluorescence detector. The innovation of the invention lies in that a strict low-temperature reaction system is introduced, and the technical problem that a concentrated sulfuric acid sulfonation method may damage a target analyte is solved. According to the method, the detection limit can reach 0.05 mu g / kg, the quantification limit can reach 0.15 mu g / kg, the recovery rate is high (85-105%), the relative standard deviation is smaller than 5%, the cost is low, and the method is remarkably superior to an existing detection method.
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