Analysis method for efficiently detecting piperazine residues in chicken tissue, eggs and pork

An analysis method and tissue technology, applied in the field of high-efficiency detection of piperazine residues in chicken tissues, eggs and pork

Inactive Publication Date: 2018-07-17
YANGZHOU UNIV
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Problems solved by technology

[0002] At present, there are gas chromatography, high performance liquid chromatography, gas chromatography-tandem mass spectrometry, high performance liquid chromatography-tandem mass spectrometry for the detection of piperazine at home and abroad, but pre-column derivatization-ultra high performance liquid chromatography fluorescence detection (UPLC- FLD) detection method for piperazine residues in animal-derived tissues and foods has not been reported yet.

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  • Analysis method for efficiently detecting piperazine residues in chicken tissue, eggs and pork
  • Analysis method for efficiently detecting piperazine residues in chicken tissue, eggs and pork
  • Analysis method for efficiently detecting piperazine residues in chicken tissue, eggs and pork

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[0030] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0031] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. These examples are only for illustration of the present invention and do not limit the scope of the present invention in any way.

[0032] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0033] 1. Breeding and Sample Collection of Experimental Poultry

[0034] In this experiment, 12 112-day-old Jinghai Yellow Chickens (Jiangsu Jinghai Poultry ...

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Abstract

The invention relates to the field of veterinary drug residue detection, in particular to an analysis method for efficiently detecting piperazine residues in chicken tissue, eggs and pork. The methodcomprises the steps of extracting and purifying the chicken tissue, eggs or pork samples through an accelerated solvent extraction method and a solid phase extraction method, performing derivatizationwith dansyl chloride and then performing detection by using an ultra-high performance liquid chromatography fluorescence detection method. Compared with other detection methods, the method has the advantages of higher analysis efficiency, less mobile phase consumption, a high recovery rate, high accuracy, high sensitivity and good reproducibility.

Description

technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to an analytical method for efficiently detecting piperazine residues in chicken tissues, eggs and pork. Background technique [0002] At present, there are gas chromatography, high performance liquid chromatography, gas chromatography-tandem mass spectrometry, high performance liquid chromatography-tandem mass spectrometry for the detection of piperazine at home and abroad, but pre-column derivatization-ultra high performance liquid chromatography fluorescence detection (UPLC- FLD) detection method for piperazine residues in animal-derived tissues and foods has not been reported yet. Therefore, the present invention establishes for the first time a pre-column derivatization-UHPLC fluorescence detection method for piperazine residues in chicken tissues, eggs and pork. The method has simple elution procedures, less solvent consumption, higher analysis efficiency,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 谢恺舟张杨杨庞茂达谢星王雅娟赵霞王波卜晓娜郭亚文刘楚君张跟喜戴国俊王冉高玉时张涛王金玉
Owner YANGZHOU UNIV
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