Quality control method of traditional Chinese medicine composition for treating chronic renal failure

Abstract

The invention relates to the technical field of a traditional Chinese medicine preparation, and particularly relates to a quality control method of a traditional Chinese medicine composition for treating chronic renal failure. The quality control method comprises the following steps: establishing a fingerprint spectrum of the traditional Chinese medicine composition by adopting a high performance liquid chromatography with the chromatographic condition of taking octadecyl silane as a filling agent, taking a 0.2% phosphoric acid solution as a mobile phase A and taking acetonitrile as a mobile phase B; preparing a test solution, namely, putting 0.1-1g of the traditional Chinese medicine composition for treating chronic renal failure into a 50ml of a centrifugal pipe, adding 5-20ml of ultrapure water, carrying out ultrasound treatment for 10-30 minutes, cooling, mixing evenly, then centrifuging, taking liquid supernatant, filtering by a 0.45micron of microporous filtering membrane, and taking subsequent filtrate to obtain a test solution; and determining, namely, sucking the test solution precisely, pouring into a liquid chromatograph, and recording a chromatogram. The quality control method is rapid, simple, and convenient; by adopting once extraction and simultaneous determining, the operation steps are reduced, the time and cost are saved, and thus the analysis efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a quality control method of a traditional Chinese medicine composition for treating chronic renal failure. Background technique [0002] At present, traditional Chinese medicine has unique features in disease prevention and treatment. Chronic renal failure belongs to the categories of edema, dysentery, and off-grade in Chinese medicine. The pathogenesis is based on deficiency in origin and excess in superficiality. Although dialysis or kidney transplantation is the treatment for the end-stage survival of patients with chronic renal failure, in the early and middle stages of chronic renal failure, on the basis of treating the primary disease, effective drug treatment measures can be used to effectively prevent or delay the disease. Progression of chronic renal failure is an ideal method. [0003] Studies have shown that traditional Chinese medic...

AI Technical Summary

Problems solved by technology

But above-mentioned compound Chinese medicine curative effect is single, and activity is weak

[0004] Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation of their quality should adopt appropriate quality control methods that can provide rich identification information, but the current methods of microscopic identification, physical and chemical identification and content determination are not enough to solve this problem

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