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Quantitative detection method for tilmicosin residues in poultry meat, pork or poultry eggs

A quantitative detection method, the technology of tilmicosin, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of tilmicosin residues, etc., and achieve high precision and sensitivity, accurate qualitative and quantitative, and good sensitivity Effect

Pending Publication Date: 2021-12-03
YANGZHOU UNIV
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Problems solved by technology

[0004] In order to solve the problem of extracting and purifying tilmicosin residues and derivatization from poultry meat, pork and poultry eggs

Method used

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  • Quantitative detection method for tilmicosin residues in poultry meat, pork or poultry eggs
  • Quantitative detection method for tilmicosin residues in poultry meat, pork or poultry eggs
  • Quantitative detection method for tilmicosin residues in poultry meat, pork or poultry eggs

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Embodiment Construction

[0046] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0047] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. These examples are only for illustration of the present invention and do not limit the scope of the present invention in any way.

[0048] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0049] (1) Breeding and sample collection of experimental livestock and poultry

[0050] The conduct of this experiment has been approved by the Ethics Commit...

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Abstract

The invention relates to the field of veterinary drug residue detection, in particular to a quantitative detection method for tilmicosin residues in poultry meat, pork or poultry eggs. The method comprises the following steps of sequentially extracting the poultry meat, the pork or the poultry eggs by acetonitrile, degreasing by normal hexane, purifying an extracting solution by an HLB solid-phase extraction column, and blow-drying and concentrating an eluent by nitrogen; adding the pyridine and acetic anhydride into the concentrated and dried sample, filtering after derivative reaction, and detecting filtrate by GC-MS / MS detection. According to the present invention, the derivative product obtained by adopting the extraction method combining the liquid-liquid extraction, solid-phase extraction and gel chromatography is good in peak pattern and few in interference impurity peak, and the response value of the sample derivative product is high.

Description

technical field [0001] The invention relates to the field of veterinary drug residue detection, in particular to a pre-column derivatization-gas chromatography-tandem mass spectrometry confirmation analysis method for detecting tilmicosin residues in poultry meat, pork or poultry eggs. Background technique [0002] At present, the pretreatment methods for tilmicosin residues in animal foods at home and abroad mainly include liquid-liquid extraction and solid-phase extraction, and the extraction reagents include organic solvents such as acetonitrile, alkyl halides, esters, and ethers. Due to the high molecular weight and strong polarity of tilmicosin, direct detection by gas chromatography or gas chromatography-mass spectrometry is not feasible. Therefore, the present invention uses hydrobromic acid for hydrolysis to reduce the molecular weight of the drug, and then uses acetic anhydride as a derivatization reagent to derivatize tilmicosin. So far, the extraction of tilmicos...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72G01N30/86G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N30/8634G01N30/88G01N2030/062G01N2030/065G01N2030/067G01N2030/884
Inventor 谢恺舟贺兆源姜军华王旭堂谢星庞茂达王波卢阳郭亚文陈兰陈晋元张跟喜张涛戴国俊王冉杨成根包文斌高玉时
Owner YANGZHOU UNIV
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