Method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph
A technology of high performance liquid chromatography and hydroxyacetoquinone, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of prolonged blood coagulation time, and achieve high accuracy, simple method, and good reproducibility
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[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0024] In this embodiment, the instrument selected for the method of determining acequinone and hydroxyacequinone by using a high performance liquid chromatography is: a 1200 type high performance liquid chromatography with a diode array detector from Agilent Corporation of the United States; Model 2450 UV-Vis spectrophotometer of Tianjin Company; BILONJJ-2 high-speed tissue grinder of Jiangsu Jintan Yitong Electronics Co., Ltd.; R-210 rotary evaporator of Swiss Buchi Co., Ltd.; 21011V001 of Swiss Buchi Co., Ltd. R200 nitrogen blowing instrument; TB215B electronic balance from Denver, USA; Synery UV ultrapure water system from Millipore, USA.
[0025] The reagents used were: HPLC grade n-hexane from MERCK MILLIPORE, Germany, HPLC grade ethyl acetate from MERCK MILLIPORE, Germany, and HPLC grade acetonitrile from Mallinckroat Baker; ex...
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