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Method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph

A technology of high performance liquid chromatography and hydroxyacetoquinone, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of prolonged blood coagulation time, and achieve high accuracy, simple method, and good reproducibility

Inactive Publication Date: 2014-07-02
曾铭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acequinone is non-irritating to the skin and eyes, and will not cause skin allergies, but it is considered an antagonist of vitamin K, which will affect the blood clotting system, prolong the blood clotting time, and cause internal bleeding

Method used

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  • Method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph
  • Method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph
  • Method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] In this embodiment, the instrument selected for the method of determining acequinone and hydroxyacequinone by using a high performance liquid chromatography is: a 1200 type high performance liquid chromatography with a diode array detector from Agilent Corporation of the United States; Model 2450 UV-Vis spectrophotometer of Tianjin Company; BILONJJ-2 high-speed tissue grinder of Jiangsu Jintan Yitong Electronics Co., Ltd.; R-210 rotary evaporator of Swiss Buchi Co., Ltd.; 21011V001 of Swiss Buchi Co., Ltd. R200 nitrogen blowing instrument; TB215B electronic balance from Denver, USA; Synery UV ultrapure water system from Millipore, USA.

[0025] The reagents used were: HPLC grade n-hexane from MERCK MILLIPORE, Germany, HPLC grade ethyl acetate from MERCK MILLIPORE, Germany, and HPLC grade acetonitrile from Mallinckroat Baker; ex...

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Abstract

The invention discloses a method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph. The method comprises the following steps: chopping an edible part of a sample, smashing, uniformly mixing, weighing the sample, putting the sample into a centrifuge tube, adding n-hexane and ethyl acetate, homogenating to extract, adding anhydrous sodium sulfate, homogenating to extract again, performing centrifugal treatment, putting the supernate into a pear-shaped bottle, rotating and concentrating to be slightly dry in water bath, adding n-hexane and the ethyl acetate, and ultrasonically dissolving to obtain the sample; before the sample is added, firstly, extracting by using a pre-leaching extraction column, subsequently transferring the extract solution to a decontaminating column, furthermore washing the pear-shaped bottle by using a mixture liquid of the n-hexane and the ethyl acetate, transferring into an extraction column, and collecting all effluent; blowing to dry the effluent by using nitrogen in water bath; dissolving by using flow phase, metering the volume to be 1-2mL, and filtering by using a 0.45-micron filtering film, wherein the filtrate is taken as a sample liquid to be measured; and measuring by using the high performance liquid chromatograph according to chromatographic conditions. By adopting the method disclosed by the invention, the content of acequinocyl and hydroxyl acequinocyl can be accurately measured.

Description

technical field [0001] The present invention relates to a method for measuring acequinone and hydroxyacequinone. Background technique [0002] The scientific name of Acequinocyl is 2-(acetoxy) 3-dodecyl-1,4-naphthoquinone, the pure product is light yellow powdery solid, molecular formula C 24 h 32 o 4 , CAS No. 57960-19-7, relative molecular weight 384.51, acarquinone is an important synthetic acaricide pesticide, is used to control lice, mites and other invertebrate insecticides, acarquinone The chemical structure of is as follows. [0003] [0004] The metabolite of acequinone is Hydroxyacequinocyl, scientific name 2-hydroxy-3-dodecyl-1,4-naphthoquinone, CAS number 57960-31-3, molecular formula C 22 h 30 o 3 , relative molecular weight 342.47, light yellow powdery solid, the chemical structure is as follows. [0005] [0006] Acequinone as a contact acaricide. It has no systemic activity and is widely used in fruit trees such as pears, peaches, citrus, cucumb...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 曾铭喻零春蒋小良陈梅
Owner 曾铭
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