A method for determining acequinone and hydroxyacequinone using high performance liquid chromatography

Abstract

The invention discloses a method for measuring acequinocyl and hydroxyl acequinocyl by using high performance liquid chromatograph. The method comprises the following steps: chopping an edible part of a sample, smashing, uniformly mixing, weighing the sample, putting the sample into a centrifuge tube, adding n-hexane and ethyl acetate, homogenating to extract, adding anhydrous sodium sulfate, homogenating to extract again, performing centrifugal treatment, putting the supernate into a pear-shaped bottle, rotating and concentrating to be slightly dry in water bath, adding n-hexane and the ethyl acetate, and ultrasonically dissolving to obtain the sample; before the sample is added, firstly, extracting by using a pre-leaching extraction column, subsequently transferring the extract solution to a decontaminating column, furthermore washing the pear-shaped bottle by using a mixture liquid of the n-hexane and the ethyl acetate, transferring into an extraction column, and collecting all effluent; blowing to dry the effluent by using nitrogen in water bath; dissolving by using flow phase, metering the volume to be 1-2mL, and filtering by using a 0.45-micron filtering film, wherein the filtrate is taken as a sample liquid to be measured; and measuring by using the high performance liquid chromatograph according to chromatographic conditions. By adopting the method disclosed by the invention, the content of acequinocyl and hydroxyl acequinocyl can be accurately measured.

Description

technical field [0001] The present invention relates to a method for measuring acequinone and hydroxyacequinone. Background technique [0002] The scientific name of Acequinocyl is 2-(acetoxy) 3-dodecyl-1,4-naphthoquinone, the pure product is light yellow powdery solid, molecular formula C 24 h 32 o 4 , CAS No. 57960-19-7, relative molecular weight 384.51, acarquinone is an important synthetic acaricide pesticide, is used to control lice, mites and other invertebrate insecticides, acarquinone The chemical structure of is as follows. [0003] [0004] The metabolite of acequinone is Hydroxyacequinocyl, scientific name 2-hydroxy-3-dodecyl-1,4-naphthoquinone, CAS number 57960-31-3, molecular formula C 22 h 30 o 3 , relative molecular weight 342.47, light yellow powdery solid, the chemical structure is as follows. [0005] [0006] Acequinone as a contact acaricide. It has no systemic activity and is widely used in fruit trees such as pears, peaches, citrus, cucumb...

AI Technical Summary

Problems solved by technology

Acequinone is non-irritating to the skin and eyes, and will not cause skin allergies, but it is considered an antagonist of vitamin K, which will affect the blood clotting system, prolong the blood clotting time, and cause internal bleeding

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