High performance liquid chromatography detection method for abamectin content in edible vegetable oil
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A high-performance liquid chromatography and edible vegetable oil technology, which is applied in the detection field of pesticide content in edible vegetable oil, can solve the problems of few residue analysis reports, achieve high specificity and sensitivity, reduce maintenance, and save costs
Inactive Publication Date: 2016-12-14
FOSHAN HAIYUE ZHIDA TECH CO LTD
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Problems solved by technology
Abamectin residue detection objects are concentrated in vegetables, fruits, rice, tea and other matrices, and there are few reports on residue analysis in edible vegetable oils
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Embodiment 1
[0026] 1. Instruments and reagents
[0027] Liquid chromatograph Agilent 1100, FLD, American Agilent Company;
[0028] Gas chromatograph Agilent 6890, ECD, American Agilent Company;
[0033] Anhydrous methanol and acetonitrile are chromatographically pure;
[0034] Abamectin standard substance (purity: 93.0%, Dr. Ehrenstorfer GmbH company, Germany).
[0035] 2. Prepare standard working solution
[0036] (1) Accurately weigh 0.0202g of abamectin in a 100mL volumetric flask, dissolve it with acetonitrile and set the volume to 100mL to obtain No. 1 standard stock solution of 187.86 μg / mL;
[0037] (2) Get the mother liquor 5mL of the above-me...
Embodiment 2
[0057] As described in Example 1, the edible vegetable oil sample B is selected to measure the content of abamectin in the sample.
Embodiment 3
[0059] As described in Example 1, the edible vegetable oil sample C was selected to measure the content of abamectin in the sample.
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Abstract
The invention discloses a high performance liquid chromatography detection method for the abamectin content in edible vegetable oil. The method comprises the steps that a sample is subjected to vortex oscillation extraction through methanol, purified with an ODS C18 solid-phase extraction column, subjected to derivatization through N-methylimidazole and trifluoroacetic anhydride and lastly determined through a liquid chromatography-fluorescence method. According to the method, few reagents are adopted, and the cost is reduced; a sample pretreatment method is simple, the purification effect is good, maintenance of instruments in the using process is reduced, and the technical problems that when a liquid chromatography-ultraviolet detector is used, the sensitivity is too low, and matrix interference is serious are solved; a determined result is accurate and good in repeatability, the detection specificity and sensitivity are high, and a reference is provided for detection of the abamectin content in the edible vegetable oil.
Description
Technical field: [0001] The invention belongs to the technical field of detection of pesticide content in edible vegetable oil, and mainly relates to the detection technology of abamectin content in edible vegetable oil. Background technique: [0002] Abamectin is a class of 16-membered macrocyclic lactone compounds with insecticidal, acaricidal and nematicidal activities. It has stomach poisoning and contact killing effects on mites and insects. It has been widely used in poultry and domestic animals for internal and external parasites. Insects and crop pests, such as parasitic red worms, Diptera, Coleoptera, Lepidoptera and harmful mites. After abamectin is used in various crops such as vegetables, melons and fruits, rice, cotton, rapeseed, and Chinese medicinal materials, it has long-term residual effects on harmful mites and insects that feed on plant tissues. Due to its high efficiency, broad-spectrum characteristics, low side effects, and good environmental compatibil...
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