Detection method suitable for common phosphonate in water bodies with different salinity

Abstract

The invention discloses a method for detecting common phosphonate in water bodies with different salinity, and relates to a method for detecting phosphonate in water bodies. The invention provides a method for detecting common phosphonate in water bodies with different salinity, which is simple to operate, has lower requirements on instruments and can be used for simultaneously detecting four phosphonate. The method can be used for separating and determining the concentrations of common phosphonate-glyphosate, glufosinate-ammonium and aminomethyl phosphonic acid 2-aminoethyl phosphonic acid in the water body by using high performance liquid chromatography fluorescence. The method comprises the following specific steps: reacting fluorenylmethoxycarbonyl chloride serving as a deriving agent with phosphonate, filtering a reaction product, and separating and measuring the reaction product in a high performance liquid chromatography. The method has the advantages of simplicity in operation, low requirement on instruments, short sample determination time, capability of simultaneously determining four phosphonate esters and the like, and can be suitable for determining phosphonate esters in water bodies with different salinity.

Description

technical field [0001] The invention relates to a method for detecting phosphonate in water, in particular to a method for detecting common phosphonate in water with different salinities. Background technique [0002] Organophosphorus is one of the important forms of phosphorus in the ocean and plays a very important role in marine biogeochemical cycles. As a photolysis-resistant, high-temperature-resistant chemically stable C-P bond organic phosphorus, phosphonate is still relatively lacking in various aspects of research. In recent years, C-P bond organophosphorus pesticides represented by glyphosate and glufosinate-ammonium have been widely used all over the world. Accumulation poses a certain threat to environmental safety and human health. On the other hand, biogenic phosphonates represented by 2-aminoethylphosphonic acid widely exist in lower organisms. 2-Aminoethylphosphonic acid can be used as a potential phosphorus source for some organisms in aquatic environment...

AI Technical Summary

Problems solved by technology

However, liquid chromatography-mass spectrometry is expensive and the operation is complicated, so it is not suitable for popularization and use.

In addition, most of the existing phosphonate determination methods are only for the determination of phosphonate in freshwater samples. For samples in coastal areas, estuaries and other areas, there are different levels of salt, which will have a certain impact on the determination results.

Images