Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork

A technology of florfenicol and florfenicol, applied in the field of veterinary drug residue detection, can solve problems such as no reports yet, and achieve the effects of good reproducibility, high analysis efficiency and moderate retention time

Inactive Publication Date: 2019-04-19
YANGZHOU UNIV
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, domestic and foreign detection methods for thiamphenicol, florfenicol and its metabolite florfenicol amine residues include microbial method, immunological detection method, gas chromatography, high performance liquid chromatography, gas chromatography-tandem Mass spectrometry and liquid chromatography-tandem mass spectrometry, etc., but ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) simultaneously detects thiamphenicol, florfenicol and their metabolites in poultry eggs, poultry meat and pork The method of florfenicol amine residue has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork
  • Analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork
  • Analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0034] The present invention will be described in further detail below in conjunction with specific examples and with reference to data. These examples are only for illustration of the present invention and do not limit the scope of the present invention in any way.

[0035] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art. The sources and trade names of the reagents used, as well as those whose components must be listed, are indicated when they appear for the first time, and the same reagents used thereafter are the same as those indicated for the first time unless otherwise specified.

[0036] 1. Breeding and sample collection of experimental livestock and poultry

[0037]30 28-week-old egg-laying Jinghai yellow chickens (Jiangsu Jinghai Poul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of veterinary drug residue detection, in particular to an analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork. The analysis method includes steps of 1), extracting and purifying poultry egg, poultry meat and pork samples; 2), carrying out ultrahigh-performance liquid chromatography-fluorescence detection. The ultrahigh-performance liquid chromatography-fluorescence detection includes a ultrahigh-performance liquid chromatography conditions and b fluorescence detection. The ultrahigh-performance liquid chromatography conditions include that aqueous solution with 0.005 mol / L of NaH2PO4, 0.003 mol / L of sodium lauryl sulfate and 0.05% of triethylamine is used as mobile phase A, and acetonitrile is used as mobile phase B. The analysis method has the advantages that the residues of the thiamphenicol, the florfenicol and the florfenicol amine in thepoultry eggs (chicken eggs, duck eggs, goose eggs, pigeon eggs and quail eggs), the poultry meat (chicken, duck meat and goose meat) and the pork can be efficiently and accurately simultaneously detected by the aid of the analysis method, and the florfenicol amine is metabolite of the florfenicol; the requirements of the Ministry of Agriculture of China, the FDA (Food and Drug Administration) ofthe America and the European Union (EU) on methods for detecting veterinary drug residues can be met.

Description

technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to an efficient and simultaneous detection of multiple residues of thiamphenicol, florfenicol and florfenicol amine in poultry eggs, poultry meat and pork. Background technique [0002] At present, domestic and foreign detection methods for thiamphenicol, florfenicol and its metabolite florfenicol amine residues include microbial method, immunological detection method, gas chromatography, high performance liquid chromatography, gas chromatography-tandem Mass spectrometry and liquid chromatography-tandem mass spectrometry, etc., but ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) simultaneously detects thiamphenicol, florfenicol and their metabolites in poultry eggs, poultry meat and pork The method of florfenicol amine residue has not been reported yet. Therefore, the present invention establishes for the first time a UPLC-FLD dete...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 谢恺舟王波赵霞谢星庞茂达王雅娟郭亚文卜晓娜刘楚君刁志祥张培杨王旭堂陈长宽张跟喜张涛戴国俊王冉卜仕金王金玉
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com