Analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork

Abstract

The invention relates to the field of veterinary drug residue detection, in particular to an analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork. The analysis method includes steps of 1), extracting and purifying poultry egg, poultry meat and pork samples; 2), carrying out ultrahigh-performance liquid chromatography-fluorescence detection. The ultrahigh-performance liquid chromatography-fluorescence detection includes a ultrahigh-performance liquid chromatography conditions and b fluorescence detection. The ultrahigh-performance liquid chromatography conditions include that aqueous solution with 0.005 mol / L of NaH2PO4, 0.003 mol / L of sodium lauryl sulfate and 0.05% of triethylamine is used as mobile phase A, and acetonitrile is used as mobile phase B. The analysis method has the advantages that the residues of the thiamphenicol, the florfenicol and the florfenicol amine in thepoultry eggs (chicken eggs, duck eggs, goose eggs, pigeon eggs and quail eggs), the poultry meat (chicken, duck meat and goose meat) and the pork can be efficiently and accurately simultaneously detected by the aid of the analysis method, and the florfenicol amine is metabolite of the florfenicol; the requirements of the Ministry of Agriculture of China, the FDA (Food and Drug Administration) ofthe America and the European Union (EU) on methods for detecting veterinary drug residues can be met.

Description

technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to an efficient and simultaneous detection of multiple residues of thiamphenicol, florfenicol and florfenicol amine in poultry eggs, poultry meat and pork. Background technique [0002] At present, domestic and foreign detection methods for thiamphenicol, florfenicol and its metabolite florfenicol amine residues include microbial method, immunological detection method, gas chromatography, high performance liquid chromatography, gas chromatography-tandem Mass spectrometry and liquid chromatography-tandem mass spectrometry, etc., but ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) simultaneously detects thiamphenicol, florfenicol and their metabolites in poultry eggs, poultry meat and pork The method of florfenicol amine residue has not been reported yet. Therefore, the present invention establishes for the first time a UPLC-FLD dete...

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Problems solved by technology

[0002] At present, domestic and foreign detection methods for thiamphenicol, florfenicol and its metabolite florfenicol amine residues include microbial method, immunological detection method, gas chromatography, high performance liquid chromatography, gas chromatography-tandem Mass spectrometry and liquid chromatography-tandem mass spectrometry, etc., but ultra-high performance liquid chromatography-fluorescence detection (UPLC-FLD) simultaneously detects thiamphenicol, florfenicol and their metabolites in poultry eggs, poultry meat and pork The method of florfenicol amine residue has not been reported yet

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