Sensitive capillary electrophoresis methods for detecting melamine in formula milk powder

A capillary electrophoresis and melamine technology, applied in the field of analysis and detection, can solve problems such as low detection sensitivity, and achieve the effects of low analysis cost, sensitive detection effect and environmental friendliness

Inactive Publication Date: 2012-07-11
JIANGNAN UNIV
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Problems solved by technology

Among these methods, some methods require more complex sample pretreatment process to remove the influence of the matrix in the sample and special packed columns, and some methods have relatively low detection sensitivity

Method used

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  • Sensitive capillary electrophoresis methods for detecting melamine in formula milk powder
  • Sensitive capillary electrophoresis methods for detecting melamine in formula milk powder
  • Sensitive capillary electrophoresis methods for detecting melamine in formula milk powder

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with specific examples.

[0021] The required instruments are: CAPEL105 high-efficiency capillary electrophoresis system, equipped with ultraviolet detector; uncoated quartz capillary (Φ75μm×65cm, effective length 50cm).

[0022] Reagents required:

[0023] Melamine standard, sodium dodecyl sulfate (SDS), sodium dihydrogen phosphate (NaH 2 PO 4 ), phosphoric acid, acetonitrile, methanol, 0.1mol / L HCl, 0.1mol / L NaOH, ultrapure water, and the buffer solution and sample solution used were all filtered through a 0.45 μm filter membrane before use.

[0024] 1) Determination of the best detection conditions

[0025] (1) Preparation of running solution and sample matrix

[0026] Prepare the concentration of 150mmol / L NaH with ultrapure water 2 PO 4 And 150mmol / L sodium lauryl sulfate mother liquor;

[0027] a. Use a concentration of 150mmol / L NaH 2 PO 4 And 150mmol / L sodium lauryl sulfate mother liqu...

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Abstract

The invention belongs to the technical field of analysis and detection and discloses two sensitive capillary electrophoresis methods for detecting melamine in a formula milk powder. FASS-CZE method and FASS-sweep MEKC method are adopted to detect melamine in a formula milk powder. By the adoption of the two methods, the melamine enrichment sensitivity is raised by 2-3 orders of magnitude in comparison with that by a common capillary zone electrophoresis (CZE) method, and detection limits are respectively reduced to 2.8 microgram / kg and 1.8 microgram / kg. By the adoption of electrokinetic injection under low electroosmotic flow, the method has selectivity of a sample which enters into the capillary, is less affected by sample matrix, requires no complex sample pretreatment processes in methods such as liquid chromatogram and the like, is suitable for detection of melamine in a plurality of samples and has high sensitivity; detection limit is reduced by two to three orders of magnitude in comparison with that in present liquid chromatogram and fluorescence spectrum method; a buffer used is mainly an aqueous solution; a lot of organic solvents are not required; the invention has little pollution and is environmentally friendly; the amount of required solvent and sample is small; the price of the open hole capillary column used is cheap; and the analysis cost is low.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and discloses a method for detecting melamine in formula milk powder by adopting a simple sample pretreatment method combined with capillary electrophoresis on-line enrichment technology. The invention adopts the method of detecting melamine in the formula milk powder by using the field amplification sample introduction combined with the zonal capillary electrophoresis method and adopting the field amplification sample introduction combined with the micelle sweeping method to detect the melamine in the formula milk powder. Background technique [0002] Melamine is an important nitrogen-heterocyclic organic chemical raw material. Since melamine molecules contain a large amount of nitrogen, criminals add melamine to feed and dairy products in order to falsely increase protein content. , eggs and other daily necessities may contain more than the limit value (the limit value in eggs is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N1/28G01N27/453
Inventor 陈新刘瑛商少明高海燕
Owner JIANGNAN UNIV
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