A method for the detection of common phosphonates in water with different salinities

Abstract

The invention relates to a detection method applicable to common phosphonate esters in water bodies with different salinities, and relates to a detection method for phosphonate esters in water bodies. A detection method suitable for common phosphonate esters in different salinity water bodies is provided, which has the advantages of simple operation, low requirements on instruments and the ability to simultaneously determine four phosphonate esters. A method for separating and measuring the concentration of common phosphonates in water—glyphosate, glufosinate, and aminomethylphosphonic acid 2-aminoethylphosphonic acid by using high-performance liquid chromatography fluorescence method. The specific steps are as follows: using fluorenyl methaneoxycarbonyl chloride as a derivatizing agent to react with phosphonate, and the reaction product enters high performance liquid chromatography for separation and determination after being filtered. It has the advantages of simple operation, low requirements for instruments, short sample determination time, and simultaneous determination of four phosphonate esters, etc., and can be applied to the determination of phosphonate esters in water bodies with different salinities.

Description

technical field

[0001] The invention relates to a method for detecting phosphonate in water, in particular to a method for detecting common phosphonate in water with different salinities. Background technique

[0002] Organophosphorus is one of the important forms of phosphorus in the ocean and plays a very important role in marine biogeochemical cycles. As a photolysis-resistant, high-temperature-resistant chemically stable C-P bond organic phosphorus, phosphonate is still relatively lacking in various aspects of research. In recent years, C-P bond organophosphorus pesticides represented by glyphosate and glufosinate-ammonium have been widely used all over the world. Accumulation poses a certain threat to environmental safety and human health. On the other hand, biogenic phosphonates represented by 2-aminoethylphosphonic acid widely exist in lower organisms. 2-Aminoethylphosphonic acid can be used as a potential phosphorus source for some organisms in aquatic environment...

Examples

Embodiment 1

[0030] Using the present invention to establish a working curve for a phosphonate with a salinity of 10.

[0031] Use artificial seawater with a salinity of 10 to prepare mixed standard solutions containing four kinds of phosphonate esters with 5 different concentration gradients, which are standard solution 1: glyphosate 80.0 μg / L, glufosinate Methylphosphonic acid 20.0μg / L, 2-aminoethylphosphonic acid 150μg / L; standard solution 2: glyphosate 160μg / L, glufosinate-ammonium 100μg / L, aminomethylphosphonic acid 40.0μg / L, 2- Aminoethylphosphonic acid 300μg / L; standard solution 3: glyphosate 240μg / L, glufosinate-ammonium 150μg / L, aminomethylphosphonic acid 60.0μg / L, 2-aminoethylphosphonic acid 450μg / L; standard solution 4: glyphosate 320μg / L, glufosinate-ammonium 200μg / L, aminomethylphosphonic acid 80.0μg / L, 2-aminoethylphosphonic acid 600μg / L; standard solution 5: glyphosate 400μg / L, glufosinate-ammonium Phosphine 250 μg / L, aminomethylphosphonic acid 100 μg / L, 2-aminoethylphospho...

Embodiment 2

[0035] The invention is used to determine the contents of four kinds of phosphonates in seawater biological culture samples.

technical field

[0001] The invention relates to a method for detecting phosphonate in water, in particular to a method for detecting common phosphonate in water with different salinities. Background technique

[0002] Organophosphorus is one of the important forms of phosphorus in the ocean and plays a very important role in marine biogeochemical cycles. As a photolysis-resistant, high-temperature-resistant chemically stable C-P bond organic phosphorus, phosphonate is still relatively lacking in various aspects of research. In recent years, C-P bond organophosphorus pesticides represented by glyphosate and glufosinate-ammonium have been widely used all over the world. Accumulation poses a certain threat to environmental safety and human health. On the other hand, biogenic phosphonates represented by 2-aminoethylphosphonic acid widely exist in lower organisms. 2-Aminoethylphosphonic acid can be used as a potential phosphorus source for some organisms in aquatic environment...

Examples

Embodiment 1

[0030] Using the present invention to establish a working curve for a phosphonate with a salinity of 10.

[0031] Use artificial seawater with a salinity of 10 to prepare mixed standard solutions containing four kinds of phosphonate esters with 5 different concentration gradients, which are standard solution 1: glyphosate 80.0 μg / L, glufosinate Methylphosphonic acid 20.0μg / L, 2-aminoethylphosphonic acid 150μg / L; standard solution 2: glyphosate 160μg / L, glufosinate-ammonium 100μg / L, aminomethylphosphonic acid 40.0μg / L, 2- Aminoethylphosphonic acid 300μg / L; standard solution 3: glyphosate 240μg / L, glufosinate-ammonium 150μg / L, aminomethylphosphonic acid 60.0μg / L, 2-aminoethylphosphonic acid 450μg / L; standard solution 4: glyphosate 320μg / L, glufosinate-ammonium 200μg / L, aminomethylphosphonic acid 80.0μg / L, 2-aminoethylphosphonic acid 600μg / L; standard solution 5: glyphosate 400μg / L, glufosinate-ammonium Phosphine 250 μg / L, aminomethylphosphonic acid 100 μg / L, 2-aminoethylphospho...

Embodiment 2

[0035] The invention is used to determine the contents of four kinds of phosphonates in seawater biological culture samples.

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