Large-volume flow-through cell for liquid chromatography fluorescence detector

A fluorescence detector and liquid chromatography technology, used in instruments, measuring devices, scientific instruments, etc., can solve problems such as unfavorable separation of mixed components, and achieve the effect of increasing the absolute number of fluorescent molecules, avoiding severe peak broadening, and high sensitivity.

CN104749299AActive Publication Date: 2015-07-01DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

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Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date: 2015-07-01

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Abstract

The invention provides a large-volume flow-through cell for a liquid chromatography fluorescence detector, wherein the large-volume flow-through cell is composed of an inlet guide tube, an inlet connector, a quartz tube, an outlet connector and an outlet guide tube. The inlet connector and the outlet connector are cylindrical in shape, the lower ends of the inlet connector and the outlet connector are each provided with a coaxial groove, and each groove is internally provided with a coaxial through hole; the upper ends of the inlet connector and the outlet connector are each provided with a coaxial inverted circular truncated cone shaped groove, the lower end face of each inverted circular truncated cone shaped groove is provided with a cylindrical groove, and the inner diameter of each cylindrical groove is the same as the diameter of the lower end face of the inverted circular truncated cone shaped groove and is greater than the diameter of the inner through hole of each groove. The outer wall faces of the upper ends of the inlet connector and the outlet connector are each sunken inwards to form an annular step along the radial direction, and the upper ends are respectively inserted into two ends of the large-inner-diameter quartz tube. A liquid channel having three-level inner diameter gradually increased is formed inside each of the inlet connector and the outlet connector, the problem of peak broadening caused by the increase of the cell volume is solved, and high sensitivity and high separation degree are obtained.

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Description

technical field

[0001] The patent of the present invention relates to the technical field of liquid chromatography detectors, more specifically, to a flow cell for fluorescence detection. Background technique

[0002] Liquid chromatography (LC), as a high-efficiency separation technique, is widely used in the separation and analysis of mixed substances. The detector is one of the key components of the liquid chromatography system. Fluorescence detector (FLD) is a high-sensitivity detector, combined with liquid chromatography and fluorescence detector, widely used in the detection of trace biological and environmental substances in complex mixed samples. The flow cell is one of the core components of the fluorescence detector, and its main function is to transmit the mobile phase and provide the fluorescence detection area. The excitation light is irradiated on the mobile phase in the flow cell, and when a fluorescent substance flows through, the substance is excited to emi...

Claims

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