Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Large-volume flow-through cell for liquid chromatography fluorescence detector

A fluorescence detector and liquid chromatography technology, used in instruments, measuring devices, scientific instruments, etc., can solve problems such as unfavorable separation of mixed components, and achieve the effect of increasing the absolute number of fluorescent molecules, avoiding severe peak broadening, and high sensitivity.

Active Publication Date: 2015-07-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF10 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the larger the volume of the flow cell, the greater the broadening of the peaks, which is not conducive to the separation of mixed components

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Large-volume flow-through cell for liquid chromatography fluorescence detector
  • Large-volume flow-through cell for liquid chromatography fluorescence detector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 As shown, a large-volume flow cell for a liquid chromatography fluorescence detector is composed of an inlet guide tube 1 , an inlet connector 2 , a quartz tube 3 , an outlet connector 4 and an outlet guide tube 5 . The inlet joint 2 and the outlet joint 4 are made of aluminum alloy, the surface is oxidized and blackened, and the lower ends are provided with coaxial grooves, and coaxial through holes are arranged in the grooves. The inlet guide pipe 1 and the outlet guide pipe 5 are stainless steel pipes, which are respectively placed in the lower end grooves of the inlet joint 2 and the outlet joint 4, and are fastened by glue. The inner diameter of the inlet guide pipe 1 is 0.4 mm, and the diameter of the through hole in the groove at the lower end of the inlet joint 2 is 0.5 mm. The inner diameter of the outlet guide pipe 5 is 0.5 mm, and the diameter of the through hole in the groove at the lower end of the outlet joint 4 is 1.0 mm.

[0042]The up...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Lengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a large-volume flow-through cell for a liquid chromatography fluorescence detector, wherein the large-volume flow-through cell is composed of an inlet guide tube, an inlet connector, a quartz tube, an outlet connector and an outlet guide tube. The inlet connector and the outlet connector are cylindrical in shape, the lower ends of the inlet connector and the outlet connector are each provided with a coaxial groove, and each groove is internally provided with a coaxial through hole; the upper ends of the inlet connector and the outlet connector are each provided with a coaxial inverted circular truncated cone shaped groove, the lower end face of each inverted circular truncated cone shaped groove is provided with a cylindrical groove, and the inner diameter of each cylindrical groove is the same as the diameter of the lower end face of the inverted circular truncated cone shaped groove and is greater than the diameter of the inner through hole of each groove. The outer wall faces of the upper ends of the inlet connector and the outlet connector are each sunken inwards to form an annular step along the radial direction, and the upper ends are respectively inserted into two ends of the large-inner-diameter quartz tube. A liquid channel having three-level inner diameter gradually increased is formed inside each of the inlet connector and the outlet connector, the problem of peak broadening caused by the increase of the cell volume is solved, and high sensitivity and high separation degree are obtained.

Description

technical field [0001] The patent of the present invention relates to the technical field of liquid chromatography detectors, more specifically, to a flow cell for fluorescence detection. Background technique [0002] Liquid chromatography (LC), as a high-efficiency separation technique, is widely used in the separation and analysis of mixed substances. The detector is one of the key components of the liquid chromatography system. Fluorescence detector (FLD) is a high-sensitivity detector, combined with liquid chromatography and fluorescence detector, widely used in the detection of trace biological and environmental substances in complex mixed samples. The flow cell is one of the core components of the fluorescence detector, and its main function is to transmit the mobile phase and provide the fluorescence detection area. The excitation light is irradiated on the mobile phase in the flow cell, and when a fluorescent substance flows through, the substance is excited to emi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/74
Inventor 关亚风耿旭辉刘洪鹏完玲中
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com