Amino functional group chiral compound resolution labeling with fluorescent derivatization reagent

A chiral compound, fluorescence derivatization technology, applied in the field of biological analysis, can solve the problem of not achieving complete chiral resolution, and achieve the effect of reliable fluorescent chiral derivatization reagents
CN106770838BActive Publication Date: 2019-05-14YANBIAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANBIAN UNIV
Publication Date
2019-05-14

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Abstract

The invention relates to an amino functional group chiral compound resolution mark band fluorescence derivatizing agent, in particular to a DBD-trans-2-methyl-L-proline (DBD-M-Pro) agent and a DBD-(2-succinimidoxy)-trans-2-methyl-L-proline (DBD-M-Pro) agent. The two agents contain benzofurazan as a fluorescence functional group and a chiral center structure, can carry out fluorescence marking on an amino functional group chiral compound and establishes an analysis method with high sensitivity and high selectivity in amino functional group chiral compound resolution by utilizing a liquid chromatography-fluorescence detection (LC-FL) technology. Since containing the fluorescence functional group self, the agent can remarkably improve the detection sensitivity in a fluorescence detector, can also carry out chiral resolution on the amino functional group chiral compound in reversed phase chromatography and supplies a novel high-sensitivity fluorescence chiral derivatizing agent for studying resolution of an amino functional group chiral metabolite and screening various disease chiral biomarkers.
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Description

technical field

[0001] The invention relates to the analysis of chiral metabolites containing amino functional groups in the field of biological analysis, in particular to novel high-sensitivity fluorescent derivatization reagents for chiral resolution of chiral compounds with amino functional groups. Background technique

[0002] Chirality is a fundamental feature of biological systems, especially in the field of pharmacy. In many cases, the biological activities of two isomeric drugs, such as potency and pharmacokinetics, sometimes differ significantly. Chirality is in turn an important feature of many endogenous metabolites. In order to accurately screen truly effective chiral biomarkers, liquid chromatography-fluorescence detection (LC-FL) technology is used to develop and establish a highly sensitive chiral metabolite separation and analysis method, which is useful for drug toxicity and mechanism research, disease It is of great significance for the elucidation of dia...

Claims

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