12 results about "Tetrodotoxin" patented technology

The invention relates to the technical field of medicines, in particular to application of tedizolid combined with fluconazole in preparation of antifungal drugs. The tedizolid and the fluconazole are combined for use, so that the antibacterial activity of the fluconazole on the candida albicans can be enhanced, a synergistic antifungal effect can be generated, and the drug resistance of the candida albicans on the fluconazole can be reversed.

The invention discloses a tetrodotoxin detection method based on a magnetic control micro-fluidic chip. The tetrodotoxin detection method comprises the following steps: preparing the magnetic control micro-fluidic chip; constructing a TTX specific capture probe; preparing a signal probe with catalytic activity; the method comprises the following steps: adding a sample to be detected and a capture probe into a chip capture cavity, and fully incubating magnetic microspheres and the sample through magnetic control driving to form a magnetic microsphere-TTX compound; washing and carrying out sandwich reaction to form an aptamer-TTX-antibody-APMS nano enzyme sandwich compound; the sandwich compound reacts with a TMB-H2O2 chromogenic substrate, and quantitative or qualitative analysis of TTX is realized by detecting a chromogenic signal. According to the present invention, the novel tetrodotoxin rapid detection technology based on the aptamer and antibody sandwich immunoassay, the noble metal nano-enzyme signal amplification and the magnetic control microfluidic chip is provided, and the requirements of high sensitivity, simplicity and convenience of the on-site food safety in-situ detection are met.

Provided are a tetrodotoxin conjugate, a method for preparing same, and use thereof. A carboxyl-containing macromolecular compound and tetrodotoxin are used as the starting materials, and after a catalyst and a solvent are added, the tetrodotoxin conjugate is obtained by means of a coupling reaction. The carboxyl-containing macromolecular compound is selected from carboxylated dextran, carboxylated β-cyclodextrin, alginic acid, and polyacrylic acid. The tetrodotoxin conjugate can be used in the preparation of a sodium channel blocker and a medicament for treating neuropathic pain caused by chemotherapy, and possesses an analgesic effect associated with tetrodotoxin (TTX). In addition, the therapeutic window is increased, thereby greatly improving the safety.

A process for extracting TTX from biological material containing TTX, wherein the process comprises steps of extracting with a first solvent, concentrating, washing with a second solvent, concentrating again and purifying by chromatography.

Relates to a compound with an inhibition effect on various sodium ion channels or a pharmaceutically acceptable derivative and a pharmaceutical composition thereof, and application of the compound or the pharmaceutically acceptable derivative and the pharmaceutical composition as a sodium ion channel blocker.

The invention relates to a colloidal gold immunochromatography detection method for tetrodotoxin in blood and a kit thereof.The method comprises the following steps that 1, an extracting agent is added into a blood sample, vortex oscillation and centrifugation are conducted, supernate is taken, the extracting agent is a mixed solution of acetic acid and acetonitrile, and the extracting agent is a mixed solution of ethyl acetate and acetonitrile; the adding amount of the extracting agent is 390-410 [mu] L per 200 [mu] L of blood sample; 2, carrying out nitrogen blowing drying on the supernate obtained in the step 1; 3, a resolvent is added into the residues obtained after nitrogen blowing drying, a solution to be detected is obtained after redissolving, the resolvent is a PBS buffer solution, and the adding amount of the resolvent is 145-160 microliters. According to the method, key parameters of the blood sample pretreatment step are optimized, blood matrix interference is effectively removed, the colloidal gold immunochromatography process is ensured to be smoothly carried out, and therefore rapid, sensitive and reliable qualitative screening of tetrodotoxin in blood is achieved.

The invention relates to the field of medicines, the technical field of biology and the technical field of preparation manufacturing, in particular to a pharmaceutical composition containing tetrodotoxin as well as a transdermal patch, a preparation method and application of the pharmaceutical composition. The pharmaceutical composition provided by the invention comprises an active component, a pressure-sensitive adhesive and a cosolvent, the pressure-sensitive adhesive is prepared from at least one of Eudragit E100, Eudragit L100, Eudragit S100 and polysiloxane, and the pressure-sensitive adhesive is prepared from at least one of Eudragit E100, Eudragit L100, Eudragit S100 and polysiloxane; wherein the cosolvent comprises at least one of citric acid, acetic acid, malic acid or tartaric acid, preferably citric acid; the active component comprises tetrodotoxin or a pharmaceutically acceptable salt of the tetrodotoxin. The pharmaceutical composition provided by the invention has excellent stability, accumulative release rate and smooth release effect, and has unexpected technical effects.

The invention relates to a preparation method of a test strip for detecting tetrodotoxin based on a magnetic fluorescence signal probe and a Fab-tetrodotoxin-aptamer sandwich method. The preparation method comprises the following steps: preparing streptavidin magnetic beads with fluorescence signals through an amide reaction; the preparation method comprises the following steps: shearing an anti-tetrodotoxin monoclonal antibody stored in a laboratory by using papain to obtain a Fab fragment, biotinylating the Fab fragment, and then coupling the biotinylated Fab fragment with streptavidin magnetic beads with fluorescence signals through high affinity between biotin and streptavidin to obtain the magnetic fluorescence signal probe. The prepared signal probe has three functions: a matrix is separated and toxin is enriched through magnetic separation, the magnetic beads simultaneously generate a colorimetric signal and a fluorescence signal, and a biotin-streptavidin system amplifies a detection signal. In addition, the structure of the existing aptamer is optimized, and the obtained truncated aptamer is used for forming a detection line of the test strip. Tetrodotoxin is detected by using an antibody-toxin-aptamer sandwich method principle, so that non-specific binding can be effectively reduced, the anti-interference capability is improved, rapid qualitative and quantitative detection of a sample can be realized, and the kit has a good application prospect.

4-bromobenzyl alcohol is used as a raw material, an initial chiral center and a key diene structure (a compound 8) are efficiently constructed through a hectogram-scale enzymatic dearomatization dihydroxylation reaction, and then a cycloaddition reaction, a fragmentation reaction, a nitrile group reduction reaction and a cyclic guanidyl formation reaction are performed to prepare the compound 1 (tetrodotoxin). The preparation process provided by the invention starts from cheap and easily available 4-bromobenzyl alcohol, realizes 15-step large-scale synthesis through strategic combination of biological catalysis and chemical synthesis, and shows excellent industrial application prospects.

A process for extracting TTX from biological material containing TTX, wherein the process comprises steps of extracting with a first solvent, concentrating, washing with a second solvent, concentrating again and purifying by chromatography.

The application discloses a method for preparing tetrodotoxin monomers and active chiral isomers by high performance liquid chromatography, and belongs to the technical field of medicines. The technical problem to be solved is to provide a method for separating and preparing tetrodotoxin TTX, active chiral isomer M002 and active chiral isomer 4,9-dehydrated-TTX. The technical solution is characterized in that the tetrodotoxin TTX, active chiral isomer M002 and active chiral isomer 4,9-dehydrated-TTX are separated and prepared by high performance liquid chromatography. The chromatographic conditions of the high performance liquid chromatography are as follows: a C18 chromatographic column with a filler particle size of 5 microns; a mobile phase A of 0.01%-0.2% trifluoroacetic acid aqueous solution; a mobile phase B of an alcohol solution; and gradient elution.

The invention relates to an aptamer for specifically recognizing tetrodotoxin and application of the aptamer, and belongs to the technical field of detection. The cyclic divalent aptamer disclosed by the invention has good affinity and specificity and good thermal stability, is not interfered by impurities such as ion strength and protein in globefish meat and serum during tetrodotoxin detection, and has high conformational stability. Based on the annular bivalent aptamer, the invention further designs an aptamer sensor based on a 3D-DNA walker, the aptamer sensor can specifically detect low-concentration tetrodotoxin, the detection limit is 0.02 ng / mL, the detection time is 1 h, and the detection sensitivity is high. Other marine toxins such as gonyautoxin (GTX), dinophysiotoxin (DTX), sea anemone toxin II (ATX-II), anabaena toxin-a (ATX-a) and microcystin (MC-LR) cannot be detected, and the specificity is good.