24 results about "Tetrodotoxin" patented technology

The invention relates to the technical field of medicines, in particular to application of tedizolid combined with fluconazole in preparation of antifungal drugs. The tedizolid and the fluconazole are combined for use, so that the antibacterial activity of the fluconazole on the candida albicans can be enhanced, a synergistic antifungal effect can be generated, and the drug resistance of the candida albicans on the fluconazole can be reversed.

The invention relates to the technical field of biological detection, and discloses a tetrodotoxin colloidal gold detection card, which comprises a detection box used for packaging a detection assembly, the display groove is formed in the detection box and is used for observing a detection result; the liquid dropping groove is positioned at one end of the detection box and is used for accommodating a to-be-detected sample; and the detection paper is arranged in the detection box, comprises a nitrocellulose membrane, an adsorption pad and a diffusion pad, is provided with a detection area and a control area, and is used for displaying a detection result of tetrodotoxin. By optimizing the size of the gold nanoparticles and an antibody labeling process, the sensitivity and the specificity of the detection card are remarkably improved, and the low-concentration tetrodotoxin can be rapidly and accurately detected. Due to the reasonable particle size and the application of the stabilizer, non-specific adsorption is reduced, the false positive rate is reduced, the reliability and consistency of detection results are ensured, clear detection results can be obtained in a shorter time, and the accuracy of rapid judgment in field application is ensured.

A method for extracting TTX from a biological material containing TTX, wherein the method comprises the steps of extraction with a first solvent, concentration, washing with a second solvent, re-concentration and purification by chromatography.

The present invention provides a method for electrochemically detecting tetrodotoxin based on Ag@copper oxide nanoparticles, belonging to the technical field of electrochemical analysis. The present invention is based on AgNPs, and a Cu2O shell is grown around it to synthesize Ag@Cu2ONPs. The prepared Ag@Cu2ONPs have two stable electrochemical signals, at -0.13V and 0.17V respectively. The Ag@Cu2ONPs modified with nucleic acid aptamers are incubated with the substrate material (MXenes NSs) to form an assembly. When tetrodotoxin is present, the Ag@Cu2ONPs are detached from the substrate material, resulting in the weakening of the two electrochemical signals. Both signals of the electrochemical sensor linearly increase with the increase of the logarithm of the concentration of tetrodotoxin, realizing sensitive and accurate detection of tetrodotoxin.

The invention belongs to the field of bacterial detection of environmental microorganisms, and particularly relates to a detection method for in-situ labeling of tetrodotoxin in bacteria, which comprises the following steps: preparing a slice by using bacteria fixed by a solution containing methanol or ethanol, then carrying out pre-treatment of de-embedding and non-specific sealing, finally carrying out antigen-antibody reaction, and carrying out microscopic examination on a labeling signal by an electron microscope, so as to obtain the tetrodotoxin in-situ labeling detection of the tetrodotoxin in the bacteria. The qualitative and / or quantitative determination of the intracellular tetrodotoxin of the bacteria is realized. The method is high in specificity, good in repeatability and strong in observability, is used for detecting TTX-producing bacteria in the environment, and provides an effective and reliable means for researching the synthesis mechanism of the toxin in the bacteria.

The invention relates to the technical field of biological adsorption materials, in particular to modified peptidoglycan with an efficient adsorption effect and a preparation method and application of the modified peptidoglycan. The preparation method comprises the steps of grafting and modifying carboxyl / hydroxyl of peptidoglycan, so that multi-site and multi-mode functional modification on the surface of the peptidoglycan is realized. The physicochemical properties of functional groups on the surface of peptidoglycan are changed through modification treatment, the density and electronegativity of carboxyl and hydroxyl functional groups on the surface of peptidoglycan are improved, the hydrogen-bond interaction and electrostatic adsorption capacity between the peptidoglycan and biotoxin are enhanced, and the adsorption efficiency is improved. In addition, the peptidoglycan is subjected to carboxyl and hydroxyl modification at the same time, and the tetrodotoxin adsorption effect of the peptidoglycan can be further improved. The technology provides a new strategy for functionalization of natural polymer materials, has good theoretical popularization value and application prospects, and is suitable for the fields of food safety, removal and purification of biotoxins and the like.

The invention discloses a preparation method of a tetrodotoxin (TTX) external preparation and an application of the tetrodotoxin (TTX) external preparation in treatment of postherpetic neuralgia. The method comprises the following steps: preparing external preparations with different TTX contents, establishing a postherpetic neuralgia animal model, and verifying the treatment effect of the external preparations on the postherpetic neuralgia through animal experiments. TTX is an amino perhydroquinazoline type small molecule compound. A novel preparation is developed on the basis that tetrodotoxin is a voltage-gated sodium ion channel inhibitor. Experiments prove that by inhibiting the tetrodotoxin sensitive voltage-gated sodium ion channel Nav1.7, the tetrodotoxin external preparation shows remarkable postherpetic neuralgia resistance activity, and can be researched and developed as a postherpetic neuralgia medicine.

The invention discloses a tetrodotoxin detection method based on a magnetic control micro-fluidic chip. The tetrodotoxin detection method comprises the following steps: preparing the magnetic control micro-fluidic chip; constructing a TTX specific capture probe; preparing a signal probe with catalytic activity; the method comprises the following steps: adding a sample to be detected and a capture probe into a chip capture cavity, and fully incubating magnetic microspheres and the sample through magnetic control driving to form a magnetic microsphere-TTX compound; washing and carrying out sandwich reaction to form an aptamer-TTX-antibody-APMS nano enzyme sandwich compound; the sandwich compound reacts with a TMB-H2O2 chromogenic substrate, and quantitative or qualitative analysis of TTX is realized by detecting a chromogenic signal. According to the present invention, the novel tetrodotoxin rapid detection technology based on the aptamer and antibody sandwich immunoassay, the noble metal nano-enzyme signal amplification and the magnetic control microfluidic chip is provided, and the requirements of high sensitivity, simplicity and convenience of the on-site food safety in-situ detection are met.

Provided are a tetrodotoxin conjugate, a method for preparing same, and use thereof. A carboxyl-containing macromolecular compound and tetrodotoxin are used as the starting materials, and after a catalyst and a solvent are added, the tetrodotoxin conjugate is obtained by means of a coupling reaction. The carboxyl-containing macromolecular compound is selected from carboxylated dextran, carboxylated β-cyclodextrin, alginic acid, and polyacrylic acid. The tetrodotoxin conjugate can be used in the preparation of a sodium channel blocker and a medicament for treating neuropathic pain caused by chemotherapy, and possesses an analgesic effect associated with tetrodotoxin (TTX). In addition, the therapeutic window is increased, thereby greatly improving the safety.

The invention discloses a cyclohexadiene derivative and a preparation method thereof. According to the method, the chemical synthesis blank of the tetrodotoxin intermediate 10 is filled, and the cyclohexadiene derivative is obtained by taking cheap and easily available D-ribose as a raw material through the steps of hydroxyl protection, alkylation, oxidation, addition, cyclization, elimination, deprotection and the like. The preparation method is high in atom utilization degree and high in reaction specificity of each step, compared with enzyme catalysis, the research and development cost is reduced, complex separation and purification operation is avoided, and the preparation method has industrial amplification prospects.

A process for extracting TTX from biological material containing TTX, wherein the process comprises steps of extracting with a first solvent, concentrating, washing with a second solvent, concentrating again and purifying by chromatography.

Relates to a compound with an inhibition effect on various sodium ion channels or a pharmaceutically acceptable derivative and a pharmaceutical composition thereof, and application of the compound or the pharmaceutically acceptable derivative and the pharmaceutical composition as a sodium ion channel blocker.

The invention relates to a colloidal gold immunochromatography detection method for tetrodotoxin in blood and a kit thereof.The method comprises the following steps that 1, an extracting agent is added into a blood sample, vortex oscillation and centrifugation are conducted, supernate is taken, the extracting agent is a mixed solution of acetic acid and acetonitrile, and the extracting agent is a mixed solution of ethyl acetate and acetonitrile; the adding amount of the extracting agent is 390-410 [mu] L per 200 [mu] L of blood sample; 2, carrying out nitrogen blowing drying on the supernate obtained in the step 1; 3, a resolvent is added into the residues obtained after nitrogen blowing drying, a solution to be detected is obtained after redissolving, the resolvent is a PBS buffer solution, and the adding amount of the resolvent is 145-160 microliters. According to the method, key parameters of the blood sample pretreatment step are optimized, blood matrix interference is effectively removed, the colloidal gold immunochromatography process is ensured to be smoothly carried out, and therefore rapid, sensitive and reliable qualitative screening of tetrodotoxin in blood is achieved.

The invention provides a processing technology for globefish food, and relates to the technical field of food processing, and the processing technology comprises the following steps: S1, living body selection: selecting a living globefish of a predetermined variety in a predetermined breeding environment, and checking the selected globefish; s2, slaughtering; s3, detoxication treatment: removing liver, ovary, skin and toxin-containing parts of the globefish by using a three-step detoxication method; s4, cutting treatment, wherein the globefish subjected to detoxification treatment is cut into blocks and peeled; s5, performing detoxification again; s6, processing operation: putting the pufferfish blocks into processing equipment according to needs to prepare the required pufferfish food; and S7, packaging and storing. According to the invention, the residual quantity of tetrodotoxin is controlled below the safety standard through a dual detoxication process of S3 detoxication treatment and S5 secondary detoxication, so that the health of consumers is guaranteed; according to the invention, the taste of the globefish food is improved through the operations of slaughtering in S2 and processing in S6; and large-scale production is easy, the production efficiency is improved, and the cost is reduced.

The invention relates to the field of medicines, the technical field of biology and the technical field of preparation manufacturing, in particular to a pharmaceutical composition containing tetrodotoxin as well as a transdermal patch, a preparation method and application of the pharmaceutical composition. The pharmaceutical composition provided by the invention comprises an active component, a pressure-sensitive adhesive and a cosolvent, the pressure-sensitive adhesive is prepared from at least one of Eudragit E100, Eudragit L100, Eudragit S100 and polysiloxane, and the pressure-sensitive adhesive is prepared from at least one of Eudragit E100, Eudragit L100, Eudragit S100 and polysiloxane; wherein the cosolvent comprises at least one of citric acid, acetic acid, malic acid or tartaric acid, preferably citric acid; the active component comprises tetrodotoxin or a pharmaceutically acceptable salt of the tetrodotoxin. The pharmaceutical composition provided by the invention has excellent stability, accumulative release rate and smooth release effect, and has unexpected technical effects.

The invention belongs to the technical field of surface enhanced Raman spectroscopy, and particularly relates to a cDNA conformation rearrangement-based tetrodotoxin SERS (Surface Enhanced Raman Scattering) detection method. The preparation method comprises the following steps: firstly, preparing MIL-101, and further preparing an SERS enhanced substrate MIL-101 (at) Au NPs solution; the method comprises the following steps: preparing an SERS probe solution based on MB-labeled cDNA and an MIL-101 at Au NPs solution, triggering conformation rearrangement of the MB-labeled cDNA on an MIL-101 at Au NPs substrate through specific binding of a TTX aptamer and TTX, constructing an SERS detection system, and forming an aptamer-TTX-cDNA cascade verification mode; according to the system, a Raman signal molecule MB at the tail end of a cDNA chain is close to the surface of an SERS substrate by utilizing conformation rearrangement of the cDNA chain, rapid and high-sensitivity detection of TTX is realized through a local electromagnetic field coupling effect, and the system has a wide market application prospect.

The invention relates to a preparation method of a test strip for detecting tetrodotoxin based on a magnetic fluorescence signal probe and a Fab-tetrodotoxin-aptamer sandwich method. The preparation method comprises the following steps: preparing streptavidin magnetic beads with fluorescence signals through an amide reaction; the preparation method comprises the following steps: shearing an anti-tetrodotoxin monoclonal antibody stored in a laboratory by using papain to obtain a Fab fragment, biotinylating the Fab fragment, and then coupling the biotinylated Fab fragment with streptavidin magnetic beads with fluorescence signals through high affinity between biotin and streptavidin to obtain the magnetic fluorescence signal probe. The prepared signal probe has three functions: a matrix is separated and toxin is enriched through magnetic separation, the magnetic beads simultaneously generate a colorimetric signal and a fluorescence signal, and a biotin-streptavidin system amplifies a detection signal. In addition, the structure of the existing aptamer is optimized, and the obtained truncated aptamer is used for forming a detection line of the test strip. Tetrodotoxin is detected by using an antibody-toxin-aptamer sandwich method principle, so that non-specific binding can be effectively reduced, the anti-interference capability is improved, rapid qualitative and quantitative detection of a sample can be realized, and the kit has a good application prospect.

4-bromobenzyl alcohol is used as a raw material, an initial chiral center and a key diene structure (a compound 8) are efficiently constructed through a hectogram-scale enzymatic dearomatization dihydroxylation reaction, and then a cycloaddition reaction, a fragmentation reaction, a nitrile group reduction reaction and a cyclic guanidyl formation reaction are performed to prepare the compound 1 (tetrodotoxin). The preparation process provided by the invention starts from cheap and easily available 4-bromobenzyl alcohol, realizes 15-step large-scale synthesis through strategic combination of biological catalysis and chemical synthesis, and shows excellent industrial application prospects.

A process for extracting TTX from biological material containing TTX, wherein the process comprises steps of extracting with a first solvent, concentrating, washing with a second solvent, concentrating again and purifying by chromatography.

The application discloses a method for preparing tetrodotoxin monomers and active chiral isomers by high performance liquid chromatography, and belongs to the technical field of medicines. The technical problem to be solved is to provide a method for separating and preparing tetrodotoxin TTX, active chiral isomer M002 and active chiral isomer 4,9-dehydrated-TTX. The technical solution is characterized in that the tetrodotoxin TTX, active chiral isomer M002 and active chiral isomer 4,9-dehydrated-TTX are separated and prepared by high performance liquid chromatography. The chromatographic conditions of the high performance liquid chromatography are as follows: a C18 chromatographic column with a filler particle size of 5 microns; a mobile phase A of 0.01%-0.2% trifluoroacetic acid aqueous solution; a mobile phase B of an alcohol solution; and gradient elution.

The invention relates to an aptamer for specifically recognizing tetrodotoxin and application of the aptamer, and belongs to the technical field of detection. The cyclic divalent aptamer disclosed by the invention has good affinity and specificity and good thermal stability, is not interfered by impurities such as ion strength and protein in globefish meat and serum during tetrodotoxin detection, and has high conformational stability. Based on the annular bivalent aptamer, the invention further designs an aptamer sensor based on a 3D-DNA walker, the aptamer sensor can specifically detect low-concentration tetrodotoxin, the detection limit is 0.02 ng / mL, the detection time is 1 h, and the detection sensitivity is high. Other marine toxins such as gonyautoxin (GTX), dinophysiotoxin (DTX), sea anemone toxin II (ATX-II), anabaena toxin-a (ATX-a) and microcystin (MC-LR) cannot be detected, and the specificity is good.

The application belongs to the technical field of food safety detection, and particularly relates to a preparation method of a colorimetric-SERS dual-mode biosensor based on a hairpin probe and application of the colorimetric-SERS dual-mode biosensor in detection of tetrodotoxin. The specific steps are as follows: gold nanoparticles are prepared first, then the surface of the gold nanoparticles is modified; gold-silver core-shell nanoparticles are prepared and a hairpin probe is designed; finally, a colorimetric-SERS dual-mode biosensor based on the hairpin probe is constructed; the colorimetric-SERS dual-mode biosensor based on the hairpin probe constructed in the application is supplemented with Au@Ag NPs as a signal amplification element, high specificity and sensitivity of tetrodotoxin in food are realized, the detection limit of tetrodotoxin can reach 1.07 ng / mL and 0.09 ng / mL respectively, the colorimetric-SERS dual-mode biosensor does not need expensive instruments, is faster than traditional LC-MS, and has a good application prospect.