The present invention proposes a method for the industrialization of crude and pure toxins in live sea anemones, which uses the methods of starch coagulation preparation, coating, toxin collection, enzymolysis, centrifugation, nickel column chromatography, and ultrafiltration to remove impurities to obtain sea anemone protein toxoids Crude pure liquid. Compared with the prior art, the beneficial effect of the present invention is: without using protease or proteolytic agent, there are many types of sea anemone protein toxoids obtained; only sea anemone toxic substances are collected without collecting sea anemone tentacles, and the Sea anemone has little damage, its regeneration time is short, and its production sustainability is extremely advantageous, which provides conditions for the future industrial use of sea anemone toxins; the collected sea anemone toxins are less likely to be polluted by external proteins, and the protein isolated from the preliminary purification is only Create a good foundation for further purification of sea anemone toxin protein.