6 results about "Sea anemone" patented technology

This invention relates to the field of pharmaceutical science, specifically to a sea anemone polypeptide, its preparation method, and its applications. The sea anemone polypeptide has the N-terminal sequence YPDQKMFELFWLEENNA, a molecular weight of 6027 Da, and an isoelectric point of 7.25. Preparation process: Fresh sea anemone is precipitated with 10%-70% acetone. After dialysis to remove salt from the active component, it is purified by ion exchange chromatography, gel filtration chromatography, and HPLC to obtain a sea anemone polypeptide with a molecular weight of 6027 Da. Experiments on tumor cells confirm that the sea anemone polypeptide has a significant inhibitory effect on the growth of various tumor cells but no inhibitory effect on the growth of normal cells.

The invention relates to the technical field of marine product breeding, in particular to sea anemone breeding equipment and method.The equipment comprises a support, a temporary breeding cylinder, a circulating pipeline, a flow guide pipeline, a cutting breeding cylinder and a circulating pump, the support is fixed to the ground, and the temporary breeding cylinder and the cutting breeding cylinder are fixed to the support; a flow guide outlet in the side face of the temporary culture cylinder is connected with a flow guide inlet above the cutting propagation cylinder through a flow guide pipeline, a circulating outlet in the bottom of the cutting propagation cylinder is connected with a circulating inlet above the temporary culture cylinder through a circulating pipeline, the circulating pump is installed on the circulating pipeline, and control valves are installed on the circulating pipeline and the flow guide pipeline respectively. Through the steps of temporary culture, cutting and repairing growth, the sea anemone breeding device has the advantages of being simple in structure, high in breeding efficiency, good in breeding effect and the like.

The present application provides a kind of purple spot sea anemone polypeptide toxin HC-G02 and its synthesis method, application, its amino acid sequence is APCSGCYYQVGNECVYDKLKC-NH2. Linear peptide HC-G02 is synthesized by adopting polypeptide solid-phase synthesis method, and the purity of linear peptide after purification reaches more than 95%. Purple spot sea anemone oxidized peptide HC-G02 (the connection mode of disulfide bond is C1-C3, C2-C4) is obtained by site-directed oxidation. The present application is found through hot plate experiment test that purple spot sea anemone oxidized peptide HC-G02 has good analgesic effect on physical pain of mouse, and the pain threshold of mouse intraperitoneal injection oxidized peptide HC-G02 is gradually increased in 30min, and the pain threshold reaches the highest in 120min after administration. The analgesic effect of oxidized peptide HC-G02 at 50mg / kg is equivalent to the analgesic effect of tramadol at the same concentration, and the analgesic time is prolonged.

The present application provides a kind of zootoxin Ed79-WR of zonatus sea anemone polypeptide and its preparation method and application, its amino acid sequence is ACRDTYFASSC (Acm) KAAVDGDRC (tBu) RKSNYKTGC (Acm) AKT C (tBu) GYCT.The linear peptide Ed79-WR is synthesized by using polypeptide solid-phase synthesis method (SPPS) in the present application, the linear peptide is purified, then it is obtained by site-specific oxidation to contain three disulfide bonds Oxidized peptide Ed79-WR (the connection mode of disulfide bond is C1-C6, C2-C4, C3-C5), and the product is prepared after oxidation and folding Liquid phase purification, high performance liquid chromatography analysis and mass spectrometry identification, obtain zonatus sea anemone polypeptide Ed79-WR.The synthetic oxidized peptide Ed79-WR is tested by lethal experiment in the present application to the insecticidal effect of grass caterpillar, and the zonatus sea anemone polypeptide toxin Ed79-WR can be applied to the preparation of insecticide.

1. The name of the design product: powder puff (sea anemone). 2. The use of the design product: powder puff for use in making up. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view.

The invention relates to an aptamer for specifically recognizing tetrodotoxin and application of the aptamer, and belongs to the technical field of detection. The cyclic divalent aptamer disclosed by the invention has good affinity and specificity and good thermal stability, is not interfered by impurities such as ion strength and protein in globefish meat and serum during tetrodotoxin detection, and has high conformational stability. Based on the annular bivalent aptamer, the invention further designs an aptamer sensor based on a 3D-DNA walker, the aptamer sensor can specifically detect low-concentration tetrodotoxin, the detection limit is 0.02 ng / mL, the detection time is 1 h, and the detection sensitivity is high. Other marine toxins such as gonyautoxin (GTX), dinophysiotoxin (DTX), sea anemone toxin II (ATX-II), anabaena toxin-a (ATX-a) and microcystin (MC-LR) cannot be detected, and the specificity is good.