Model for detecting cell density of microalgae producing paralytic saxitoxin and as establishment method and application of model

Abstract

The invention discloses a model for detecting the cell density of microalgae producing paralytic shellfish toxin and an establishment method and application of the model. According to the method, three-dimensional fluorescence spectrum information of algae producing paralytic shellfish poison or algae not producing paralytic shellfish poison under different environmental conditions is extracted, a characteristic spectrum of the density of the algae producing the poison is extracted through a Db7 wavelet function, and the quantitative model of the algae producing the paralytic shellfish poison is established by adopting a stepwise regression analysis method based on the characteristic spectrum of the density of the algae producing the paralytic shellfish poison. The fluorescence characteristic standard spectrum library can be used for quantitatively analyzing the cell density of the microalgae producing paralytic saxitoxin. According to the invention, a paralytic shellfish producing quantitative model is established by a stepwise regression analysis method according to component fluorescence characteristic spectrums of a second scale and a third scale of a Db7 wavelet function, when toxic dinoflagellate is mixed with non-toxic dinoflagellate as a dominant species, the accuracy rates of density testing of the toxic dinoflagellate at high, medium and low concentrations are 98%, 67% and 90% respectively, and the aim of quantitatively analyzing the cell density of the toxin-producing algae is fulfilled.

Description

technical field [0001] The invention belongs to the field of identification and prevention of toxic microalgae, and particularly relates to a model for detecting the cell density of paralytic shellfish toxin-producing microalgae, a method for establishing the same, and applications. Background technique [0002] Red tide refers to the phenomenon that the microorganisms in the water multiply and cause discoloration due to the water environment and climate. In recent years, the occurrence frequency and area of ​​red tides in coastal areas of my country have been increasing continuously, resulting in serious economic losses. [0003] According to the toxicity characteristics of red tide, red tide is usually divided into three categories, namely non-toxic red tide, fish poisonous red tide and poisonous red tide. Among the three types of red tides, non-toxic red tides have the highest proportion. These types of red tides do not produce toxins. The toxins produced by fish-toxic r...

AI Technical Summary

Problems solved by technology

[0006] At present, there are some methods for detecting PSP, such as biological detection method, immunological method, and chemical analysis method, but these methods either have a single detection object, or are subject to the defects of poor accuracy, sensitivity, and repeatability, and cannot be widely used.

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