11 results about "Living sample" patented technology

The invention provides a nitric oxide fluorescent probe with aggregation-induced emission characteristics and a preparation method and application thereof. The structural formula of the nitric oxide fluorescent probe is shown in the specification. The nitric oxide fluorescent probe provided by the invention is high in detection sensitivity, high in response speed, excellent in NO selectivity and AIE characteristic, wide in application range, not only suitable for liquid-phase and gas-phase NO detection, but also capable of realizing high-efficiency real-time detection of NO in body fluid, cells and living body samples, and wide in application prospect. The method can be used for diagnosis of diseases such as inflammation (rheumatoid arthritis), tumor microenvironment and the like, high-throughput drug screening and environment monitoring.

The invention discloses a microneedle patch for in-vivo space-time omics analysis and a preparation method and application thereof, and belongs to the technical field of gene detection. Comprising the following steps: performing high-density modification on the surface of the microneedle for molecular binding; preparing a micro-needle base grinding tool for constructing a micro-needle patch; connecting the universal primer probe and the capture primer probe to the bar code probe; the microneedle patch captures to-be-detected molecules in a living body in situ; a probe complementary with the strand displacement region is added, polymeric strand displacement is carried out, and the obtained product can be used for constructing a sequencing library to realize spatial omics analysis of a living sample; the microneedle after strand displacement can be coupled with the bar code probe again to realize reuse of the microneedle for a new round of reaction. According to the method, the analysis of the spatial group in the living body sample is realized at extremely low cost, the operation is simple, the application field is wide, and the method can be used for detecting genomes, transcriptomes, proteomes, epigenomes, metabolomes and the like.

Systems and methods are provided for automated incubation of large numbers of tumor organoids or other volumetric biological samples cultured from cells or other tissues. Label-free, non-destructive imaging of the samples is performed quickly and at high temporal resolution (e.g., once per day or more), allowing interventions (like the addition or subtraction of substances like water, glucose, buffers, or pharmaceuticals) to be applied to each sample based on the images, facilitating closed-loop per-sample control of sample growth. This can lead to improved incubation outcomes, like increased rates of generation of live samples and control of sample size or other sample properties. Image and other incubation data obtained via such automated incubation processes can be used to train machine learning models to plan interventions for the samples, further increasing incubation success rates and allowing for the incubation of rare and / or novel samples, for which reliable incubation protocols do not yet exist.

A method for multi-parametric X-ray fluorescence imaging of a biological / living sample (10) containing a first marker substance with maximum detection sensitivity and minimum radiation dose, comprising the steps of: irradiating the sample (10) with X-ray radiation (1), wherein X-ray fluorescence (2) of the first marker substance is excited; spatially resolved detection of the X-ray fluorescence (2) of the first marker substance; and determining the distribution of the first marker substance in the sample (10) from the X-ray fluorescence (2) of the first marker substance, wherein the sample (10) contains at least one further marker substance which is excited by the X-ray radiation (1) into X-ray fluorescence (2), wherein the fluorescence lines (3) of the first marker substance and of the at least one further marker substance are different, at least one of the first marker substance and of the at least one further marker substance is coupled to active ingredient molecules and / or ligand molecules which are provided for a specific interaction with the sample (10) or are contained in cells, in order to be able to track these molecules, the detection comprises a spectrally resolved detection of the X-ray fluorescence (2) of the first marker substance and of the at least one further marker substance, and additionally at least one distribution of the at least one further marker substance in the sample (10) is determined from the detected X-ray fluorescence (2) of the first marker substance and of the at least one further marker substance. An imaging device (100) for multi-parametric X-ray fluorescence imaging and an optimized selection method for a marker substance kit for introducing marker substances into a sample (10) are also described.

The invention relates to a running water type live insect detection device and a detection method, which can realize automatic running water type live insect detection on the premise of not damaging insects as far as possible, provide a living sample basis for researches on behavior habits, growth and development and the like of the insects, help to deeply understand insect ecology and provide a reliable basis for accurate pest control. The running water type live insect detection system which is independently designed has good functionality and practicability. All important parts of the device are designed to be movable and detachable, the coupling of part connection is greatly improved through the structural design, the overall structure is more reasonable, and the overall operation efficiency of the system is effectively improved. The insect pest monitoring device has high realizability in the aspects of overall manufacturing and assembling, large-scale production, popularization and application are facilitated, the insect pest monitoring device can be rapidly put into actual agricultural production and insect pest monitoring work, and powerful support is provided for agricultural intelligent development.

This invention discloses a four-dimensional Raman monitoring system and dynamic quantitative method for environmental microplastics in vivo, belonging to the field of environmental ecological monitoring and analysis technology. The system includes a confocal spectroscopy acquisition module, a controller, and a data processing module. It acquires the three-dimensional coordinates of live samples through coordinated spatial scanning and simultaneously acquires Raman signals to construct four-dimensional spectral data. A multidimensional convolutional instance segmentation network is used to process the four-dimensional Raman spectral data, identifying and classifying endogenous biological matrices and exogenous multi-mixed microplastics in vivo, outputting voxel-level semantic classification results, and then quantitatively calculating the absolute retention volume and abundance of microplastics in the organism. This invention overcomes the shortcomings of existing technologies in label-free quantification under complex biological backgrounds, achieving in-situ accurate identification of mixed microplastics in vivo, and providing a standardized quantitative monitoring method for assessing the bioaccumulation, metabolic patterns, and ecotoxicological effects of environmental microplastics.

A method for statistically analyzing key factors affecting the spatiotemporal distribution of spawning of domesticated fish downstream of a reservoir mainly includes early fish resource monitoring, hydrological element monitoring, numerical simulation of river hydraulic conditions, and result analysis. Early fish resource monitoring mainly includes surface and mid-bottom layer sampling, on-site sample sorting and counting, culture and observation of live samples, long-term storage of dead samples after identification, cross-sectional coefficient verification, and sample accuracy statistics. Hydrological element monitoring mainly involves water level monitoring, water temperature measurement, flow velocity distribution measurement, flow measurement, and large-section measurement. The numerical simulation of river hydraulic conditions uses a two-dimensional hydrodynamic mathematical model to calculate the hydrodynamic conditions of the spawning ground river section. The result analysis correlates the hydrodynamic conditions within the calculation domain, the measured hydrological elements, and the spawning amount of domesticated fish, thereby clarifying the key factors affecting the spatiotemporal distribution of spawning of domesticated fish downstream of the reservoir. The advantage of the present invention is that, by statistically analyzing multi-source data, the key factors affecting the spatiotemporal distribution of spawning of domesticated fish downstream of the reservoir are identified, which is an effective way to accurately analyze the key factors affecting the spatiotemporal distribution of spawning of domesticated fish downstream of the reservoir.

The invention provides a quality detection method and system for sinonovacula constricta samples in different producing areas under control variables, and relates to the technical field of data processing.The method comprises the steps that on the basis of traceability information of different producing areas, representative sinonovacula constricta living samples are collected respectively, fishing time window synchronization control is carried out on the samples in all the producing areas, and the samples in all the producing areas are obtained; carrying out purification treatment in an environment with standardized temperature, salinity and temporary rearing duration, and executing unified cleaning, shelling and tissue separation pretreatment operation to obtain a variable-controlled standardized sample; and performing morphological and textural analysis on the standardized sample to obtain physical index data including individual specifications, shell surface color characteristics, shell meat mass ratio and muscle elasticity modulus. Accurate quality detection and production area traceability of sinonovacula constricta in different production areas under control variables can be realized.

The invention discloses a flow ploidy analysis kit, and relates to the technical field of biological detection. According to the flow ploidy analysis kit provided by the invention, the DAPI staining solution is specifically combined with the DNA double-helix minor groove, so that accurate marking of the genetic material of the cell nucleus is realized, and the flow ploidy analysis kit can be directly used for flow ploidy analysis and detection of animal cells. By optimizing the working concentration of the DAPI staining solution, the toxic effect of the dye on cells is reduced to the maximum extent while the signal intensity is guaranteed. The kit is suitable for trace living samples, ploidy analysis can be completed without killing animals, and lossless monitoring support is provided for polyploidy breeding. The kit provided by the invention adopts DAPI dyeing, does not need an illumination activation step, can complete the dyeing process within 5 minutes of incubation at room temperature, has the advantage that a fluorescence signal is slightly influenced by pH fluctuation, has the advantage of'detection while dyeing ', and is especially suitable for rapid screening in a field or resource limited scene.

The utility model relates to the technical field of zooplankton sorting, and discloses a zooplankton living body sample automatic sorting device which comprises a box body, partition plates are fixedly connected to the two sides of the inner wall of the box body, a sorting cavity is formed in an inner cavity, located between the partition plates, of the box body, and collecting cavities are formed in the box body and located on the two sides of the sorting cavity. A filtering assembly is mounted in an inner cavity of the sorting cavity and is used for filtering a living body sample; according to the automatic sorting device for zooplankter living body samples, a first filter plate is used for filtering, zooplankter living bodies stay in an inner cavity of a sorting cavity, along with filtering, the concentration of the living bodies in the inner cavity of the sorting cavity is increased, at the moment, the first filter plate moves upwards, so that the living bodies and water are driven to move upwards, and in the process, a pressing plate slides towards an inner cavity of a sleeve plate; the collecting cavity is opened, then high-concentration living bodies above the first filter plate can enter the inner cavity of the collecting cavity along with water, separation is easy and convenient, extra force is not applied to the living bodies, and the survival rate of the living bodies is increased.

The invention provides a double-light-trap method for multi-dimensional observation of biological particles and application of the double-light-trap method, and belongs to the technical field of microbiomechanics research. According to the invention, the biological particles are taken as a symmetry point, two symmetrical light traps are arranged on two sides of the biological particles by using the optical tweezers system, the edge of the biological particles is located at a 1 / 4 position of a light spot, and real-time dynamic multi-dimensional observation is carried out on the biological particles by adjusting the scanning frequency of the optical tweezers system and the laser power of the light traps. Three-dimensional control can be achieved through the synergistic effect of the double optical traps, millisecond-level controllable overturning is achieved, the rapid observation requirement of a living body sample is met, the capturing efficiency can reach 100%, the activity of the biological sample is not damaged, and a brand new research normal form is provided for microbiomechanics.