Field environment test system and method for small aquatic animals

A technology for aquatic animals and environmental testing, applied in biological testing, material inspection products, climate change adaptation and other directions, can solve the problems that cannot play a role in combating immobilization, cannot achieve long-term exposure to aquatic animals, and biological feeding, etc., to prevent watershed pollution. The effect of accidents, ensuring the health of the watershed ecosystem, and preventing the loss of bait

Active Publication Date: 2016-03-30
TONGJI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the organisms were not fed during the experiment, and long-term exposure studies of aquatic animals could not be realized
Moreover, the depth adjustment component is installed on the top of the in-situ test device, and the test cult

Method used

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  • Field environment test system and method for small aquatic animals

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Effect test

Embodiment 1

[0037] A kind of small-scale aquatic animal field environment test system, comprises support 8 and the breeding box 4 that is fixed on the support 8, and breeding box 4 adopts long 50cm * wide 50cm * high 30cm plexiglass to make, as figure 1As shown, according to the needs of the test, the system is set up at different depths at the same point in the water body to set up three experimental groups, the first experimental group 1, the second experimental group 2 and the third experimental group 3, and each experimental group includes three Breeding box 4, three breeding boxes 4 of an experimental group are arranged on the same level of the same point of the water body, and each experimental group is set on different levels of the same point of the water body, wherein the first experimental group 1 is set on the surface water body layer (water body surface within the range of 5 below 10), the third experimental group 3 is set in the sediment layer at the bottom of the water body (...

Embodiment 2

[0043] The small aquatic animal field environment online observation system in this embodiment is basically the same as that in Embodiment 1. In this embodiment, the system is used to face aquatic animals at two different water body levels near the sewage outlet and two different water body locations away from the sewage outlet water area. Toxicity studies are achieved through the following steps:

[0044] (1) a set of this system is set respectively near the sewage outlet and far away from the water area of ​​the sewage outlet, and the model organism zebrafish cultivated in the laboratory is cultivated in each breeding box 4 of the system, ensuring that the side hole 5 of the breeding box is smaller than that cultivated in the laboratory The individual size of the model organism zebrafish;

[0045] (2) Feed the model organism zebrafish cultivated in the laboratory through a feeding catheter, record the behavioral characteristics of aquatic animals through a photographic devic...

Embodiment 3

[0048] The small aquatic animal field environment online observation system of the present embodiment is basically the same as that in Embodiment 1, and the system is used for the toxicity research of aquatic animals at different water body levels at the same site in different seasons: through the following steps:

[0049] (1) The system is set at the site of the water body to be studied in each season, and the model organism zebrafish cultivated in the laboratory is cultivated in each culture box 4 of the system, ensuring that the side hole 5 of the culture box is smaller than the model cultivated in the laboratory The individual size of biological zebrafish;

[0050] (2) Feed the model organism zebrafish cultivated in the laboratory through a feeding catheter, record the behavioral characteristics of aquatic animals through a photographic device, take eight weeks as a test period, and continue for one test period;

[0051] (3) After a test period, the number of living aquati...

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Abstract

The invention relates to a field environment test system for small aquatic animals. The system comprises a support and culture boxes fixed on the support; the side faces of the culture boxes are made of a material with multiple holes, the tops and the bottoms of the culture boxes are made of a continuous material, and feeding guide pipes are arranged in the culture boxes. A method adopting the system for a test comprises the steps that the system is placed in a testing water area according to the test requirements, the height direction of the support is consistent with the water depth direction, and the small aquatic animals are cultured in the culture boxes; the small aquatic animals are fed through the feeding guide pipes, the behavior characteristics of the aquatic animals are recorded through photography devices, and the step is continuously performed for one test cycle; the survival number of the small aquatic animals is counted, living samples of the small aquatic animals are collected, quickly frozen by adopting liquid nitrogen and taken back, and the influence of a water body and sediment in the testing water area on the toxic effect of the small aquatic animals is detected. Compared with the prior art, the influence of the in-situ water body on the behavioral and physiological effects of the tested animals from the different time and space levels can be researched.

Description

technical field [0001] The invention belongs to the field of environmental monitoring, and relates to a small aquatic animal field environment test system and method, in particular to a small aquatic animal field environment long-term exposure test and behavior change online observation system and method. Background technique [0002] At present, all over the world, pollutants such as domestic sewage and industrial wastewater discharge, atmospheric deposition, and non-point source pollution of pesticides cause water pollution to varying degrees. Water pollutants include heavy metals such as lead, mercury, and cadmium, and persistent organic pollutants such as organochlorine pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers. Most of these pollutants have lethal, teratogenic and Carcinogenic "three-cause effect" and environmental estrogen effects. Therefore, the toxicological research of water pollutants has received widespread attention all over the w...

Claims

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Application Information

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IPC IPC(8): A01K61/00G01N33/48
CPCY02A40/81A01K61/00G01N33/48
Inventor 吴玲玲黄清辉陈玲郜洪文李倩
Owner TONGJI UNIV
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