Miniaturized self-adaptive optical two-photon fluorescent imaging system and method

A technology of two-photon fluorescence and adaptive optics, which is applied in the fields of application, medical science, and diagnosis by using light. It can solve the problems of large size and inability to apply miniaturized two-photon microscopes, and achieve high spatial and temporal resolution.

Active Publication Date: 2017-08-11
PEKING UNIV
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Problems solved by technology

However, since conventional adaptive optics systems require complex wavefront detection devices and wavefron

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  • Miniaturized self-adaptive optical two-photon fluorescent imaging system and method
  • Miniaturized self-adaptive optical two-photon fluorescent imaging system and method
  • Miniaturized self-adaptive optical two-photon fluorescent imaging system and method

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Embodiment Construction

[0022] In the drawings, the same or similar reference numerals are used to denote the same or similar elements or elements having the same or similar functions. Embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] In the description of the present invention, the terms "central", "longitudinal", "transverse", "front", "rear", "left", "right", "vertical", "horizontal", "top", " The orientation or positional relationship indicated by "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying the referred device or element Must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be construed as limiting the scope of the invention.

[0024] The living sample of the pre...

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Abstract

The invention discloses a miniaturized self-adaptive optical two-photon fluorescent imaging system and a miniaturized self-adaptive optical two-photon fluorescent imaging method. The imaging system comprises a laser light source device, a miniature probe device, a wavefront detecting device, a de-scanning component and a fluorescent imaging device, wherein the laser light source device is used for outputting exciting light; the miniature probe device is used for receiving the exciting light output by the laser light source device, generating a fluorescent signal, and carrying out wavefront correction on various isoplanatic regions according to the average wavefront distortion distribution of various isoplanatic regions of a tissue plane imaging view field inside a living sample; the wavefront detecting device is connected with the miniature probe device for detecting the average wavefront distortion distribution; the de-scanning component is used for compensating the isoplanatic region off-axis effect generated by the miniature probe device in real time before the wavefront detecting device detects the average wavefront distortion distribution; the fluorescent imaging device is used for collecting the fluorescent signal output by the miniature probe device, and thus the imaging of the tissue plane inside the living sample is completed. With the system and the method, the large-visual-field, high-temporal-spatial-resolution and deep biological tissue imaging can be realized on an animal moving freely.

Description

technical field [0001] The invention relates to the technical field of two-photon fluorescence imaging, in particular to a miniaturized adaptive optical two-photon fluorescence imaging system and method. Background technique [0002] Direct recording of neuronal activity in freely moving animals is one of the most direct and effective methods to study the relationship between animal behavior and neural function. For a long time, this task was performed by an electrophysiological approach. In recent years, optical imaging and especially fluorescence microscopy have played an increasingly important role in this task. Compared with electrophysiological methods, the biggest advantage of optical imaging is its non-invasiveness, larger imaging field of view and more observable targets. Compared with ordinary single-photon fluorescence imaging technology, two-photon microscopy has better optical sectioning ability and deeper penetration depth. This makes two-photon microscopy th...

Claims

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Application Information

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IPC IPC(8): A61B5/00
CPCA61B5/0059Y02A40/70
Inventor 宗伟健程和平陈良怡张云峰王爱民
Owner PEKING UNIV
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